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Method of culturing coriolus versicolor-induced oxalate decarboxylase through jerusalem artichoke carbon source

A technology of Coriolus versicolor and oxalate decarboxylase, which is applied in the field of preparation of oxalate decarboxylase, can solve the problems of inability to cultivate and low economic value, and achieve the effects of easy operation, stable enzyme production and simple method

Inactive Publication Date: 2012-07-04
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main use of Jerusalem artichoke in our country is to pickle and eat pickles, which leads to low economic value and cannot be widely cultivated

Method used

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  • Method of culturing coriolus versicolor-induced oxalate decarboxylase through jerusalem artichoke carbon source
  • Method of culturing coriolus versicolor-induced oxalate decarboxylase through jerusalem artichoke carbon source
  • Method of culturing coriolus versicolor-induced oxalate decarboxylase through jerusalem artichoke carbon source

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Production of Coriolus versicolor from Jerusalem artichoke juice (the initial pH value of the medium is 5.0)

[0032] Fresh Jerusalem artichoke was steamed at 105°C for 15 minutes, then water was added at a mass ratio of 1:1, and homogenized to obtain Jerusalem artichoke pulp. The Jerusalem artichoke pulp was leached at 80°C for 1 hour, filtered through two layers of gauze, and centrifuged at 8000r / min for 10 minutes to obtain Jerusalem artichoke juice. The total sugar content of the Jerusalem artichoke juice was determined by the phenol-sulfuric acid method (using glucose as the standard sugar) at 4°C. Save it for later use. Add Jerusalem artichoke juice containing 1g total sugar (calculated as glucose) to every 50mL fermentation medium, then add 0.15g peptone, 0.05g potassium dihydrogen phosphate, 0.025g MgSO 4 ·7H 2 O, 0.01g Na 2 HPO 4 12H 2 O, and 50 μL of trace elements (the formula of trace elements is: FeSO 4 ·7H 2 O 10g / L, MnSO 4 ·H 2 O 1.0g / L, ZnSO 4 ...

Embodiment 2

[0035] Production of Coriolus versicolor from Jerusalem artichoke juice (the initial pH value of the medium is 5.0)

[0036] Fresh Jerusalem artichoke was steamed at 105°C for 20 minutes, then water was added at a mass ratio of 1:5, and homogenized to obtain Jerusalem artichoke pulp. The Jerusalem artichoke pulp was leached at 90°C for 1.5h, filtered through two layers of gauze, centrifuged at 8000r / min for 10min to obtain Jerusalem artichoke juice, and the total sugar content of the Jerusalem artichoke juice was determined by the phenol sulfuric acid method (using glucose as the standard sugar), at 4°C Store under conditions. Add Jerusalem artichoke juice containing 0.35g total sugar (calculated as glucose) to every 50mL fermentation medium, then add 0.15g peptone, 0.05g potassium dihydrogen phosphate, 0.025g MgSO 4 ·7H 2 O, 0.01g Na 2 HPO 4 12H 2 O, and 50 μL of trace elements (the formula of trace elements is: FeSO 4 ·7H 2 O 10g / L, MnSO 4 ·H 2 O 1.0g / L, ZnSO 4 ·7H...

Embodiment 3

[0039] Production of Coriolus versicolor from Jerusalem artichoke juice (the initial pH value of the medium is 5.0)

[0040] Fresh Jerusalem artichoke was steamed at 105°C for 30 minutes, then water was added at a mass ratio of 1:10, and homogenized to obtain Jerusalem artichoke pulp. The Jerusalem artichoke pulp was extracted for 2 hours at 110°C, filtered through two layers of gauze, and centrifuged at 8000r / min for 10 minutes to obtain Jerusalem artichoke juice. The total sugar content of the Jerusalem artichoke juice was determined by the phenol sulfuric acid method (using glucose as the standard sugar), and the condition was 4°C. Save it for later use. Add Jerusalem artichoke juice containing 1-4g total sugar (calculated as glucose) to every 50mL fermentation medium, then add 0.15g peptone, 0.05g potassium dihydrogen phosphate, 0.025g MgSO 4 ·7H 2 O, 0.01g Na 2 HPO 4 12H 2 O, and 50 μL of trace elements (the formula of trace elements is: FeSO 4 ·7H 2 O 10g / L, MnSO ...

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Abstract

The invention relates to a method of culturing coriolus versicolor-induced oxalate decarboxylase through jerusalem artichoke carbon source, which comprises: (1) lixiviating jerusalem artichoke juice. (2) diluting the jerusalem artichoke juice to the total sugar content thereof is 7-100g / L, adding nitrogen source and inorganic salt to obtain fermentation medium, adjusting the pH value of fermentation medium to 4.0-5.0, sterilizating for preparation; (3) bringing the mycelium seed liquid of coriolus versicolor into fermentation medium for culturing two to six days; (4) when mycelium pellets are formed, adding oxalic acid with final concentration 1-100mM to induce oxalate decarboxylase, and obtaining mycelium after culturing 0.5-6 days; (5) breaking up the frozen mycelium, extracting mycelium fragments with acetate buffer, removing the residue, and obtaining oxalate decarboxylase crude enzyme. The invention has simple process, low cost, high efficiency, non-inhibition of substrate induced oxalate decarboxylase, and stable enzyme production.

Description

technical field [0001] The invention belongs to the field of preparation of oxalate decarboxylase, in particular to a method for inducing oxalate decarboxylase by culturing Coriolus versicolor as a carbon source from Jerusalem artichoke. Background technique [0002] Oxalate decarboxylase can catalyze the decarboxylation of oxalate to form formic acid and carbon dioxide, so it has broad application prospects in the enzymatic detection of oxalate content, the degradation of oxalate in industrial wastewater, the preparation of low-oxalate-content foods, and the reduction of oxalate concentration in the human body. Coriolus versicolor is an important strain for producing oxalate decarboxylase, how to reduce its culture cost, produce mycelium in large quantities, and induce mycelium to synthesize oxalate decarboxylase is an important issue. Since carbon is the most abundant element in organisms, the carbon source is the most used in the medium and accounts for a relatively high ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/88C12R1/645
Inventor 洪枫宫搏阳杨雪霞
Owner DONGHUA UNIV
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