External device and method for enriching denitrification anaerobic methane oxidation bacteria by using methane bubbleless aeration biomembrane

Abstract

The invention discloses an external device and a method for enriching denitrification anaerobic methane oxidation bacteria by using a methane bubbleless aeration biomembrane. The device comprises a reactor main body, a methane bubbleless aeration membrane assembly, a temperature control system, a stirring system and a liquid sealing device; the reactor main body is provided with a reactor cover plate, a reaction container, a biological filler, an effluent biomass interception sphere, a circulation liquid biomass interception sphere, and a biological filler support, wherein the reactor main body is divided into a biological filler area and a complete mixing area by a support plate of the biological filler support; the biological filler area comprises the biological filler, the effluent biomass interception sphere, and the circulation liquid biomass interception sphere; the complete mixing area comprises support columns of the biological filler support, a stirring magneton and a water outlet of a circulating liquid water inlet pipe; and a hollow fiber membrane is arranged in the methane bubbleless aeration membrane assembly. The dissolution velocity of methane in an aqueous phase isimproved by a methane bubbleless aeration technology, and the running efficiency of a reactor is improved by a biomembrane technology.

Description

technical field [0001] The invention relates to an external methane bubble-free aeration biofilm denitrification type methane anaerobic oxidizing bacteria enrichment device and method. Background technique [0002] As an important energy source, methane plays an important role in human production and life. At the same time, methane is the most abundant hydrocarbon in the atmosphere, and its contribution to global warming is second only to CO 2 , the current "contribution rate" to global warming reaches 15%, and the greenhouse effect it causes is equivalent to mole CO 2 20 to 30 times of that. It is reported that 85% of the world's annual methane production and 60% of its consumption are based on the action of microorganisms. Anaerobic oxidation of methane by microorganisms can make most of the methane gas (more than 90%) be consumed in large quantities before entering the atmosphere. Therefore, anaerobic methane oxidation plays a role that cannot be ignored in the global...

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Problems solved by technology

[0008] The denitrification-type anaerobic oxidation of methane is usually limited to regions characterized by sharp gradients, so that the reaction occurs only in the range of a few millimeters, making the process difficult to detect

Moreover, the growth of functional microorganisms for denitrifying anaerobic methane oxidation is extremely slow, and the cell doubling time is as long as more than one month, so it is difficult to obtain an enriched culture that catalyzes this process

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