Method for repairing high-concentration oil-polluted soil through mycorrhizal fungi-plant-degradation bacterium

Abstract

The invention discloses a method for repairing high-concentration oil-polluted soil through mycorrhizal fungi-plant-degradation bacterium, concretely comprising the the steps of: inoculating an arbuscular mycorrhizal fungi inoculant such as Glomus mosseae bacteria or Glomus versiforme bacteria and a mixed inoculant of petroleum hydrocarbon and degradation bacterium liquid in the high-concentration oil-polluted soil and planting mycorrhizal fungi aulophytes such as shamrocks and corns, growing for 90-125 days, and harvesting corn plant bodies with high biomass liveweight and burning in a centralized manner. The method has the advantage of establishing a composite mycorrhizal fungi-plant-degradation bacterium co-metabolism system; on one side, the mycorrhizal fungi and plants form mutually beneficial symbionts so as to help the plants to absorb nutrition and water and promote the growth of the plants so that the stress resistance of the plants to the high-concentration oil-polluted soil; on the other side, the mycorrhizal fungi is used as heterotrophic microorganism and the petroleum hydrocarbon is used as exogenous carbon which is decomposed and utilized; and moreover, nutrients are provided to exogenous petroleum hydrocarbon degradation bacteria through mycorrhizal fungi and the secretions of plant root cells so that the degradation efficiency of the petroleum hydrocarbon is improved.

Description

technical field [0001] The invention relates to the technical field of contaminated soil remediation, in particular to a method for remediating high-concentration petroleum-contaminated soil by mycorrhizal fungi-plant-degrading bacteria. Background technique [0002] China is a big country in oil production and consumption in the world, but it is followed by increasingly serious soil oil pollution. Due to reasons such as storage and transportation of petroleum, petroleum smelting, leakage accidents, and irrigation of oily sewage, petroleum-contaminated soils are increasing year by year; it is reported that about 6×10 5 tPetroleum enters the environment through running, escaping, dripping, leaking, etc., causing soil pollution; in addition, the polluted area of ​​each oil production well is as high as 500m2 2 , and the area of ​​crude oil pollution in oil-producing areas is still expanding year by year. In some heavily polluted areas of oil fields, the soil crude oil content...

AI Technical Summary

Problems solved by technology

The bioremediation technology has the most optimistic application prospects, can be treated in situ, and has low cost and no secondary pollution. However, bioremediation methods such as single microorganisms, plants or plant-microbes are all because organisms cannot tolerate high-pollution environments. The phenomenon of slow reproduction or plant growth leads to the problem of low degradation efficiency in a short period of time; then, the ability of organisms to tolerate pollutants and to survive and grow quickly in a polluted environment becomes the basis for bioremediation of polluted environments