sTERM-1 enzyme-linked immunodetection kit and method thereof

Abstract

The invention discloses a sTERM-1 enzyme-linked immunodetection kit which comprises (1) an ELISA (enzyme-linked immunosorbent assay) plate and a detection antibody and (2) a reactant and a detection agent, wherein a capturing antibody capable of generating an antigen-antibody reaction with sTERM-1 covers and is fixed on the ELISA plate; the detection antibody can generate an antigen-antibody reaction with sTERM-1 or a combination obtained after the antigen-antibody reaction between the sTERM-1 and the capturing antibody; and the reactant and detection agent are used for detecting whether the sTERM-1 exists in a sample to be detected through enzyme-linked immunodetection. The scheme of the invention is simple in step and short in reaction time, reduces the difference of repeated experiments, is suitable for clinical detection and has great guiding significance to the prevention and diagnosis of multiple diseases, particularly acute pneumonia.

Description

technical field

[0001] The invention relates to the technical field of biomedicine, in particular to a sTERM-1 enzyme-linked immunoassay kit, a preparation method of the kit and a method for detecting sTERM-1 using the kit. technical background

[0002] TREM is a recently discovered immunoglobulin superfamily activating receptor expressed on myeloid cells. At least 5 members have been identified, among which TREM-1 is selectively expressed in neutrophils and monocytes, mediating The signal transduction pathway plays an important role in the occurrence and cascade amplification of the inflammatory response.

[0003] Although the natural ligand of TREM-1 has not yet been identified, studies have found that activation of TREM-1 using a TREM-1 competitive monoclonal antibody can cause PMN degranulation, resulting in the release of pro-inflammatory factors and proteolytic enzymes, respiratory burst and cellular Phagocytosis, and this effect can synergize with Toll-like receptor ...