Kit for jointly detecting four deafness predisposing genes and application thereof

Abstract

The invention discloses a fluorescent detection kit for detecting four deafness predisposing genes simultaneously. The kit comprises reagents before amplification and reagents after amplification, wherein the reagents before amplification comprise a polymerase chain reaction (PCR) buffer solution, a reaction mixture of MgCl2 and deoxyribonucleoside triphosphates (DNTPs), Taq DNA polymerase, ultrapure water, and a primer mixture for amplifying loci of GJB2235delC, 12S rRNA 1555A>G mutation, 1494C>T mutation, IVS7-2A>G and Amelogenin at high specificity; and the reagents after amplification comprise a genotyping standard and an internal standard. Deafness gene loci of the GJB2235delC, 12S rRNA 1555A>G mutation, 1494C>T mutation, IVS7-2A>G and Amelogenin are simultaneously detected at high sensitivity and high specificity by combining a fluorescent labeling technology, a linolenic acid (LNA) nucleoside monomer doping-primer modification technology and a capillary electrophoresis technology for the first time, manpower and material resources and time are greatly saved, and pollution due to multi-step operation is prevented.

Description

technical field [0001] The invention relates to a fluorescent detection kit for simultaneously detecting four deaf susceptibility genes, belonging to the technical field of biological detection. Background technique [0002] Worldwide studies on the pathogenic factors of hearing and speech disabilities show that about 60-80% of the patients are caused by genetic factors, and clinical research data from developed countries show that hereditary deafness accounts for about 80% of deafness patients. Therefore, in the past ten years, the research on the pathogenesis and molecular epidemiology of hereditary deafness has become one of the most important contents of deaf research. With the completion of the Human Genome Project, great progress has been made in the positioning and cloning of deafness genes. The molecular genetics research and molecular epidemiological data of deafness have enabled researchers to gradually realize that mutations in susceptibility genes for deafness pl...

AI Technical Summary

Problems solved by technology

These methods have different defects, such as cumbersome operation, difficult interpretation of results, poor repeatability, many false negatives and false positives, etc.

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