Application of sinomenine in preparation process of drugs inhibiting invasion and metastasis of tumors
A technology for sinomenine and tumors, which is applied in the application field of sinomenine in the preparation of drugs for inhibiting tumor invasion and metastasis, and can solve the problems that there are no relevant reports on the inhibition of tumor invasion and metastasis by sinomenine, and achieve wide medicinal value, Effects with low toxicity and few side effects
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[0032] Example 1:
[0033] Human liver cancer cell line MHCC97H, human pancreatic cancer cell line PANC-1, human colon cancer cell line HT-29 and human gastric adenocarcinoma cell line HGC-27 all confirmed the inhibitory effect of sinomenine on tumor invasion and metastasis by the following methods:
[0034] 1. MTT method to detect cell proliferation:
[0035] The four types of tumor cells of the digestive system in the logarithmic growth phase were digested with 0.25% trypsin, the cells were counted, and the single cell suspension was diluted with high-glycemic DMEM medium to the required cell concentration of 5×104 / ml, 100μl per well, Seed in 96-well cell plate, 37℃, 5% CO 2 Incubate overnight; according to the report, carry out sample dilution, add 100μl of sample diluted in serum-free high-glycemic DMEM medium to each well, and set up blank control group, solvent control group and zero adjustment hole at the same time, each group has 6 multiple holes, 37 ℃, Continue culturing w...
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