Application of enhancer Hr3 for promoting Hycu-EP32 protein increment expression

Abstract

The invention relates to the transgenic technology of bombyx mori, in particular to the application of enhancer Hr3 for promoting Hycu-EP32 protein increment expression. The increment expression vector takes a transposition vector pBac [3 * P3 - EGFPafm] as the base vector; and the transposition vector is sequentially connected with enhancer Hr3, a 39kP promotor, a Hycu-EP32 gene and a termination signal sequence. In the invention, transgenic increment expression exogenous antiviral protein is used for preparing anti-BmNPV bombyx mori, which is the first method of improving the resistance of bombyx mori by adopting transgenic increment expression exogenous resistant protein in diapause bombyx mori; the Hycu-EP32 protein can be expressed in each growth period, so as to overcome the defect that the normal bombyx mori cannot express Hycu-EP32, and provide convenience for inhibiting viral breeding by utilizing Hycu-EP32 protein in each period; and the expressed increment of protein increases with viral increment, so as to reduce impact of expression exogenous protein on normal physiological activities and economic characters of bombyx mori, and obviously improve the resistance of bombyx mori.

Description

technical field [0001] The invention relates to the field of genes, in particular to the transgenic technology of silkworm. Background technique [0002] Transgenic technology is the introduction of artificially isolated and modified genes into the genome of organisms, resulting in the heritable modification of the traits of organisms due to the expression of the introduced genes. Transgenic technology is an important technology in modern biological research, and plays an important role in functional gene research, biological material innovation, modern genetic breeding, etc. [0003] The silkworm is a Lepidoptera insect with important economic value. The silk industry generates more than 10 billion yuan in income for silkworm farmers every year. The silk industry has made important contributions to the world's economic, cultural and social development. However, when the silkworm encounters a virus, it usually causes huge losses to the sericulture industry. According to st...

AI Technical Summary

Problems solved by technology

However, the anti-virus strains prepared by using polymorphic silkworm varieties through transgenic interference have obvious defects in the following two aspects: ①The species used for production are generally diphelic diapause species, and the anti-virus strains prepared with polymorphic silkworm varieties It is not conducive to production promotion, and compared with diapause silkworm varieties, polymorphism silkworm varieties do not have a diapause period, and the maintenance of their subcultures can only rely on continuous breeding and breeding, which requires a lot of manpower and material resources to obtain. The maintenance and subculture of transgenic silkworm strains also greatly increases the risk of resource loss of obtained transgenic silkworm varieties

②Transgenic interference strains can only function at the mRNA level, but cannot function at the level of viral DNA replication and protein synthesis

Up to now, the method of using diapause silkworm varieties to increase the expression of exogenous antiviral proteins and transgenic lines has not been reported at home and abroad

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