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Mytilus edulis enzymolysis polypeptide and preparation method and application thereof

A mussel enzyme and enzymolysis technology, which is applied in the directions of peptides, hydrolyzed protein components, antitumor drugs, etc., achieves the effects of strong cell proliferation inhibition, simple overall process, and easy popularization and application.

Active Publication Date: 2014-01-15
ZHEJIANG OCEAN UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

But so far there is no report about the effect of mussel enzymatic hydrolyzate on prostate cancer cells

Method used

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  • Mytilus edulis enzymolysis polypeptide and preparation method and application thereof
  • Mytilus edulis enzymolysis polypeptide and preparation method and application thereof
  • Mytilus edulis enzymolysis polypeptide and preparation method and application thereof

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Embodiment Construction

[0029] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0030] 1 material

[0031] 1.1 Animals: Mussels (Mytilus edulis) were purchased from the Zhoushan market (identified by Professor Zhao Shenglong of Zhejiang Ocean University), and after shelling, they were refrigerated at -20°C for later use.

[0032] 1.2 Cell lines: Human prostate cancer cells DU-145 and PC-3 were purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences.

[0033] 1.3 Main reagents: pepsin, trypsin, papain, alkaline protease, dextran gel SephadexG-25 and DEAE SepharoseFF anion exchange columns were purchased from Beijing Asia Pacific Hengxin Biotechnology Co., Ltd.; fetal bovine serum was purchased from Hangzhou Siji Qing Biological Engineering Co., Ltd.; F12 powder medium and MTT were purchased from SIGMA Company of the United States; dimethyl sulfoxide was purchased from AMRESCO Company of the United States;...

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Abstract

The invention discloses a mytilus edulis enzymolysis polypeptide. The mytilus edulis enzymolysis polypeptide is characterized by containing the following amino acid sequence: Asp Leu Tyr. The mytilus edulis enzymolysis polypeptide is prepared by adopting the following steps of: (1) preparing homogenate from mytilus edulis meat, adding alkaline protease, deactivating the protease, centrifuging, and taking clear solution of the upper layer; (2) performing ultra-filtration on the clear solution, collecting hydrolysate with the molecular weight of below 3K, concentrating, and performing freeze drying; (3) performing chromatographic separation by adopting a DEAE-SepharoseFF ion exchange column; (4) performing chromatographic separation by adopting a Sephadex G-25 gel column; and (5) performing high performance liquid chromatography purification. The invention also discloses application of the mytilus edulis enzymolysis polypeptide prepared by the steps in prostatic cancer resistance. Compared with the prior art, the invention has the advantages that: the mytilus edulis is subjected to enzymolysis and purification by adopting an optimal protease and an optimal technology, a strong cell proliferation inhibiting effect is achieved when the obtained target peptide is applied to prostatic cancer resistant cells, and a feasible research path is provided for resisting prostatic cancer.

Description

technical field [0001] The invention relates to a mussel enzymatic polypeptide, and also relates to a preparation method of the mussel enzymatic polypeptide and its application in anti-prostate cancer. Background technique [0002] Polypeptides can be used as active substances such as neurotransmitters, interleukins, and cell growth factors in organisms, and have active functions such as maintaining the growth and development of organisms, immune regulation, and metabolism. Existing studies have shown that peptides derived from organisms have anti-tumor, anti-oxidation, anti-bacterial, anti-aging, anti-hypertensive and other active functions, especially the anti-tumor activity has attracted the attention of researchers from various countries. At present, there have been a large number of reports on the anti-tumor activity of natural peptides and synthetic peptides, and some peptides have been used clinically as anti-tumor drugs; there are many studies on the anti-bacterial, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K2/00C07K1/20C07K1/18C07K1/16A61K38/01A61P35/00
Inventor 丁国芳杨永芳黄芳芳郑玉寅李荣许丹庄黛娜杨最素郁迪徐银峰闫海强
Owner ZHEJIANG OCEAN UNIV
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