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Method for repairing high-concentration oil-polluted soil through mycorrhizal fungi-plant-degradation bacterium

A technology for oil pollution and mycorrhizal fungi, applied in the field of contaminated soil remediation, can solve the problems of slow plant growth, low degradation efficiency and low cost, and achieve the effects of promoting plant growth, improving stress resistance and increasing exposure

Inactive Publication Date: 2012-07-25
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The bioremediation technology has the most optimistic application prospects, can be treated in situ, and has low cost and no secondary pollution. However, bioremediation methods such as single microorganisms, plants or plant-microbes are all because organisms cannot tolerate high-pollution environments. The phenomenon of slow reproduction or plant growth leads to the problem of low degradation efficiency in a short period of time; then, the ability of organisms to tolerate pollutants and to survive and grow quickly in a polluted environment becomes the basis for bioremediation of polluted environments

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0020] Example 1: Take 2.0 kg of petroleum-contaminated soil with a petroleum hydrocarbon content of 1998.6 mg / kg and put it in a flowerpot, mix in 60 g of Glomus mosseae bacterial agent, adjust the soil moisture content to 50%, and let it stand overnight. Clover and corn seeds treated with 10% H 2 o 2 After surface disinfection and germination, sow in the soil inoculated with mycorrhizal mycorrhizal fungi. The sowing rate is 15 clover and 5 corn / pot. Thin the seedlings when they germinate to the three-leaf stage, leaving 8 clover in each pot. Two corn plants were inoculated with petroleum hydrocarbon-degrading bacteria at the rhizosphere at the same time. The dosage of the bacteria agent was 200ml per pot, and then the management method of ordinary crops was adopted; soil and corn plant samples were collected for analysis after 124 days to evaluate the restoration effect.

[0021] The research results showed that after 124 days of cultivation, compared with the control treat...

example 2

[0022] Example 2: Take 2.0 kg of petroleum-contaminated soil with a petroleum hydrocarbon content of 1998.6 mg / kg and put it in a flowerpot, mix in 60 g of Glomus versifome bacterial agent, adjust the soil moisture content to 50%, and let it stand overnight. Clover and corn seeds treated with 10% H 2 o 2 After surface disinfection and germination, sow in the soil inoculated with mycorrhizal mycorrhizal fungi. The sowing rate is 15 clover and 5 corn / pot. Thin the seedlings when they germinate to the three-leaf stage, leaving 8 clover in each pot. Two corn plants were inoculated with petroleum hydrocarbon-degrading bacteria at the rhizosphere at the same time. The dosage of the bacteria agent was 200ml per pot, and then the management method of ordinary crops was adopted; soil and corn plant samples were collected for analysis after 124 days to evaluate the restoration effect.

[0023] The research results showed that after 124 days of cultivation, compared with the control tre...

example 3

[0024] Example 3: Take two parts of oil-contaminated soils with a petroleum hydrocarbon content of 9860mg / kg, each 2.0kg, and put them in two flower pots respectively, and mix them with Glomus mosseae and Glomus versifome bacteria 60g of potion, adjust the moisture content of the soil to 50%, and let it stand overnight. Clover and corn seeds treated with 10% H 2 o 2 After surface disinfection and accelerated germination, sow in the soil that has been inoculated with mycorrhizal mycorrhizal fungi. The sowing amount per pot is 15 clover and 5 corn. Thin the seedlings when they germinate to the three-leaf stage, leaving 8 clover in each pot , 2 corn plants were inoculated with petroleum hydrocarbon degrading bacteria in the rhizosphere of the plants at the same time. The dosage of the bacteria was 200ml per pot, and then the management method of ordinary crops was adopted; soil and corn plant samples were collected for analysis after 124 days to evaluate the restoration effect. ...

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Abstract

The invention discloses a method for repairing high-concentration oil-polluted soil through mycorrhizal fungi-plant-degradation bacterium, concretely comprising the the steps of: inoculating an arbuscular mycorrhizal fungi inoculant such as Glomus mosseae bacteria or Glomus versiforme bacteria and a mixed inoculant of petroleum hydrocarbon and degradation bacterium liquid in the high-concentration oil-polluted soil and planting mycorrhizal fungi aulophytes such as shamrocks and corns, growing for 90-125 days, and harvesting corn plant bodies with high biomass liveweight and burning in a centralized manner. The method has the advantage of establishing a composite mycorrhizal fungi-plant-degradation bacterium co-metabolism system; on one side, the mycorrhizal fungi and plants form mutually beneficial symbionts so as to help the plants to absorb nutrition and water and promote the growth of the plants so that the stress resistance of the plants to the high-concentration oil-polluted soil; on the other side, the mycorrhizal fungi is used as heterotrophic microorganism and the petroleum hydrocarbon is used as exogenous carbon which is decomposed and utilized; and moreover, nutrients are provided to exogenous petroleum hydrocarbon degradation bacteria through mycorrhizal fungi and the secretions of plant root cells so that the degradation efficiency of the petroleum hydrocarbon is improved.

Description

technical field [0001] The invention relates to the technical field of contaminated soil remediation, in particular to a method for remediating high-concentration petroleum-contaminated soil by mycorrhizal fungi-plant-degrading bacteria. Background technique [0002] China is a big country in oil production and consumption in the world, but it is followed by increasingly serious soil oil pollution. Due to reasons such as storage and transportation of petroleum, petroleum smelting, leakage accidents, and irrigation of oily sewage, petroleum-contaminated soils are increasing year by year; it is reported that about 6×10 5 tPetroleum enters the environment through running, escaping, dripping, leaking, etc., causing soil pollution; in addition, the polluted area of ​​each oil production well is as high as 500m2 2 , and the area of ​​crude oil pollution in oil-producing areas is still expanding year by year. In some heavily polluted areas of oil fields, the soil crude oil content...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/00B09C1/10
Inventor 郭光霞朱永官乔敏
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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