Method for preparing chlorsulfuron molecular imprinting SPR (surface palsmon resonance) sensor chip

A technology of chlorsulfuron molecule and sensor chip, which is applied to the measurement of phase influence characteristics, etc., can solve the problems of poor sensitivity of the chip, and achieve the effects of fast response speed, simple preparation method and low cost

Inactive Publication Date: 2012-07-25
INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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Problems solved by technology

[0007] The present invention aims to solve the technical problem of poor sensitivity of chips prepared by existing molec...

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  • Method for preparing chlorsulfuron molecular imprinting SPR (surface palsmon resonance) sensor chip
  • Method for preparing chlorsulfuron molecular imprinting SPR (surface palsmon resonance) sensor chip
  • Method for preparing chlorsulfuron molecular imprinting SPR (surface palsmon resonance) sensor chip

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specific Embodiment approach 1

[0018] Embodiment 1: The preparation method of the chlorsulfuron molecularly imprinted SPR sensor chip of this embodiment is carried out according to the following steps:

[0019] 1. Coating: adopt the method of vacuum evaporation coating, first deposit chromium with a thickness of 1.5nm to 2.5nm on the glass sheet, and then deposit gold with a thickness of 45nm to 55nm and a purity of 99.9999%, to obtain a coated glass sheet;

[0020] Two, cleaning: the mass percentage concentration is 30% H 2 o 2 With a mass percentage concentration of 98% H 2 SO 4 The volume ratio is 1:3 and mixed evenly to obtain the lotion; then the coated glass piece obtained in step 1 is washed with the lotion, then cleaned with deionized water, finally cleaned with absolute ethanol, and dried to obtain the chip base;

[0021] 3. Preparation of chlorsulfuron molecularly imprinted polymer nanospheres: Weigh chlorsulfuron, diethylaminoethyl methacrylate (DEMAA), trimethylolpropane trimethacrylate (TRI...

specific Embodiment approach 2

[0024] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the template removal agent described in step 3 is a methanol solution of 10% by mass of acetic acid. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0025] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that the method for removing chlorsulfuron-methyl with a template remover described in step 3 is the Soxhlet extractor method. Others are the same as in the first embodiment.

[0026] The Soxhlet extractor method is a conventional method of operation.

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Abstract

The invention discloses a method for preparing a chlorsulfuron molecular imprinting SPR (surface palsmon resonance) sensor chip, and relates to a method for preparing an SPR sensor chip. The invention aims at solving the technical problem of poor sensitivity of a chip prepared by the existing molecular imprinting polymer membrane. The method comprises the following steps: depositing chrome and gold on a glass sheet and washing to obtain a chip substrate; adding chlorsulfuron, N,N-diethylaminoethyl methacrylate, trihydroxymethylpropyl trimethylacrylate and 2,2'-azobis(2-methylpropionitrile) into acetonitrile to perform a reaction, removing the chlorsulfuron template in reaction products, and drying to obtain chlorsulfuron molecular imprinting polymer nanospheres; adding chlorsulfuron molecular imprinting polymer nanospheres, polyvinyl chloride and dibutyl phthalate into tetrahydrofuran and mixing evenly to obtain a coating liquid; and coating the coating liquid onto the chip substrate to obtain the chlorsulfuron molecular imprinting SPR sensor chip. The detection limit of the chip to chlorsulfuron can reach 20ng/mL, can detect trace amount of chlorsulfuron and be can used in the technical fields of sensing and separation.

Description

technical field [0001] The invention relates to a preparation method of an SPR sensor chip. Background technique [0002] Chlorsulfuron is a high-efficiency, broad-spectrum, and highly selective herbicide. At present, it is widely used in oil crops such as soybeans and corn. This kind of herbicide is generally used in a small dose, but because it is a long-lasting herbicide, the trace residue in the soil will cause phytotoxicity to many sensitive crops and cause huge losses to farmers. Countries have formulated residue limits successively. Most countries stipulate the MRL of similar drugs: between 0.05-2ppm. Due to its unstable nature and low residue, it is more difficult to monitor. [0003] At present, the main method for analyzing the residues of sulfonylurea herbicides is liquid chromatography and its combined technique. However, due to its low residual concentration, cumbersome pretreatment and large matrix interference, the effective detection of chlorsulfuron resid...

Claims

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Application Information

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IPC IPC(8): G01N21/41
Inventor 王静佘永新王淼史晓梅
Owner INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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