Method for ultrasound-assisted extraction of lappaconitine

A technology of ultrasonic-assisted extraction and high urine, applied in the direction of organic chemistry, can solve the problems of cumbersome process, high cost, cumbersome operation process, etc., and achieve the effect of easy industrialization, short production cycle and simple process

Inactive Publication Date: 2012-08-01
NORTHWEST NORMAL UNIVERSITY
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  • Abstract
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Problems solved by technology

The extract or eluent is concentrated, diluted with ethanol, left standing, and separated to obtain the total biological alkaloids, and the total biological alkaloids are recrystallized with ethanol to obtain the refined product of labaconitine. This method uses the toxic solvent chloroform, and the obtained product There is solvent residue in; CN101967124A discloses "a kind of method for separating and purifying homogenin". After the desalination treatment, the separation and purification method of homogenin through neutral alumina column separation, acetone-ethanol (1:1) recrystallization and other methods in one, the yield of homogenin is 0.57%, and the purity reaches 98.16%, but The process of this method is still relatively cumbersome and costly
[0005] In summary, there are several problems in the reported extraction methods of quinine: (1) the use of toxic and carcinogenic organic solvents such as chloroform or benzene for extraction, the product has solvent residues, and the safety is poor; (2) the operation process is relatively cumbersome , time-consuming, high cost and low extraction efficiency
[0006] Ultrasonic-assisted extraction is a green, environmentally friendly, efficient and energy-saving extraction method that uses the high-speed, strong cavitation effect and stirring effect generated by ultrasonic waves to destroy the cells of medicinal materials, allowing the solvent to penetrate into the cells of medicinal materials and extract active ingredients. Due to the advantages of short time and high extraction efficiency, the method of ultrasonic-assisted extraction of aconitin in aconitum has not been reported in the literature so far

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  • Method for ultrasound-assisted extraction of lappaconitine

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Embodiment 1

[0042] A kind of method of ultrasound-assisted extraction of homogenate of the present invention, comprises the following steps:

[0043] 1) Medicinal material pretreatment

[0044] Pulverize the roots of Aconitum aconitum and pass through a 60-mesh sieve to obtain medicinal powder of Aconitum aconitum;

[0045] 2) Ultrasonic extraction

[0046] The high aconitum medicinal powder and the ethanol that the volume fraction is 95% are soaked for 12 hours according to the mass volume ratio of 1g: 10mL, using an ultrasonic cleaner (KQ-250DE type numerical control ultrasonic cleaner, Kunshan Ultrasonic Instrument Co., Ltd.), at a power of 150W , under the condition of a temperature of 40° C., ultrasonic extraction for 30 minutes; suction filtration under the condition of a vacuum of 0.07 MPa, repeating step 2) for the obtained filter residue to extract once more, and combining the two filtrates for suction filtration to obtain the filtrate;

[0047] 3) Preparation of acid water

[0...

Embodiment 2

[0054] A kind of method of ultrasound-assisted extraction of homogenate of the present invention, comprises the following steps:

[0055] 1) Medicinal material pretreatment

[0056] Pulverize the roots of Aconitum aconitum and pass through an 80-mesh sieve to obtain medicinal powder of Aconitum aconitum;

[0057] 2) Ultrasonic extraction

[0058] The high aconitum medicinal powder and the ethanol whose volume fraction is 95% are soaked for 36 hours according to the mass volume ratio of 1g: 20mL, using an ultrasonic cleaner (KQ-250DE type numerical control ultrasonic cleaner, Kunshan Ultrasonic Instrument Co., Ltd.), at a power of 250W , under the condition of a temperature of 60° C., ultrasonic extraction for 40 minutes; suction filtration under the condition of a vacuum of 0.08 MPa, repeat step 2) of the obtained filter residue to extract once more, and combine the filtrates twice to obtain the filtrate;

[0059] 3) Preparation of acid water

[0060] The filtrate obtained ...

Embodiment 3

[0069] A kind of method of ultrasound-assisted extraction of homogenate of the present invention, comprises the following steps:

[0070] 1) Medicinal material pretreatment

[0071] Pulverize the roots of Aconitum aconitum and pass through a 60-mesh sieve to obtain medicinal powder of Aconitum aconitum;

[0072] 2) Ultrasonic extraction

[0073] The high aconitum medicament powder and the ethanol that the volume fraction is 95% are soaked 24 hours according to mass volume ratio 1g: 15mL, use ultrasonic cleaner (KQ-250DE type digital control ultrasonic cleaner, Kunshan Ultrasonic Instrument Co., Ltd.), in power 200W , under the condition of a temperature of 50° C., ultrasonic extraction for 50 minutes; suction filtration under the condition of a vacuum of 0.09 MPa, repeat step 2) for the obtained filter residue to extract once more, and combine the filtrates twice to obtain the filtrate;

[0074] 3) Preparation of acid water

[0075] The filtrate obtained in step 2) was conc...

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Abstract

The invention discloses a method for ultrasound-assisted extraction of lappaconitine, belonging to the technical field of preparation of traditional Chinese medicine. The preparation method comprises the following steps of: (1) herb pretreatment; 2) ultrasound extraction; 3) preparation of acidic solution; (4) extraction; and (5) crystallization. The lappaconitine is extracted from aconitum sinomontanum via high-speed and intense cavitation and mixing effects produced by ultrasonic waves. The method has the advantages of simple process, convenience in operation, high efficiency, energy conservation, short production cycle, low cost, high yield and easiness in industrialization, no toxic solvents such as chloroform and benzene are used in the operating process, the method is environment-friendly, and the lappaconitine cannot be polluted.

Description

technical field [0001] The invention relates to a method for extracting homogenin, in particular to a method for ultrasonically assisted extraction of homogenin. Background technique [0002] Aconitum Sinomontanum Nakai is a perennial herb of the genus Aconitum in the Ranunculaceae family. It is mainly distributed in Sichuan, Gansu, Qinghai, Shaanxi, Shanxi, and Hebei. It is a good source plant for analgesic drugs. [0003] Gaowujiasu is a diterpene alkaloid extracted from the root of Aconitum aconitum, which has a strong analgesic effect, and its analgesic strength is seven times that of aminopyrine, which is equivalent to that of peperidine. It also has significant anti-inflammatory, antipyretic, local anesthesia and nerve block effects. It is the first non-addictive analgesic new drug in China. It has no teratogenic effect and no accumulation of poisoning. It is widely used in cancer Pain and postoperative analgesia, with a total effective rate of 90.82%, is a non-addict...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D221/22
Inventor 马君义张继蔺彦斌王贵银王曼婷赵保堂
Owner NORTHWEST NORMAL UNIVERSITY
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