Preparation method of metal nanoparticle dimer

A metal nanoparticle, dimer technology, applied in nanostructure manufacturing, biochemical equipment and methods, nanotechnology, etc., can solve the problems of low assembly yield, complicated purification steps, etc., and achieve easily controllable conditions and simple operation. , good repeatability

Active Publication Date: 2012-07-11
SUZHOU NAFANG TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to propose a method for preparing a high-purity metal nanoparticle di

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  • Preparation method of metal nanoparticle dimer
  • Preparation method of metal nanoparticle dimer
  • Preparation method of metal nanoparticle dimer

Examples

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Example Embodiment

[0031] Example 1: Add the active intermediate DHLA-NHS and the amino-modified single-stranded primers P30 and P30C into an aqueous solution containing 70% acetonitrile (P30 and P30C are oligonucleotides containing 30 bases and have complementary sequences ), and add a small amount of triethylamine as acid binding agent, react for 12 hours. The DHLA-modified primers P30 and P30C were concentrated using vacuum evaporation equipment, and the acetonitrile component was evaporated.

[0032] Prepare a dimer of 13nm symmetrical gold nanoparticles linked by 30bp dsDNA. Under equimolar conditions, DHLA-P30 and DHLA-P30C are slowly lowered to room temperature from 95℃ in 1×PBS buffer for paired hybridization into double strands dsP30, add equimolar dsP30 to a 13nm gold nanoparticle aqueous solution containing 50mM NaCl. React for 6 hours. Subsequently, excessive NaCl was added to make the gold nanoparticles agglomerate and settle, which further promoted the coupling of the gold nanoparti...

Example Embodiment

[0034] Example 2: Add the active intermediate DHLA-NHS and the amino-modified single-stranded primers P15 and P15C to the aqueous solution containing 70% acetonitrile (P15 and P15C are oligonucleotides containing 15 bases and have complementary sequences ), and add a small amount of triethylamine as acid binding agent, react for 12 hours. The DHLA-modified primers P15 and P15C were concentrated using vacuum evaporation equipment, and the acetonitrile component was evaporated.

[0035] Prepare 13nm symmetrical gold nanoparticle dimers connected by 15bp dsDNA. Under equimolar conditions, DHLA-P15 and DHLA-P15C are slowly reduced from 95℃ to room temperature in 1×PBS buffer for paired hybridization into double strands. dsP15, add equimolar dsP15 to a 13nm gold nanoparticle aqueous solution containing 50mM NaCl. React for 6 hours. Subsequently, excessive NaCl was added to make the gold nanoparticles agglomerate and settle to further promote the coupling of gold nanoparticles into d...

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Abstract

The invention relates to a method for preparing a high-purity pitch-adjustable metal nanoparticle dimer at high yield. The method is characterized in that two length-adjustable double-stranded DNA molecules respectively modified by a dual-thiol molecule at the tail ends are used as the connection part; Au (gold) nanoparticles are assembled to a discrete dimer through the chemical bond formed between the thiol and the surface of the Au nanoparticles; and the discrete dimer is separated by one-step electrophoresis method to obtain the high-purity target product. The method provided by the invention can be also used for preparing high-purity Au nanoparticle dimer with symmetric and asymmetric compositions. The method has the advantages of less steps, simple operation, easily-controlled conditions, and good repeatability, and solves the problems of single-DNA-modified Au nanoparticles, such as low assembly purity and complicated steps. The method can provide a firm platform for the basic research of plasmon resonance photonics, and can be used for molecular spectrum studies, such as surface-enhanced Raman scattering and metal-enhanced fluorescence. The prepared high-purity pitch-adjustable metal nanoparticle dimer can be used as an ultrahigh-sensitivity sensor based on plasmon resonance.

Description

technical field [0001] The invention relates to a method for programmably assembling discrete metal nanoparticle plasmon resonance dimers by using biomacromolecular DNA, and belongs to the field of nanomaterial preparation. Background technique [0002] When the incident light of a specific wavelength interacts with the free electrons on the surface of the metal nanoparticles, the collective oscillation of the free electrons can be excited, and the light is confined to the nanoscale on the surface of the metal nanoparticles, that is, localized surface plasmon resonance is generated. Noble metal nanoparticles have unique optoelectronic properties due to the localized surface plasmon resonance effect, such as a significant electromagnetic field enhancement phenomenon in the near-field region of the particle surface, which makes the molecular Raman scattering signal there enhanced by several to a dozen order of magnitude, so as to realize single-molecule Raman spectroscopy dete...

Claims

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Application Information

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IPC IPC(8): B82B3/00B82Y40/00C12Q1/68
Inventor 王强斌兰祥
Owner SUZHOU NAFANG TECH DEV
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