Pectin preparation method
A technology of pectin and acid hydrolysis, which is applied in the direction of digestive system, metabolic diseases, antineoplastic drugs, etc., can solve the problems of discarding and waste of resources, and achieve the effects of simple operation, environmental pollution and high extraction rate
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[0015] The preparation method of the present invention is:
[0016] 1. Raw material pretreatment
[0017] Take fresh citrus peels, chop them to a particle size of 2-3 mm, heat-treat in boiling water for 5 minutes to inactivate pectinase, soak in 70% ethanol for 30 minutes, the ratio of solid to liquid is 1:15-1:20, and the pH is 0.1-2.0, 70 Maintain at ~90°C for 5 minutes, press filter, remove pigments and other alcohol-soluble impurities, wash the residue with 95% ethanol, and then wash with ether to avoid the loss of water-soluble pectin, air-dry ether, and dry at 45°C to constant weight;
[0018] 2. Acid hydrolysis extraction
[0019] Add hydrochloric acid solution to the pretreated raw materials, heat in a constant temperature water bath, shake well, and centrifuge until the supernatant is not viscous. The extraction temperature is 70-90°C, and the extraction time is 60-120 minutes. The supernatant is Pectin extract, dilute the supernatant to 100mL.
Embodiment 1
[0020] Example 1: The ratio of solid to liquid is 1:20, the pH is 0.1, the extraction temperature is 70° C., the extraction time is 120 min, and the extraction rate is 20.45%.
Embodiment 2
[0021] Example 2: The solid-liquid ratio is 1:15, the pH is 2.0, the extraction temperature is 80° C., the extraction time is 60 min, and the extraction rate is 21.68%.
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