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Preparation method of silymarin

A technology of silymarin and culture medium, applied in the field of preparation of silymarin, can solve the problems of environmental pollution, solvent residues in products, low extraction rate and the like

Inactive Publication Date: 2012-07-18
佳木斯三江水飞蓟素制品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] my country is a big country for planting milk thistle, but the current production and utilization of silymarin is limited to the research of crude product extraction process, the product yield is low, and the purity is relatively low, resulting in low added value of the product
The results of chemical analysis show that silymarin mainly exists in the pericarp, but most of the current extraction methods of silymarin are limited to organic solvent extraction such as acetone. This method can only extract the free silymarin in the pericarp of milk thistle, and the extraction rate is low. The product has solvent residues, which will cause certain environmental pollution during processing

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The two strains used in the present invention were purchased from the China Industrial Microorganism Culture Collection and Management Center, respectively: Trichoderma reesei CICC13052 and Trichoderma korning CICC 40144.

[0038] 1. Preparation process of compound microbial starter freeze-dried powder

[0039] 1.1 Activation of the original strain

[0040] Measure 10ml of 0.9% normal saline into 2 test tubes, sterilize at 121°C for 20 minutes and cool to 28°C, pour all the freeze-dried bacteria powders in the ampoules of 2 strains into 0.9% In physiological saline, oscillate to make it dissolve, and activate it in a 28°C incubator for 30 minutes, and set aside.

[0041] 1.2 Expanded culture of fermentation bacteria

[0042] 1.2.1 Preparation of mother starter

[0043] Measure 200ml of culture medium for each of the two strains into two 500ml Erlenmeyer flasks, sterilize at 121°C for 20 minutes and cool to 28°C, inoculate 10% of the volume of the culture medium with ...

Embodiment 2

[0062] 1. Preparation process of compound microbial starter freeze-dried powder

[0063] 1.1 Activation of the original strain

[0064] Measure 10ml of 0.9% normal saline into 2 test tubes, sterilize at 121°C for 20 minutes and cool to 28°C, pour all the freeze-dried bacteria powders in the ampoules of 2 strains into 0.9% In physiological saline, oscillate to make it dissolve, and activate it in a 28°C incubator for 30 minutes, and set aside.

[0065] 1.2 Expanded culture of fermentation bacteria

[0066] 1.2.1 Preparation of mother starter

[0067] Measure 200ml of culture medium for each of the two strains into two 500ml Erlenmeyer flasks, sterilize at 121°C for 20 minutes and cool to 28°C, inoculate 10% of the volume of the culture medium with the activated strains in step 1.1, at 28°C, The shaking table was cultivated for 24 hours under the condition of the shaking table rotating speed of 150 rpm, as the mother starter.

[0068] 1.2.2 Preparation of production starter ...

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Abstract

The invention provides a preparation method of silymarin. The preparation method comprises the steps of: firstly preparing composite microorganism fermenting agent freeze-dried powder of Trichoderma reesei CICC 13052 and Trichoderma koningii CICC 40144, activating with 2% glucose solution, inoculating the activated composite microorganism fermenting agent freeze-dried powder to silybum mariamum deoiled seed cake powder with moisture content of 40-45% and sucrose content of 4.2-4.8%, carrying out heat-insulation fermentation at 25-28 DEG C for 60h, drying by hot air, extracting at 15-20 DEG C and vacuum degree of -0.1MPa for 10h to obtain yellow powdered substance, adding methanol for dissolving, filtering, and vacuum-drying insoluble substance to obtain silymarin.

Description

(1) Technical field [0001] The invention relates to a preparation method of silymarin. (2) Background technology [0002] Milk thistle is a plant of the genus Milk thistle in the family Asteraceae. It was introduced into my country in 1972 and promoted as a medicinal plant for protecting the liver. Silymarin is the active ingredient extracted and refined from the dried fruit of milk thistle, and its medicinal ingredient is the total flavonoids of milk thistle. Silymarin has pharmacological activities of anti-hepatotoxicity, protection of liver cell membrane, and improvement of liver function. It is clinically used to treat hepatitis, cholecystitis, etc. In recent years, studies have found that silymarin not only has the effect of protecting the liver and gallbladder, but also has obvious effects of anti-lipid peroxidation, anti-radiation, scavenging free radicals and anti-gastric ulcer, and has low toxicity, no teratogenic and mutagenic effects, It has good effects in redu...

Claims

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Application Information

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IPC IPC(8): C12P17/16C07D407/04C12R1/885
Inventor 张贵彭波杨喜春
Owner 佳木斯三江水飞蓟素制品有限公司
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