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Method for extracting yeast glucosan by utilizing enzymatic method

A technology of glucan and yeast, which is applied in the field of comprehensive utilization of beer waste yeast, can solve the problems of high energy consumption in the extraction process, high requirements for acid and alkali resistance, and high environmental protection pressure, and achieve the effects of improving solubility, low emissions, and low energy consumption

Inactive Publication Date: 2013-08-21
无锡德冠生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At home and abroad, the yeast glucan is mainly separated and purified by the acid-base method of beer yeast dregs. Although yeast β-1,3-glucan can be obtained, the extraction process requires high energy consumption, high requirements for acid and alkali resistance of equipment, and great pressure on environmental protection.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Take 100 grams of dry brewer's yeast residue, add 1000 ml of water, stir evenly, heat to 95°C, add 50,000 units of α-amylase, and keep for 30 minutes; cool down to 55°C, add 100,000 units of saccharification enzyme and 50,000 units of mannan Carbohydrase, incubate for 1.5 hours; add 100,000 units of neutral protease, incubate for 1 hour, and collect the precipitate by centrifugation; add 1000 ml of water to the precipitate, heat it to 95°C, add 30 g of sodium hydroxide, react for 1 hour, and collect the precipitate by centrifugation ; Add 1000 ml of water to the precipitate, stir the water to elute the salt, repeat the washing step twice, and collect the precipitate by centrifugation; stir the precipitate with ethanol, leaching, degreasing, and dehydrating three times, and finally drying in an oven to obtain yeast β-1,3- 8.5 grams of glucan, the purity of yeast beta-1,3-glucan is 90.4%.

Embodiment 2

[0015] Add 750 liters of water and 50 kg of brewer's yeast residue to a 1000-liter reaction tank, stir evenly, heat up to 90 °C, add α-amylase according to the ratio, and keep it for 60 minutes; cool down to 65 °C, add saccharification enzyme and mannanase according to the ratio , incubated for 1.5 hours; neutral protease was added according to the proportion, reacted for 1.5 hours, and the precipitate was collected by centrifugation; the precipitate was washed with water to elute the salt, and the washing step was repeated twice, and the precipitate was collected by centrifugation; The dryer was spray-dried, the air inlet temperature was 180°C, and the air outlet temperature was 85-90°C to obtain 4.2 kg of light white yeast beta-1,3-glucan powder with a particle size larger than 300 meshes. The yeast beta-1,3-glucan was 91.2% pure.

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Abstract

The invention relates to a method for extracting yeast glucosan by utilizing an enzymatic method, belonging to the technical field of comprehensive utilization of waste beer yeast. According to the invention, high-purity yeast beta-1,3-glucosan is prepared by use of beer yeast slag as the by-product in the production process of beer. The method comprises the following steps: (1) dispersing the beer yeast slag in water so that the mass ratio of the material to water is 1:10 to 1:20 and stirring completely; (2) adding alpha-amylase in the beer yeast slag suspension at 90-95 DEG C, and stirring to allow complete reaction; (3) cooling to 50-70 DEG C, regulating the pH value to 4-6, adding a saccharifying enzyme and mannase, and stirring to allow complete reaction; (4) adding neutral protease in the suspension, stirring, allowing complete reaction and centrifuging to collect the precipitate; and (5) adding water in the precipitate, washing to desalt, centrifuging and drying to obtain the product. The prepared yeast beta-1,3-glucosan has the advantage of high purity, the preparation process is simple, equipment requirements and the cost are low, and the method is suitable for industrialproduction.

Description

technical field [0001] The invention relates to a method for extracting yeast glucan by an enzymatic method, in particular to a preparation method of yeast beta-1,3-glucan, which belongs to the technical field of comprehensive utilization of waste beer yeast. Background technique [0002] Yeast β-1,3-glucan is one of the main constituents of yeast cell wall, and widely exists in baker's yeast and brewer's yeast. In addition to enhancing yeast β-1,3-glucan is a microbial natural active polysaccharide, as a functional factor, it is mainly used to enhance human immunity, by inducing the body to produce immune responses to defend against bacteria, fungi, viruses, etc. It has been shown that yeast glucan can also effectively inhibit viruses such as SARS and anthrax, and is a good immune function activator in the human body. In addition to its use in human immunity, it can also be used to inhibit tumor growth and lower cholesterol. Waste beer yeast is the main by-product of the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/02
Inventor 郁磊陶丁伟邹联
Owner 无锡德冠生物科技有限公司