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Preparation method for magnetic nano biological microspheres for remedying soil polluted by organic chloride

A magnetic nanometer technology for polluted soil, applied in the recovery of polluted soil, fixed on/in organic carrier, fixed on or in inorganic carrier, etc., to achieve small diffusion limit, simple production, high activity. Effect

Inactive Publication Date: 2012-07-04
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There have been some studies on immobilizing various biological enzymes on carriers for biomedicine, food brewing or sewage treatment by immobilization technology, but there is no report on immobilizing degrading bacteria with magnetic nanospheres for bioremediation of polluted soil

Method used

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  • Preparation method for magnetic nano biological microspheres for remedying soil polluted by organic chloride
  • Preparation method for magnetic nano biological microspheres for remedying soil polluted by organic chloride

Examples

Experimental program
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Effect test

Embodiment 1

[0037] (1) Preparation of nano-Fe by co-precipitation method 3 O 4 , the co-precipitation method uses ammonia water as a precipitant, and adds FeCl under nitrogen protection. 3 with FeCl 2 , where Fe 3+ : Fe 2+ : OH - The molar ratio is 2:1:8, and the chemical reaction equation is: 2Fe 3+ +Fe 2+ +8OH - =Fe 3 O 4 +4H 2 O; after the reaction, it was freeze-dried to obtain the nano-Fe adsorbed on the surface of the amino group. 3 O 4 particles;

[0038] (2) Dissolve 1 g of chitosan in 100 mL of acetic acid solution with a mass fraction of 15% to form a uniform and transparent colloidal solution, and then dissolve 5 g of the prepared nano Fe 3 O 4 The particles were added and stirred for 20 min to obtain magnetic nanoparticles, which were repeatedly washed with distilled water until neutral, and dried for later use;

[0039] (3) adding the prepared magnetic nanoparticles into a 0.1M citric acid-phosphoric acid buffer solution with pH=5.0, and ultrasonically dispersi...

Embodiment 2

[0045] The difference from Example 1 is that:

[0046] The magnetic nanoparticles are obtained by dissolving 2 g of chitosan in 100 mL of acetic acid solution with a mass fraction of 20% to form a uniform and transparent colloidal solution, and then dissolving 10 g of the prepared nano Fe 3 O 4 Granules were added and stirred for 30min.

[0047] The prepared magnetic nano-biological microspheres were added at a rate of 5%. When the initial concentration of the organochlorine herbicide atrazine in the soil was 30 mg / kg, the restoration was performed for 35 days, and the removal rate of atrazine was 65%.

Embodiment 3

[0049] The difference from Example 1 is that:

[0050] The mass concentration of the magnetic fluid is 0.4 g / mL, pH=6.0 citric acid-phosphate buffer solution.

[0051] The bacterial species is Bacillus sp. and the bacterial concentration is about 10 times the volume of the magnetic fluid. 6 bacteria / ml of bacterial solution.

[0052] The cross-linking agent is 2% sodium alginate with a mass concentration twice the volume of the magnetic fluid, the cross-linking time is 1 h, and the cross-linking temperature is 30°C.

[0053] The prepared magnetic nano-biological microspheres were added at a rate of 2%. When the initial concentration of the organochlorine herbicide 2,4-D in the soil was 30mg / kg, the removal rate of 35d, 2,4-D was 55%. .

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Abstract

The invention relates to a preparation method for magnetic nano biological microspheres for remedying soil polluted by organic chloride durably. The preparation method comprises the following steps of: (1) preparing nano Fe3O4 particles with the surfaces adsorbing amino by a coprecipitation method; (2) dissolving chitosan into an acetic acid solution to form a homogeneous transparent colloidal solution, and mixing and stirring the solution and the nano Fe3O4 particles to obtain the magnetic nano-particles; (3) adding the prepared magnetic nano-particles into a buffering solution of citric acid and performing ultrasonic dispersion to obtain magnetic fluid; and (4) adding the magnetic fluid into a bacterial solution, adding cross-linked fluid dropwise, performing adsorption-cross-linking, and performing solid liquid separation under the action of an external magnetic field to obtain the magnetic nano biological microspheres. The method is quick, simple, convenient and low in cost. The prepared magnetic nano biological microspheres have the characteristics of homogenous shape, large specific surface area, high microbial activity and the like and are applicable to degradation of organic chloride pollutant and in-situ remediation of polluted soil.

Description

technical field [0001] The invention relates to the in-situ restoration of persistent organochlorine-contaminated soil, in particular to a preparation method of magnetic nano-biological microspheres used for the restoration of organochlorine-contaminated soil. Background technique [0002] Organic chlorides are a class of highly toxic and refractory environmental pollutants. Traditional treatment technologies have shortcomings such as incomplete degradation and low efficiency. The development of nanotechnology has brought a new and promising method to the treatment of organochlorine pollutants, that is, the use of nano-reducing metal particles to dehalogenate the organochloride to reduce its toxicity. Currently, with Fe 2+ and Fe 3+ The technology of synthesizing nano-iron particles from common raw materials has been mature, and the nanoparticles also show efficient degradation performance of organochlorine pollutants. More importantly, it can be flexibly applied to in si...

Claims

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Application Information

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IPC IPC(8): C12N11/14C12N11/10B09C1/10
Inventor 司友斌张进霞司雄元刘小红
Owner ANHUI AGRICULTURAL UNIVERSITY
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