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Bioenzyme-loaded calcium phosphate bone cement for preventing and treating bone infection and preparation method thereof

A technology of biological enzyme calcium phosphate and calcium phosphate bone cement, which is used in medical preparations containing active ingredients, pharmaceutical formulations, medical science and other directions to achieve the effect of preventing and treating bacterial infections

Active Publication Date: 2015-10-07
SHANGHAI HI TECH UNITED BIO TECHCAL RES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] One of the technical problems to be solved in the present invention is to provide a controlled-release enzyme-loaded calcium phosphate bone cement to overcome the defects of existing calcium phosphate bone cement preparations

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Preparation of Calcium Phosphate Bone Cement Loaded with Lysostaphin

[0027] 1) Preparation of sodium alginate-lysostaphin complex suspension

[0028] Take 0.1 g of lysostaphin and dissolve in 1 ml of water. Another 0.1 g of sodium alginate was dissolved in 10 ml of water. Mix and stir 1ml of lysostaphin solution and 1ml of sodium alginate solution to obtain sodium alginate-lysostaphin complex suspension.

[0029] 2) Preparation of calcium phosphate cement

[0030] Mix 57mg of tricalcium phosphate and 43mg of calcium hydrogen phosphate powder evenly, add 30μl of calcium chloride aqueous solution (10%), reconcile and stir evenly, put the resulting paste in a mold, and after demoulding, place it at 37°C, 100% Fully solidified for 24 hours under relative humidity to obtain calcium phosphate bone cement.

[0031] 3) Preparation of calcium phosphate bone cement loaded with lysostaphin

[0032] Soak the calcium phosphate bone cement obtained in step 2) into th...

Embodiment 2

[0033] Embodiment 2 Preparation of calcium phosphate bone cement loaded with lysozyme

[0034] 1) Preparation of sodium alginate-lysozyme complex suspension Take 0.1 g of lysozyme and add 1 ml of water to dissolve it. Another 0.1 g of sodium alginate was dissolved in 10 ml of water. Mix and stir 1ml of lysostaphin solution and 1ml of sodium alginate solution to obtain sodium alginate-lysozyme complex suspension.

[0035] 2) Preparation of porous calcium phosphate cement

[0036] Mix 57mg of tricalcium phosphate, 43mg of calcium hydrogen phosphate powder and 10mg of solid paraffin (passed through a 40-mesh sieve), and add 30 μl of calcium chloride aqueous solution (10%), mix well, place the resulting paste in a mold, and remove After molding, place it at 37°C and 100% relative humidity for full curing for 24 hours, put it in absolute ethanol for 12 hours at 70°C, change the absolute ethanol constantly, take it out and dry it in an oven to obtain porous calcium phosphate bone ...

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Abstract

The invention belongs to the technical field of medicaments and particularly relates to a biological enzyme-carrying calcium phosphate bone cement with a controlled release effect and a preparation method thereof. The preparation disclosed by the invention comprises the following components in percentage by weight: 25 to 90 percent of calcium phosphate bone cement substrate, 0.001 to 50 percent of biological enzyme composite and 5 to 50 percent of curing liquid. When the biological enzyme-carrying calcium phosphate bone cement preparation is implanted, the biological enzyme with an antibacterial effect is released suddenly and then is stably released, so the biological enzyme-carrying calcium phosphate bone cement has the effect of filling and repairing bone defect as well as the effects of preventing and treating bacterial infection, and can be used for treating chronic osteomyelitis, preventing implanted part infection and preventing relapse after a bone tumor is removed.

Description

technical field [0001] The invention belongs to the field of biological preparations, in particular to an enzyme-loaded calcium phosphate bone cement with controlled release and a preparation method thereof. Background technique [0002] Bone defect caused by trauma, tumor and other reasons is a difficult problem in clinical treatment. The aseptic state of the implant site is the premise and key to the success of bone grafting. Clinical studies have shown that if the site to be implanted is infected, the treatment effect will not be good, especially for large and medium-sized orthopedic operations. Once infection occurs, the patient's mortality rate will increase, the hospitalization time will be prolonged, and the hospitalization cost will increase significantly. Due to the irrational use of antibiotics, drug-resistant bacteria are gradually increasing in clinical practice. In recent years, the research focus of new antibacterial drugs at home and abroad has begun to turn...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/48A61L27/54A61L27/56A61L27/12
Inventor 黄青山崔福英蒋司嘉陆婉英
Owner SHANGHAI HI TECH UNITED BIO TECHCAL RES
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