Molecular identification method of trichothecene type-A toxins of fusarium

A fusarium and toxin technology, which is applied in the field of molecular identification of fusarium trichothecenes A toxins, can solve the problems that the toxin-producing species have not been verified by chemical analysis, the results cannot be consistent with each other, and the reliability is not high.
CN102443590BInactive Publication Date: 2014-06-18HUAZHONG AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
HUAZHONG AGRI UNIV
Publication Date
2014-06-18
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention belongs to the technical field of plant inspection and quarantine, and specifically relates to a molecular identification method of trichothecene type-A toxins of fusarium. The molecular identification method of the invention is characterized by comprising the steps of: designing a pair of specific primers through Tril13 gene information aiming at the trichothecene type-A toxins; separating the fusarium which generates type-A toxins of the fusarium to obtain specific segments (the length is 470 bp) of T-2 toxins, wherein the specific segments cannot be amplified in the fusarium which does not generate the type-A toxins of the fusarium; and adopting PCR (Polymerase Chain Reaction) reaction and utilizing a product from agarose gel electrophoresis to determine whether one fusarium can generate T-2 toxins directly according to existence and size of a product segment. The molecular identification method disclosed by the invention can be used for detecting trichothecene type-A toxins of fusarium in grains, feed and food safety.
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Description

Technical field

[0001] The invention belongs to the technical field of plant inspection and quarantine, and specifically relates to a molecular identification method of Fusarium trichothecenes A toxin. Background technique

[0002] Fusarium head blight or scab (Fusarium head blight or scab) is an important disease that harms wheat, barley, corn and other crops worldwide. It not only seriously affects crop yields, but also Fusarium can produce a variety of toxic effects to humans and animals. The role of trichothecenes toxins reduces the quality of food or feed. Fusarium toxin has high stability and is not easily degraded. It can enter the food chain during food and feed processing, thereby threatening human and animal health. These toxins can inhibit the protein synthesis of eukaryotic cells and destroy the immune system of humans and animals. The poisoning is often accompanied by symptoms such as vomiting, diarrhea, and dizziness. Some toxins may even cause cancer. Trichothece...

Claims

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