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Production technology of D-aspartic acid and L-alanine

A technology of aspartic acid and alanine, which is applied in the field of bioengineering, can solve the problems of large consumption of auxiliary raw materials and low substrate concentration, and achieve the effects of improving production efficiency, concentration and yield

Inactive Publication Date: 2012-05-09
ANHUI HUAHENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method focuses on the cultivation of strains, the substrate concentration is low, the consumption of auxiliary raw materials is large, and the conversion reaction and extraction process need to be further optimized

Method used

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  • Production technology of D-aspartic acid and L-alanine

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Take the enzyme activity as 2240μmol·ml -1 h -1 Add 1692kg of ammonium DL-aspartate to 2000L of fermentation broth, add water to make a 5000L reaction solution, in which the content of DL-aspartic acid is 30%, react at 45°C for 48h, control pH5.5 and continuously add DL-aspartate Acid 1525kg. The concentration of D-aspartic acid in the reaction solution was about 30%, and a small amount of L-alanine crystals were precipitated.

[0021] The reaction solution was centrifuged with a 1000-rpm three-legged centrifuge, washed with a small amount of water, and the crystals were collected and dried to obtain 324 Kg of L-alanine. The filtrate was cooled to 30°C and passed through a tubular ultrafiltration membrane. The filtrate was treated with 0.5% activated carbon decolorized and filtered, and the filtrate was washed with H 2 SO 4 Adjust the pH to 2.8, cool to 25°C, centrifuge, wash and dry to obtain 1388kg of D-aspartic acid. Add 350kg of calcium oxide (CaO) to the remai...

Embodiment 2

[0024] Take the enzyme activity as 2490μmol·ml -1 h -1 Add 2256kg of DL-aspartic acid ammonium to 2000L of fermentation liquid, add water to make 5000L of reaction liquid, in which DL-aspartic acid content is about 40%, react at 48°C for 65h, control pH6.0 and continuously add DL-aspartic acid Acid 1987kg. The concentration of D-aspartic acid in the reaction solution was about 40%, and a small amount of L-alanine crystals were precipitated.

[0025] The reaction solution was centrifuged with a 1000-rpm three-legged centrifuge, washed with a small amount of water, and the crystals were collected and dried to obtain 682 Kg of L-alanine. The filtrate was cooled to 30°C and passed through a tubular ultrafiltration membrane. The filtrate was treated with 0.5% activated carbon decolorized and filtered, and the filtrate was washed with H 2 SO 4 Adjust the pH to 2.9, cool to 25°C, centrifuge, wash and dry to obtain 1734kg of D-aspartic acid. Add 450kg of calcium oxide (CaO) to th...

Embodiment 3

[0028] Take the enzyme activity as 2160μmol·ml -1 h -1 Add 2538Kg of ammonium DL-aspartate to 2000L of fermentation broth, add water to make 5000L of reaction liquid, in which DL-aspartic acid content is about 45%, react at 50°C for 72h, control pH6.2 and continuously add DL-aspartic acid Acid 2300kg. The concentration of D-aspartic acid in the reaction solution was about 45%, and a small amount of L-alanine crystals were precipitated.

[0029] The reaction solution was centrifuged with a 1000-rpm three-legged centrifuge, washed with a small amount of water, and the crystals were collected and dried to obtain 826 kg of L-alanine. The filtrate was cooled to 30°C and passed through a tubular ultrafiltration membrane. The filtrate was treated with 0.5% activated carbon decolorized and filtered, and the filtrate was washed with H 2 SO 4 Adjust the pH to 2.8, cool to 25°C, centrifuge, wash and dry to obtain 1956kg of D-aspartic acid. Add 660kg calcium hydroxide (Ca(OH) 2 ), p...

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Abstract

The invention relates to a production technology of D-aspartic acid and L-alanine. With DL-aspartic acid ammonium as a substrate, the technology utilizes a fermentation liquid rich in L-aspartic acid-beta-decarboxylase to conduct a conversion reaction so as to produce D-aspartic acid and L-alanine. During the reaction process, fed-batch of DL-aspartic acid is performed continuously to control the pH value of the reaction system, and the substrate concentration can also be doubled, so that finally the concentration of D-aspartic acid in the system can reach 50%. For the generated mother liquid, the technology makes the following treatments: first recovering the L-alanine precipitated from crystallization through centrifugation, then using H2SO4 to adjust isoelectric points so as to separate out D-aspartic acid crystals, then utilizing calcium salt precipitation method to extract L-alanine dissolved in the mother liquid, collecting the mother liquid for circulation of isoelectric points and calcium salt precipitation combined extraction. By means of fed-batch of the substrate, the technology of the invention can produce D-aspartic acid and L-alanine of high concentration, has acid and base consumption reduced by half during production, and has the advantages of simple extraction process, high yield, as well as substantially reduced cost.

Description

technical field [0001] The invention relates to a production process of D-aspartic acid and L-alanine, belonging to the field of bioengineering. Background technique [0002] D-aspartic acid preparation technology mainly includes chemical method and enzymatic method: [0003] The chemical asymmetric resolution method (Special Publication No. 52-54190) has a complicated process, and the product D-aspartic acid has low optical purity and has no industrial value. [0004] In the enzymatic production of D-aspartic acid technology, there is an asymmetric degradation method (Zhao 56-20753), which uses Candida to degrade L-aspartic acid in DL-aspartic acid to obtain D-aspartic acid Aspartic acid; L-aspartic acid-α-decarboxylase method (CN1242054A), using genetic engineering technology to construct L-aspartic acid-α-decarboxylase-producing bacteria, simultaneously producing D from DL-aspartic acid -aspartic acid and β-alanine; and the simultaneous preparation of D-aspartic acid a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/20C12P13/06C07C229/08C07C229/24C07C227/42
Inventor 不公告发明人
Owner ANHUI HUAHENG BIOTECH
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