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Cultivation and quality control method for ixeris sonchifolia hance for Kudiezi injection

A quality control method, the technology of Kudou stalks, which is applied in the field of cultivation and quality control of Kudishi injections, can solve the problems of low natural reproduction rate, difficulty in recovering drug sources, etc. Low seed germination rate and other problems, achieve the effect of shortening field management time and growth cycle, significant social and ecological benefits, and shortening the growth cycle

Active Publication Date: 2012-07-04
沈阳双鼎制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Due to the diameter of the seeds of P. phallis about 0.1 mm and the low germination rate of the seeds, the natural reproduction rate of P. chinensis in wild conditions is very low, the growth is slow, and it is difficult to restore the source of medicine.
But still do not have a kind of ideal technology of artificially cultivating Kudiezi Injection at present to use Kudie

Method used

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  • Cultivation and quality control method for ixeris sonchifolia hance for Kudiezi injection
  • Cultivation and quality control method for ixeris sonchifolia hance for Kudiezi injection
  • Cultivation and quality control method for ixeris sonchifolia hance for Kudiezi injection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] A method for artificially cultivating Kudiezi injection, a Chinese herbal medicine, Kusheria spp., comprising the following process steps:

[0059] 1. Seedling cultivation in greenhouse

[0060] 1), seed treatment

[0061] ① Seed harvesting: choose strong plants, when most of the achenes turn black, harvest the seeds when the seeds are dark brown or brown; pull out the plants, put them in a cool and ventilated place, ripen them for 3 to 5 days, thresh them, and dry them in the sun. cleaning, packaging;

[0062] ②Seed storage: After harvesting the seeds of A. chinensis, put them in cloth bags when the moisture content of the seeds is 10-13%, and put 20g / kg / bag of asarum inside. The storage room requires a cool and dry place; The storage period of seeds generally does not exceed 3 years;

[0063] ③ Seed treatment: variable temperature treatment during storage;

[0064] a. Temperature change treatment of seeds: In the second month before sowing, the storage temperature...

experiment example 1

[0091] Determination method of total flavonoids in medicinal materials of A.

[0092] 1. Instruments and reagents used

[0093] Instrument: SHIMADZU UV-1800 UV-visible spectrophotometer and workstation.

[0094] Reagent: The reagent is of analytical grade, and the water is water for injection. Luteolin-7-O-β-D-glucuronide, self-made, the content detected by HPLC normalization method is greater than 98%.

[0095] 2. Establishment of measurement wavelength

[0096]After drawing the ultraviolet absorption spectrum of the reference substance solution prepared according to the method, the reference substance solution has the maximum absorption at 506nm, so 506nm is the wavelength for the determination of the total flavonoid content in the sample.

[0097] 3. Investigation on the linear range of luteolin-7-O-β-D-glucuronide reference substance

[0098] Precisely weigh 10.24mg of luteolin-7-O-β-D-glucuronide reference substance, put it in a 25ml measuring bottle, add 60% ethanol ...

experiment example 2

[0128] Determination method of total phenolic acid content in the medicinal material of A.

[0129] 1. Instruments and reagents used

[0130] Instrument: SHIMADZU UV-1800 UV-visible spectrophotometer and workstation.

[0131] Reagent: The reagent is of analytical grade, and the water is water for injection. Chichoric acid, China Institute for the Control of Biological Products, the content detected by HPLC normalization method is greater than 98%.

[0132] 2. Establishment of measurement wavelength

[0133] After drawing the ultraviolet absorption spectrum of the reference substance solution prepared according to the method, the reference substance solution has the maximum absorption at 746nm, so 746nm is the wavelength for the determination of the total flavonoid content in the sample.

[0134] 3. Investigation on standard curve of cichoric acid reference substance

[0135] Accurately weigh 6.08mg of cichoric acid reference substance, put it in a 10ml brown measuring bott...

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Abstract

The invention discloses a cultivation and quality control method for ixeris sonchifolia hance for Kudiezi injection, which is characterized by including seedling raising, transplanting, field management and harvesting. A method of quality control for ixeris sonchifolia hance is also studied and includes a method of colorimetric determination for large-class active ingredients such as flavone and total polyphenol in ixeris sonchifolia hance, and a method of multi-component quantitation by one marker for liquid gradient elution of cichoric acid, luteoline-7-O-beta-D-glucuronide and caftaric acid. By the technique of cultivation of ixeris sonchifolia hance, planting and harvesting of two crops a year are achieved, land resources are saved, yield per unit area is increased to 100%-150%, and quality of the ixeris sonchifolia hance meets the requirement of medicinal materials for traditional Chinese medicine injection.

Description

technical field [0001] The invention relates to the technical field of planting Chinese herbal medicines, in particular to a cultivation and quality control method of Kudiezi Kudiezi injection. Background technique [0002] Ixeris sonchifolia (Bge.) Hance] is a plant of the genus Ixeris sonchifolia in the family Asteraceae, and is mainly produced in Northeast and North my country. It has been recorded that the bitter root of the plant has the effects of clearing heat, detoxifying, detoxifying, and relieving pain. Folks are mainly used to treat appendicitis, tonsillitis, and unknown swelling and pain. In recent years, with the rapid development of modern extraction, separation and spectroscopy technology and modern pharmacology, the research on its chemical composition and biological activity has become more and more in-depth. [0003] The chemical composition in A. cumulus is relatively complex, mainly containing flavonoids, organic acids, phenolic acids, sesquiterpene lac...

Claims

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Application Information

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IPC IPC(8): A01G1/00G01N21/31G01N30/02
Inventor 马占芝王学东王松林生可心于玲玲
Owner 沈阳双鼎制药有限公司
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