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Rhodococcus sp. for removing nitrite nitrogen from sewage at low temperature condition, and separation and culture method thereof

A technology of nitrite nitrogen and low temperature conditions, applied in the direction of microorganism-based methods, separation of microorganisms, chemical instruments and methods, etc., can solve the problem of loss of activity, decline in water quality of sewage treatment plant effluent, and the number cannot reach the status of dominant bacteria, etc. question

Active Publication Date: 2012-04-25
GUOZHONG AIHUA TIANJIN MUNICIPAL ENCIRONMENT ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the process of temperature drop, the metabolic activity of mesophilic bacteria gradually decreases, and they lose their activity when they reach a certain temperature. However, the number of low-temperature microorganisms suitable for low-temperature environments cannot be reduced in number due to their own physiological characteristics and the inhibition of various ecological factors. Reaching the status of the dominant flora, resulting in a decline in the quality of effluent from sewage treatment plants in winter

Method used

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  • Rhodococcus sp. for removing nitrite nitrogen from sewage at low temperature condition, and separation and culture method thereof
  • Rhodococcus sp. for removing nitrite nitrogen from sewage at low temperature condition, and separation and culture method thereof

Examples

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Effect test

Embodiment 1

[0022] A method for isolating and cultivating Rhodococcus for removing nitrite nitrogen in sewage under low temperature conditions, comprising the following steps:

[0023] (1) Inoculate the sludge of 15mL Tianjin Jizhuangzi Sewage Treatment Plant aeration tank into the Erlenmeyer flask that 250mL nitrobacteria nutrient solution is housed, and the nitrogen concentration in the sodium nitrite in the described nitrobacterial nutrient solution is 50mg / L , at 10°C, the shaking table speed is 130rpm, and when the degradation rate of nitrite nitrogen reaches 90%, let it stand still, remove the supernatant, and inoculate the sludge at the bottom of the Erlenmeyer flask to a 250mL second In the first kind of nitrobacteria culture solution, the nitrite nitrogen concentration in the second kind of nitrite bacteria culture solution is 150mg / L, at 10 ℃, in the shaking table that rotating speed is 130rpm, acclimation, until the degradation rate of nitrite nitrogen reaches When 90%, promptl...

Embodiment 2

[0041] Degradation Effect of Nitrite-1 on Nitrite Nitrogen

[0042] In a 1L beaker, add 500mL of nitrate bacteria culture solution, adjust the initial nitrite nitrogen concentration to 50mg / L, inoculate Nitrogen-1 of the present invention, connect three rings of Nitrobacter-1 of the present invention from the Nitrobacter solid plate 1 into the above-mentioned nitric acid bacteria culture solution, keep the pH as 7.5, measure the degradation situation of nitrite nitrogen by Nitrate-1 of the present invention in 72 hours, the results are shown in figure 2 .

[0043] Depend on figure 2 It can be seen that after 72 hours of degradation, the concentration of nitrite nitrogen in the solution is 6.2 mg / L, and the nitrite nitrogen is basically removed, with a removal rate of 87.6%, which proves that Nitrite-1 in the present invention has the effect on nitrite nitrogen. Has good removal ability.

Embodiment 3

[0045] A method for isolating and cultivating Rhodococcus for removing nitrite nitrogen in sewage under low temperature conditions, comprising the following steps:

[0046] (1) Inoculate the sludge of 10mL Tianjin Jizhuangzi sewage treatment plant aeration tank into the conical flask that 250mL nitrobacteria nutrient solution is housed, the nitrogen concentration in the sodium nitrite in the described nitrobacterial nutrient solution is 50mg / L , at 8°C, the shaking table speed is 140rpm, and when the degradation rate of nitrite nitrogen reaches 80%, let it stand still, remove the supernatant, and inoculate the sludge at the bottom of the Erlenmeyer flask to a 250mL second In a kind of nitrate bacteria culture solution, the nitrite nitrogen concentration in the second kind of nitrite bacteria culture solution is 150mg / L, at 8 ℃, in the shaking table that rotating speed is 140rpm, acclimation, treat that nitrite nitrogen degradation rate reaches 80%, promptly obtain the nitrate ...

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Abstract

The present invention discloses Rhodococcus sp. for removing nitrite nitrogen from sewage at a low temperature condition, and a separation and culture method thereof. The Rhodococcus sp. xiaoshen-1 CGMCC No.5277 for removing the nitrite nitrogen from the sewage at the low temperature condition has the function of removal of the nitrite nitrogen from the sewage at the low temperature and aerobic condition. The experimental results show that: the significant reinforcement effect of removal of the nitrite nitrogen in the sewage at the low temperature and especially at the temperature of 8-12 DEGC is provided despite the significant inhibition of the low temperature environment after the Rhodococcus sp. xiaoshen-1 CGMCC No.5277 of the present invention is added to the sewage, wherein the nitrite nitrogen removal rate in the sewage inoculated with the Rhodococcus sp. of the present invention is increased by about 10% compared to the nitrite nitrogen removal rate in the sewage without inoculation with the Rhodococcus sp. of the present invention.

Description

technical field [0001] The invention relates to a rhodococcus strain, its use and its isolation and cultivation method. Background technique [0002] The reason why low temperature has a significant impact on biological treatment capacity is that the decrease in temperature affects the growth and metabolism of microorganisms and changes the microbial flora in sewage treatment systems. In the process of temperature drop, the metabolic activity of mesophilic bacteria gradually decreases, and they lose their activity when they reach a certain temperature. However, the number of low-temperature microorganisms suitable for low-temperature environments cannot be reduced in number due to their own physiological characteristics and the inhibition of various ecological factors. Reaching the status of the dominant flora, which leads to a decline in the quality of the effluent of the sewage treatment plant in winter. Therefore, there is an urgent need for a bacterial strain that can r...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02C02F3/34C02F3/02C12R1/01C02F101/16
CPCY02W10/10
Inventor 田青姜秀光郝赟陈爱因
Owner GUOZHONG AIHUA TIANJIN MUNICIPAL ENCIRONMENT ENG
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