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Bioreactor and sophorolipid continuous production method by using the same

A technology of bioreactor and microbial fermentation biosurfactant, which is applied in the method of biological material sampling, bioreactor/fermenter combination, bioreactor/fermenter for specific purposes, etc., can solve the problem that there is no public sale of pure microbial fermentation biosurfactant. , high production costs, restrictions on sophorolipids, etc., to achieve the effects of easy equipment control, reduced separation costs, and increased output

Inactive Publication Date: 2012-06-13
中海石油环保服务(天津)有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The application range of sophorolipids is very wide, but its production cost remains high, which limits the large-scale commercial production process of sophorolipids. At present, there is no large-scale sophorolipid production enterprise in my country, and there is no international scope. Public sale of pure sophorolipids
Factors restricting the industrial production of sophorolipids mainly include the production capacity of strains, culture conditions, production methods, and separation and extraction methods. Among them, research on strain mutagenesis and optimization of culture conditions has been very common, and relatively good strains have also been found. , high and stable yields can be achieved under suitable culture conditions, but in general, there is still a big gap between the existing technology and the low cost required for industrialized production

Method used

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  • Bioreactor and sophorolipid continuous production method by using the same
  • Bioreactor and sophorolipid continuous production method by using the same
  • Bioreactor and sophorolipid continuous production method by using the same

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Taking the strain 1 whose sophorolipid yield was 90 g / L during batch fermentation as the production strain as an example.

[0025] Add 6L of sterilized fermentation medium into the device, and the components of the medium are glucose 130g / L, yeast powder 10g / L, and urea 1g / L. Add the cultured seed liquid through the inoculation port, the seed liquid is the bacterial cell enrichment liquid obtained by culturing the thalline through the seed medium at 30°C for 48 hours, the culture condition: the pH value is 6; the composition of the seed medium is : Glucose 100g / L, yeast powder 10g / L and urea 1g / L; then add rapeseed oil in batches at a rate of 50mL / d. Add rapeseed oil in batches at a high speed. 96 hours before the fermentation, the upper and lower tanks were ventilated with oxygen at the same time, and the rotating speed was controlled to keep the dissolved oxygen above 50%. Ferment until the aeration at the lower end stops for 96 hours, open the valve after 5 hours, ...

Embodiment 2

[0028] Take the strain 2 whose sophorolipid yield is 120g / L during batch fermentation as the production strain as an example.

[0029] Add 6L sterilized fermentation medium into the device, the medium components are glucose 150g / L, yeast powder 3g / L, peptone 0.7g / L, sodium citrate 5g / L, MgSO 7H O 4g / L, ( NH4)2SO42g / L, KH2PO41g / L, NaCl 0.1g / L, CaCl2·2H2O 0.1g / L. Add the cultured seed liquid through the inoculation port, the seed liquid is the bacterial cell enrichment liquid obtained by culturing the thalline through the seed medium at 30°C for 48 hours, the culture condition: the pH value is 6; the composition of the seed medium is : Glucose 100g / L, yeast powder 10g / L and urea 1g / L; then add rapeseed oil in batches at a rate of 80mL / d. 55 hours before the fermentation, the upper and lower tanks were ventilated with oxygen at the same time, and the rotating speed was controlled to keep the dissolved oxygen at a relatively high level. Ferment until the aeration at the lower en...

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Abstract

According to a bioreactor and a sophorolipid continuous production method by using the same provided by the invention, as fed-batch of a fermentation broth, a product is recovered at a liquid outlet through intravenous drip. A technology mainly comprises the following steps: centrifuging a fermentation waste liquid by a centrifuge and recovering precipitated yeast byproduct at a lower layer; mixing a centrifuged supernatant and a crude product from intravenous drip and recovering a lower layer product to a secondary purified product gathering tank 2-5 h after settlement layering; disinfecting the secondary purified product by an autoclave and storing directly, or drying at 55-75 DEG C in a drying tower to gain a constant weight and storing.

Description

technical field [0001] The invention relates to a bioreactor and a method for continuously producing sophorolipids. Background technique [0002] When a large-scale oil spill occurs, especially when the weather conditions at sea are bad, it is very difficult to recover the spilled oil using physical methods such as oil booms and oil skimmers. In this case, the oil spill dispersant can accelerate the natural dispersal process of the oil, and it is easier to break the oil into small oil droplets under the action of sea waves, accelerating the natural biodegradation of the oil spill. The use of oil spill dispersants has gradually become widely accepted and most coastline countries rely on dispersants when responding to oil spills. [0003] Dispersants can be divided into chemical degreasers and biological degreasers. Due to the high production cost of biosurfactant, the main component of biological dispersants, the large-scale application of biological dispersants is hindered...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12M1/04C12M1/02C12P19/12
CPCC12M33/22C12M47/12C12M23/34C12M27/02C12M47/14C12M45/05
Inventor 李广茹曲良陈宇卢文玉杨雪朱零青
Owner 中海石油环保服务(天津)有限公司
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