Method for quantificationally characterizing cell shape features of flue-cured tobacco leaves by utilizing Photoshop software

Abstract

The invention relates to a method for quantificationally characterizing cell shape features of flue-cured tobacco leaves by utilizing Photoshop software, which is characterized by comprising the following steps of: obtaining a surface cell feature image of the preliminarily-flue-cured tobacco leaves through a scanning electron microscope, accurately selecting cells by utilizing a magnetic lasso, a matte, a paintbrush, an eraser and other tools, respectively and quantificationally obtaining the cell circumference and the cell area by using a unit pixel length*number of pixels and pixel percentage*image area method, calculating shape feature parameters through taking the circumference and the area as variables by utilizing a formula to provide a method for objectively evaluating the cell development situations of the tobacco leaves and researching the quality features of the tobacco leaves from the microscopic field. Measurement results of a plurality of cells randomly selected from different places of origin show that the method is high in accuracy and high in fitting degree of the calculated cell shape feature parameters with software-defined roundness values (R2 is equal to 0.98-0.99), and meanwhile, the cell shape features (quality features) of the tobacco leaves from the different places of origin can be better distinguished.

Description

[0001] technical field [0002] The invention relates to the field of tobacco leaf microstructure, in particular to a method for quantitatively characterizing the cell shape characteristics of cured tobacco leaves, which is mainly completed through scanning electron microscope analysis and Photoshop software image processing. [0003] Background technique [0004] Microstructure involves chemistry, biology, physics and many other fields, and refers to the structure of substances and biological cells under the microscope. In recent years, the application of scanning electron microscopy and optical microscopy to observe the micromorphological characteristics of plant organ epidermis has been widely used as a valuable new method in the study of phylogeny and quality formation. Adobe Photoshop series software has a powerful image recognition function. The pattern analysis of granite by Nishimoto et al. and the quantitative inspection of bullet casing traces by Li Nan et al. all...

AI Technical Summary

Problems solved by technology

my country's tobacco planting areas are vast, and the wide distribution and ecological diversity of tobacco leaf producing areas make the quality and style of tobacco leaves in different planting areas significantly different, and have obvious regional distribution characteristics, among which maturity, leaf structure and other appearance quality indicators and leaf Processing quality indicators such as surface density, tension, elongation, and elasticity are closely related to the morphology and structure of the cells on the surface of the tobacco leaf, and are greatly affected by the microstructure of the cells inside the tobacco leaf. At present, there is no method for quantitative characterization of the cell shape characteristics of the tobacco leaf.

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