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New medicinal application of andrographolide

A technology of andrographolide and drugs, applied in the field of medicine, can solve the problems of andrographolide in the treatment of malignant glioma and its mechanism of action, etc.

Inactive Publication Date: 2012-04-04
SHENYANG PHARMA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no reports on the application and mechanism of andrographolide in the treatment of malignant glioma.

Method used

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  • New medicinal application of andrographolide
  • New medicinal application of andrographolide
  • New medicinal application of andrographolide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1. The inhibitory effect of andrographolide on the growth of malignant glioma U251 and U87 cells in vitro

[0017] 1 Experimental method

[0018] U251, U87 cells adherent growth in the medium containing 10% fetal bovine serum, 100U / ml penicillin, 100μg / ml streptomycin, 0.2% NaHCO 3 RPMI-1640 culture medium, placed in 5% CO 2 , saturated humidity, and 37°C incubator, and the culture medium was changed every other day for subculture.

[0019] Take the adherent tumor cells in the logarithmic growth phase and make 6×10 4 cells / mL concentration, 100 μl per well was inoculated in a 96-well plate and cultured overnight. After the cells were fully adhered to the wall, the original culture medium was removed, and different concentrations of andrographolide (provided by the Natural Medicine Research Laboratory of Shenyang Pharmaceutical University), PI3K / Akt signal-specific inhibitor LY294002 (5 μM, 10 μM, 20 μM, positive control) or DMSO (final concentratio...

Embodiment 2

[0023] Example 2. Inhibitory effect of andrographolide on tumor growth in animal model bearing glioma in vivo

[0024] 1 Experimental method

[0025] Fifteen six-week-old female nude mice were purchased from the Chinese Academy of Military Medical Sciences and raised in a sterile system with constant temperature and humidity. Divided into three groups, blank control group, DMSO group and andrographolide (Andro) administration group, 8 rats in each group. Take U251 cells and adjust to a cell concentration of 3×10 7 / ml, the cell viability was detected by trypan blue, and the cell survival rate was >90%. The above suspension was drawn up with a syringe, and 100 μl of the single cell suspension was subcutaneously injected on the lateral side of the right hind limb. Five days after inoculation, the nude mice in the three groups were measured with a vernier caliper to measure the long and short diameters of the tumors, and then the corresponding doses of drugs were given ...

Embodiment 3

[0028] Example 3, andrographolide can induce malignant glioma U251, U87 cell cycle arrest in G 2 / M phase to inhibit cell growth;

[0029] 1 Experimental method

[0030] In order to confirm the mechanism of andrographolide inhibiting the proliferation of human malignant glioma U251 and U87 cells, the changes of cell cycle distribution after different concentrations of andrographolide were investigated by flow cytometry. U251, U87 cells at 1×10 6 Cells / ml were planted in a culture flask, and the cells were collected 72 hours after adding different concentrations of andrographolide, centrifuged at 1000 rpm / centrifuge for 5 minutes, carefully discarded the supernatant, added 500 μl PBS to suspend the cells, and added 10 ml of 70% ice-cold ethanol and fixed overnight at -20°C. Centrifuge the sample before detection at 1000 rpm / centrifuge for 5 minutes, carefully discard the supernatant, add 10 μl of RNAase (200 μg / ml) to each sample, mix well, and act for 30 minutes at ...

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Abstract

The invention belongs to the technical field of medicines, and relates to new medicinal application of andrographolide, in particular to new application of the andrographolide in preparation of medicines for treating spongioblastoma. The andrographolide can restrain the growth of human spongioblastoma cells in vivo and in vitro, the mechanism of the andrographolide relates to down regulation of activation of a PI3K / Akt signal, and then human andrographolide cell cycles are induced to be retarded in a G2 / M period. The andrographolide can be used for preparing intestinal canal and parenteral administration dosage forms and is used for treating human spongioblastoma.

Description

technical field [0001] The invention belongs to the technical field of medicine, relates to a new medical application of andrographolide, in particular to its new application in the preparation of drugs for treating glioma. Background technique [0002] Malignant glioma is the most common intracranial malignant tumor, and the effect of surgical treatment and radiotherapy is not satisfactory. Chemotherapy is currently the main means of clinical treatment of malignant glioma. The pathogenesis of malignant glioma involves abnormalities in the structure and function of a variety of proto-oncogenes and tumor suppressor genes, and is essentially a polygenic abnormality disease. It is characterized by abnormal regulation of normal growth, unlimited proliferation and inhibition of apoptosis, resulting in abnormal accumulation of cells and even tumor formation. Literature has shown that the PI3K / Akt signaling pathway is overactivated in malignant glioma cells. [0003] Th...

Claims

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Application Information

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IPC IPC(8): A61K31/365A61P35/00
Inventor 马恩龙肖伟李艳春邱峰张鹏飞杨星刚
Owner SHENYANG PHARMA UNIVERSITY
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