Preparation method for measles attenuated live vaccine

A technology of live attenuated vaccine and measles virus, applied in the field of vaccines, can solve the problems of easy inactivation of measles virus, poor uniformity of vaccine quality, and ineffectiveness of vaccines, and achieve easy large-scale production, increase single batch yield, and uniform quality and stable effect
CN102406933AInactive Publication Date: 2012-04-11CHENGDU KANGHUA BIOLOGICAL PROD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
CHENGDU KANGHUA BIOLOGICAL PROD
Publication Date
2012-04-11
Estimated Expiration
Not applicable · inactive patent
Patent Text Reader

Abstract

The present invention relates to the field of vaccine, especially to a preparation method for a measles attenuated live vaccine. The method comprises the following steps: culturing in a microcarrier bioreactor; culturing by a maintenance media; harvesting and purifying; preparing the vaccine and freeze drying. With adopting the high density culture method of the bioreactor, it is ensured that the resulting virus titer is higher than the virus titer from the conventional process; on the premise of the high virus titer by the method of the present invention, a molecular sieve chromatography method or a hollow fiber filtering method is further adopted to carry out purification to remove most of impurity components, such that the generation of the side reactions of the vaccination is effectively avoided. In addition, with adopting the bioreactor to culture the virus, the culture conditions are stable, such that the large-scale production is easy to realize, and the single batch production of the vaccine is increased so as to ensure the quality uniformity and the quality stability of the product.
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Description

technical field

[0001] The invention relates to the field of vaccines, in particular to a preparation method of live attenuated measles vaccine. Background technique

[0002] Measles is an ancient infectious disease caused by infection with the measles virus. At present, vaccination with live attenuated measles vaccine is an effective means to prevent and control the occurrence of the disease. The effect of live attenuated measles vaccine is mainly based on the survival and reproduction of live virus in the body. Because measles virus is very sensitive to light and heat, it is easy to be inactivated, and its stability is related to the temperature of the virus environment and the protein content in the virus fluid. It is not easy to be inactivated during the process and is prepared into a freeze-dried dosage form.

[0003] However, although the current measles vaccine has some control over the bovine serum albumin content of the virus harvest in terms of production, it do...

Claims

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