Extraction method for costunolide

The technology of a woody lactone and an extraction method, which is applied in the field of extracting effective parts, can solve the problems such as the unpublished method for extracting the woody lactone, and achieve the effects of easy availability of raw materials, reasonable method and simple operation.

Inactive Publication Date: 2012-07-18
HUAIHAI INST OF TECH
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0009] In the prior art, there is no disclosure of a method for extracting woody lactones from the root bark of Zuixiang Michelia as a raw material

Method used

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  • Extraction method for costunolide
  • Extraction method for costunolide
  • Extraction method for costunolide

Examples

Experimental program
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Effect test

Embodiment 1

[0021] Example 1. A method for extracting costanolactone, the steps are as follows:

[0022] (1) Take the root bark of Zuixiang Michelangelo as a raw material, dry and crush; then use 95% ethanol to reflux extract 2-4 times, combine the extracts and concentrate under reduced pressure to obtain a crude extract;

[0023] (2) Take an appropriate amount of the dried above crude extract and dissolve it with ethyl acetate to form a solution for thin layer chromatography; use 10:1 petroleum ether-ethyl acetate as a developing solvent, perform thin layer chromatography, and use iodine to show Color, calculated R of costanolactone f = 0.2;

[0024] (3) Take 1.4743 g of the above-mentioned crude extract that has been dried, and perform atmospheric silica gel column chromatography, successively carry out gradient elution with 10:1, 5:1 petroleum ether-ethyl acetate as the eluent, and detect by TLC In the receiving solution of each test tube, the substance in the first spot is eluted from the ...

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Abstract

An extraction method for costunolide comprises the following steps of choosing micheliamacclurel velamen as a raw material, drying and smashing; extracting 2 to 4 times with 95% of ethanol in reflux mode, combining extraction liquid and decompressing and concentrating to obtain coarse extractum; taking the dried coarse extractum to perform normal-pressure silica gel column chromatography, sequentially choosing petroleum ether - ethyl acetate of 10:1 and 5:1 as eluant to perform gradient elute, using thin-layer chromatography (TLC) to detect received liquid in all test tubes, adopting the elutant of 10:1 to elute a first spot material clean from a chromatography column, using the elutant of 5:1 to continue eluting, receiving a second spot material, confirming that no spot exists through TLC detection, finishing the column chromatography, combining the received liquid, and concentrating the received liquid to obtain white flaky crystal, namely the costunolide. The extraction method uses the micheliamacclurel velamen as the raw material to prepare monomer costunolide and is reasonable, convenient in operation, easy to obtain the raw material and capable of manufacturing costunolide monomers.

Description

Technical field [0001] The invention relates to a method for extracting effective parts from raw materials of Chinese herbal medicine, in particular to a method for extracting costanolactone. Background technique [0002] Costunolide (costunolide), also known as costunolide, its molecular formula is C 15 H 20 O 2 , Molecular weight 232.3181, CAS number 553-21-9. Its structural formula is as follows: [0003] [0004] The Chinese Open Patent Document with the application number 200710119450 discloses a method for preparing costanolactone, which is a method for extracting costanolactone from the leaves of Magnolia radiata. The extraction steps are as follows: [0005] ①Water extraction and impurity disposal: The crude Magnolia leaf powder is soaked and extracted twice in deionized water for 12 hours each time, filtered, and the water extract is discarded, and the residue is used for later use; [0006] ②Preparation of crude extract of costanolactone: The leaves of Magnolia grandiflora...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D307/93
Inventor 宋晓凯
Owner HUAIHAI INST OF TECH
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