Bifidobacterium bifidum anti-freeze culture medium and application method thereof

A Bifidobacterium bifidum and culture medium technology is applied in the field of Bifidobacterium bifidum antifreeze medium, which can solve the problems of low number of viable bacteria, inconvenience, and easy water absorption of freeze-dried bacterial powder, etc. Trendy, easy-to-use effect

Inactive Publication Date: 2012-04-11
SHAANXI RES INST OF AGRI PRODS PROCESSING TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a kind of bifidobacterium bifidum antifreeze medium and application method thereof, this antifreeze medium solves the problem that the freeze-drying survival rate of bifidobacterium bifidum exists in the freeze-drying process is not high, live bacteria The number is low, the freeze-dried bacteria powder is easy to absorb water and moisture, and it is inconvenient to use in non-dairy products such as preparations, capsules, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Accurately weighed 18g lactose, 4g yeast extract, 7g sodium chloride, 10g soybean peptone, 1.5g diammonium hydrogen citrate, 2.0g dipotassium hydrogen phosphate, 0.15g MgSO 4 Dissolve 1.0ml Tween 80 in 950mL distilled water, heat to dissolve and mix evenly, adjust the pH to 6.2 with 1mol / L NaOH, and then put it in an anaerobic bottle, the filling volume is 90%, and put it on the rubber stopper of the anaerobic bottle cap Insert a 1ml syringe needle, then sterilize at 121°C for 15 minutes, pull out the syringe needle after sterilization, and cool to room temperature to obtain medium A; (2) Accurately weighed 0.5g sodium ascorbate, 0.6g sorbitol, 0.3 G glutamic acid, 4.0g cysteine ​​hydrochloride and 0.3g betaine are dissolved in 50ml distilled water, then filter and sterilize with a 0.22μm filter membrane to obtain the filtrate; , mix the filtrate evenly with culture medium A with a sterile syringe to obtain the antifreeze culture medium for Bifidobacterium bifidum. ...

Embodiment 2

[0020] (1) Accurately weighed 20g lactose, 5g yeast extract, 6g sodium chloride, 8g soybean peptone, 2.0g diammonium hydrogen citrate, 1.0g dipotassium hydrogen phosphate, 0.2g MgSO 4 Dissolve 1.0ml Tween 80 in 950mL distilled water, heat to dissolve and mix evenly, adjust the pH to 6.8 with 1mol / L NaOH, and then put it in an anaerobic bottle, the filling volume is 90%, and put it on the rubber stopper of the anaerobic bottle cap Insert a 1ml syringe needle, then sterilize at 118°C for 20 minutes, pull out the syringe needle after sterilization, and cool to room temperature to obtain medium A; (2) Accurately weighed 0.8g sodium ascorbate, 0.8g sorbitol, 0.4 1g of glutamic acid, 3.5g of cysteine ​​hydrochloride and 0.5g of betaine were dissolved in 50ml of distilled water, and then sterilized with a 0.22 μm filter membrane to obtain the filtrate; , mix the filtrate evenly with culture medium A with a sterile syringe to obtain the antifreeze culture medium for Bifidobacterium bi...

Embodiment 3

[0023] (1) Accurately weighed 17g lactose, 3g yeast extract, 9g sodium chloride, 8g soybean peptone, 1.0g diammonium hydrogen citrate, 2.0g dipotassium hydrogen phosphate, 0.2g MgSO 4 Dissolve 0.5ml Tween 80 in 950mL distilled water, heat to dissolve and mix evenly, adjust the pH to 6.5 with 1mol / L NaOH, and then put it in an anaerobic bottle, the filling volume is 90%, and put it on the rubber stopper of the anaerobic bottle cap Insert a 1ml syringe needle, then sterilize at 119°C for 18 minutes, pull out the syringe needle after sterilization, and cool to room temperature to obtain medium A; (2) Accurately weighed 0.4g sodium ascorbate, 1.0g sorbitol, 0.2 G glutamic acid, 5.0g cysteine ​​hydrochloride and 0.4g betaine are dissolved in 50ml distilled water, then filter and sterilize with a 0.22 μm filter membrane to obtain the filtrate; , mix the filtrate evenly with culture medium A with a sterile syringe to obtain the antifreeze culture medium for Bifidobacterium bifidum. ...

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PUM

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Abstract

The invention provides a bifidobacterium bifidum anti-freeze culture medium, which includes the following components by weight: 16-20 g of lactose, 3-5 g of yeast extract, 6-9 g of sodium chloride, 0.6-1.0 g of sorbitol, 0.2-0.5 g of glutamic acid, 8-12 g of soybean peptone, 0.5-2.0 g of diammonium hydrogen citrate, 0.2-0.5 g of betaine, 1.0-2.5 g of dipotassium phosphate, 0.4-0.8 g of sodium ascorbate, 0.1-0.2 g of MgSO4, 0.5-1.0 ml of Tween-80, 3-5 g of cysteine hydrochloride and 1,000 ml of distilled water. In the bifidobacterium bifidum anti-freeze fungal powder is prepared by using the anti-freeze culture medium, the freeze-dry survival rate is over 58 percent, and viable count of fungal powder is over 2.0*10<11> cfu / g; the freeze-dry survival rate of the fungal powder prepared by MRS (de Man, Rogosa and Sharp) broth is 8.74 percent at most; and the bifidobacterium bifidum anti-freeze culture medium does not contain dried skim milk or trehalose, so that the problem of high probability of water absorption and moistening in storage of the fungal powder is solved.

Description

technical field [0001] The invention belongs to the technical field of production of bifidobacterium products, and relates to a bifidobacterium bifidum antifreeze culture medium for improving freeze-drying survival rate and an application method thereof. Background technique [0002] Bifidobacteria (Bifidobacteria) was first isolated in 1899 by the French scholar Tissier from the feces of breast-fed infants. Bifidobacteria. There are 36 species of bifidobacteria that have been discovered so far, of which 12 species are isolated from the human body, and the Ministry of Health stipulates that 5 species can be used in health food, namely Bifidobacterium bifidum (B.bifidum), Bifidobacterium breve (B.breve), Bifidobacterium longum (B.longum), Bifidobacterium adolescentis (B.adolescentis), Bifidobacterium infantis (B.infantis). Bifidobacteria accompany the whole life of the human body, and only decrease or even disappear under illness, aging or other unfavorable conditions. As ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/04C12R1/01
Inventor 舒国伟马振兴陈合马齐秦涛张华雷小兰
Owner SHAANXI RES INST OF AGRI PRODS PROCESSING TECH
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