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Method for preparing bioflocculant through high-concentration fermentation

A bioflocculant and high-concentration technology, applied in the field of bioengineering, can solve the problems of limited promotion and application, low production efficiency, and low product concentration, and achieve the effects of low production cost, high product activity, and high fermentation yield

Active Publication Date: 2012-07-18
QINGDAO YAODONG GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in actual application, this technology has the disadvantages of low product concentration (product output ≤ 0.76% (w / v)), low production efficiency, cumbersome operation, and high cost, which limits widespread promotion and application.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) Expanded cultivation of production strains

[0031] ①Slant medium and slant strain culture: beef extract 4g / L, soybean peptone 10g / L, liquid sugar 7g / L, NaCl 5g / L, pH7.0-7.2; micrococcus DS16 was inoculated on the fresh slant, and Incubate at 28-30°C for 36h.

[0032] ② Shake flask medium and shake flask strain culture: liquid sugar 8g / L, soybean peptone 9g / L, yeast extract 0.3g / L, K2 HPO 4 4g / L, KH 2 PO 4 3g / L, MgSO 4 ·7H 2 O 0.4g / L, pH7.2; each 300mL Erlenmeyer flask was filled with 70mL of liquid shaker flask culture medium, and each bottle was inoculated with an inoculation loop of slant bacteria, 180r / min, 28-30℃ for 9h.

[0033] ③ production strain culture medium and production strain culture: production strain culture medium is the same as shake flask culture medium; insert shake flask strain by 2.5% volume ratio and carry out ventilation culture, sterile air ventilation ratio 1: 0.4 (V / V), the stirring speed is 180r / min, cultivated at 27-30°C for 10h,...

Embodiment 2

[0044] (1) Expanded cultivation of production strains

[0045] ①Slant culture medium and slant strain culture: beef extract 3.5g / L, soybean peptone 9g / L, liquid sugar 8g / L, NaCl 4g / L, pH7.0-7.2; Micrococcus DS16 was inoculated on the fresh slant, and Incubate at 28-30°C for 36h.

[0046] ② Shake flask medium and shake flask strain culture: liquid sugar 9g / L, soybean peptone 8g / L, yeast extract 0.4g / L, K 2 HPO 4 5g / L, KH 2 PO 4 2.5g / L, MgSO 4 ·7H 2 O 0.35g / L, pH7.2; each 300mL Erlenmeyer flask is filled with 70mL of liquid shaker flask medium, and each bottle is inoculated with an inoculation loop of slant strains, 180r / min, 28-30℃ for 8-12h.

[0047] 3. production strain culture medium and production strain culture: production strain culture medium is the same as shake flask culture medium; Insert the shake flask strain of invention by 3% volume ratio and carry out ventilation culture, sterile air ventilation ratio 1: 0.5 ( V / V), the stirring speed is 160r / min, and cult...

Embodiment 3

[0058] (1) Expanded cultivation of production strains

[0059] ①Slant medium and slant strain culture: beef extract 4.2g / L, soybean peptone 8.5g / L, liquid sugar 9g / L, NaCl4.5g / L, pH7.0-72; micrococcus DS16 was inoculated on the fresh slant , and incubated at 28-30°C for 36h.

[0060] ② Shake flask medium and shake flask strain culture: liquid sugar 10g / L, soybean peptone 9.5g / L, yeast extract 0.35g / L, K 2 HPO 4 5.5g / L, KH 2 PO 4 2g / L, MgSO 4 ·7H 2 O 0.5g / L, pH7.2; each 300mL Erlenmeyer flask is filled with 70mL of liquid shaker flask medium, and each bottle is inoculated with an inoculation loop of slant strains, 180r / min, 28-30℃ for 8-12h.

[0061] 3. production strain culture medium and production strain culture: production strain culture medium is the same as shake flask culture medium; Insert the shake flask strain of invention by 3.5% volume ratio and carry out ventilation culture, sterile air ventilation ratio 1: 0.55 ( V / V), the stirring speed is 180r / min, and cu...

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PUM

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Abstract

The invention discloses a method for preparing a bioflocculant through high-concentration fermentation, belonging to the field of bioengineering. The bioflocculant for purifying water quality is produced through high-concentration fermentation in a bacterial quantity dominance way by selecting micrococcus DS16 as a seed lot of the bioflocculant. The preparation process comprises the following steps of seed lot preparation, fermentation liquid preparation, fermentation liquid pretreatment and product extraction. The prepared bioflocculant is non-toxic and innocuous, has biodegradability and no secondary pollution when being used and can be safely and efficiently applied to the separation and the removal of heavy metals and other pollutants in polluted waste water. The method for preparing the bioflocculant through high-concentration fermentation, provided by the invention, has the characteristics of low production cost, capability of carrying out large-scale production, high fermentation yield (larger than or equal to 2.0%), high product activity (700g of pollutants can be separated and removed from water by using each gram of powdery product), and the like.

Description

technical field [0001] The invention relates to a preparation method of a biological flocculant with flocculation and adsorption functions, in particular to a method for producing a biological flocculant for water purification treatment through high-concentration fermentation, which belongs to the field of biological engineering. Background technique [0002] At present, with the rapid development of industry and the rapid increase of population, the demand for urban drinking surface water and the discharge of industrial wastewater, urban domestic sewage, and aquaculture wastewater are increasing day by day. Due to the continuous increase in the types of sewage discharge, the pollutant composition in water has become more and more complex, so it is more and more difficult to treat sewage. If not handled properly, it will pose a serious threat to industrial and agricultural production and human health. The use of flocculants to flocculate pollutants is an effective means to ...

Claims

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Application Information

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IPC IPC(8): C12P1/06C02F1/56C12R1/265
Inventor 栾兴社
Owner QINGDAO YAODONG GRP