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Azotobacter-containing microbial fertilizer

A microbial fertilizer and microbial technology, applied in the direction of organic fertilizers, can solve problems such as environmental pollution, achieve the effects of preventing pests and diseases, improving the survival rate of seedlings, and improving disease resistance

Active Publication Date: 2012-07-04
THE IT ELECTRONICS ELEVENTH DESIGN & RES INST SCI & TECHNOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This shows that it is limited to increase crop yields only by increasing the application of chemical fertilizers in large quantities, not to mention a series of problems such as environmental pollution.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Brown spherical nitrogen-fixing bacteria culture medium:

[0069] First level training

[0070] Sucrose 1.5%, potassium dihydrogen phosphate 0.05%, calcium carbonate 0.05%, magnesium sulfate 0.05%, sodium chloride 0.02%, agar 3%. PH 7-7.2, culture at 25-27°C for 2-3 days.

[0071] secondary medium

[0072] Remove the agar at the first level, and cultivate at 25-27°C for 3-5 days.

[0073] Tertiary medium

[0074] The composition of the medium is calculated by mass percentage: 0.5% sodium acetate, 0.5% peptone, 0.05% sodium chloride, 0.05% dipotassium hydrogen phosphate, 0.05% magnesium sulfate, 0.05% sodium carbonate, and the balance is water. Culture at 25-27°C for 3-5 days.

Embodiment 2

[0076] Bacillus jelly-like

[0077] First level training

[0078] Sucrose 1.5%, potassium dihydrogen phosphate 0.05%, calcium carbonate 0.05%, magnesium sulfate 0.05%, sodium chloride 0.02%, yeast extract 0.05%, agar 3%, and the balance is water. Incubate at 25-27°C for 2-3 days.

[0079] Secondary training:

[0080] Remove the agar from the primary medium, and culture at 25-27°C for 3-5 days.

[0081] Tertiary medium

[0082] The medium components are calculated by mass percentage: 0.5% sodium acetate, 0.5% peptone, 0.05% sodium chloride, 0.05% dipotassium hydrogen phosphate, 0.05% magnesium sulfate, and 0.05% sodium carbonate. Adjust the pH value, inoculate the secondary culture strains in the seed tank, and cultivate at 25-27°C for 5-7 days.

Embodiment 3

[0084] Photosynthetic bacteria

[0085] First level training

[0086] The medium components are calculated by mass percentage: 0.5% yeast extract, 0.5% peptone, 0.05% sodium chloride, 0.05% dipotassium hydrogen phosphate, 0.05% magnesium sulfate, 0.05% sodium carbonate, and the balance is water;

[0087] PH6~10, temperature 28-35℃, light intensity 3000-4000 lux, time 6-8 days.

[0088] Secondary training

[0089] Add 0.1% ferric ammonium citrate to the primary medium, the fermentation temperature is 28°C~35°C, and the culture time is 5-7 days;

[0090] Tertiary training

[0091] The medium components are calculated by mass percentage: 0.5% sodium acetate, 0.5% peptone, 0.05% sodium chloride, 0.05% dipotassium hydrogen phosphate, 0.05% magnesium sulfate, 0.05% sodium carbonate, and the balance is water;

[0092] Inoculate the secondary cultured bacteria into the seed tank, pH 7~8, temperature 26-35°C, light intensity 3000-4000 lux, for 6-8 days; then inoculate in the fermen...

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Abstract

The invention discloses an azotobacter-containing microbial fertilizer. The azotobacter-containing microbial fertilizer consists of microbial fermentation broths in percentage by volume; microbes are photosynthetic bacteria, azotobacter chroococcum, phosphate-solubilizing bacillus megaterium, jelly-like bacilli, actinomycetes and lactic acid bacteria; and the volume ratio of the microbial fermentation broths is 1 to 15:2 to 10:5 to 9:1 to 8:2 to 10:3 to 8. The microbial fertilizer can fix nitrogen, solubilize phosphate, increase the survival rate of seedlings and the germination rate of seeds, effectively prevent diseases and pests and enhance the disease resistance of plants.

Description

technical field [0001] The invention relates to a microbial fertilizer and belongs to the field of planting. Background technique [0002] Microbial fertilizer is a product in which the life activities of microorganisms cause crops to obtain specific fertilizer effects, and it is a type of fertilizer used in agricultural production. It has a history of nearly 50 years in China. Microbial fertilizer is a living fertilizer, and its function is mainly completed by the life activities of a large number of beneficial microorganisms it contains. Only when the life activities of these beneficial microorganisms are completed. Only when these beneficial microorganisms are vigorously multiplying and metabolizing, can material transformation and beneficial metabolites be continuously formed. Therefore, the types of beneficial microorganisms in microbial fertilizers and whether their life activities are vigorous are the basis of their effectiveness, unlike other fertilizers that are ...

Claims

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Application Information

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IPC IPC(8): C05F11/08
Inventor 赵振元罗明树王明云
Owner THE IT ELECTRONICS ELEVENTH DESIGN & RES INST SCI & TECHNOLOGICAL ENG
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