Method for preparing sodium aescinate

A technique for sodium aescinate and time control is applied in the preparation of sugar derivatives, chemical instruments and methods, steroids, etc., and can solve the problems of long production cycle, low yield of extracting sodium aescinate, low purity and the like, Achieve the effect of improving product yield, reducing environmental pollution and reducing impurity content

Active Publication Date: 2012-09-12
WUXI KAIFU PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the above problems, the present invention provides a preparation method of sodium aescinate, which can solve the problems of low yield, low purity and long production cycle of extracting sodium aescinate that exist in tradi

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Extraction: The extraction temperature is 25°C, take 50kg of salsa seed powder and add 375L of 10% ethanol solution by volume to soak for 1 hour, extract for 2 hours, then filter to obtain the filter residue and filtrate respectively, and add the filter residue to 50L The ethanol solution with a volume percentage of 5% was extracted again for 2 hours and then filtered. The filtrates filtered twice should be clarified. The two filtrates should be combined, and the combined filtrates should be centrifuged with a plate centrifuge to collect the supernatant. The speed is 1000 rpm, and the centrifugation time is 1 hour;

[0019] (2) Chromatography: Put the washed D101 resin into the chromatography column to reach 4 / 5 column height, then add the extracted and centrifuged filtrate to the chromatography column for adsorption, the flow rate is 200 ml / min, use Acetic anhydride identification, stop adsorption when the effluent is nearly red; then wash the D101 resin with purif...

Embodiment 2

[0026] (1) Extraction: The extraction temperature is 37.5°C, take 50kg of salsa seed powder and add 150L of 10% ethanol solution by volume, soak for 1 hour, extract for 2 hours, and then filter to obtain the filter residue and filtrate respectively, and add the filter residue to 200L The ethanol solution with a volume percentage of 5% was extracted again for 2 hours and then filtered. The filtrates filtered twice should be clarified. The two filtrates should be combined, and the combined filtrates should be centrifuged with a plate centrifuge to collect the supernatant. The speed is 1300 rpm, and the centrifugation time is 1 hour;

[0027](2) Chromatography: Put the washed D201 resin into the chromatography column to reach 4 / 5 of the column height, then add the extracted and centrifuged filtrate to the chromatography column for adsorption, the flow rate is 300ml / min, and use vinegar Anhydride identification, stop adsorption when the effluent is nearly red; then wash the D201 r...

Embodiment 3

[0034] (1) Extraction: The extraction temperature is 40°C, take 50kg of salsa seed powder and add 600L of 10% ethanol solution by volume to soak for 1 hour, extract for 2 hours, then filter to obtain the filter residue and filtrate respectively, and add the filter residue to 125L The ethanol solution with a volume percentage of 5% was extracted again for 2 hours and then filtered. The filtrates filtered twice should be clarified. The two filtrates should be combined, and the combined filtrates should be centrifuged with a plate centrifuge to collect the supernatant. The speed is 1250 rpm, and the centrifugation time is 1 hour;

[0035] (2) Chromatography: put the washed NKA-9 resin into the chromatographic column to reach 4 / 5 column height, then add the extracted and centrifuged filtrate to the chromatographic column for adsorption, the flow rate is 250ml / min , identify with acetic anhydride, stop adsorption when the effluent is nearly red; then wash NKA-9 type resin with puri...

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Abstract

The invention provides a method for preparing sodium aescinate. The method can be used for solving the problem that a traditional method for preparing sodium aescinate is low in yield and purity and long in purification cycle, remaining organic solvents can be effectively decreased, the content of sodium aescinate A and sodium aescinate B is increased, accordingly, environmental pollution is decreased, and clinical application effects of the sodium aescinate are improved. The method includes the steps: firstly, performing extraction; secondly, performing purification; and finally, performing baking and drying. The method is characterized in that buckeye powder and ethanol solution are mixed and soaked according to the liquid ratio of 1:(3-12) kilograms per liter, and then are extracted, wherein the liquid ratio is preferably 1:9 kilograms per liter.

Description

technical field [0001] The invention relates to a preparation method of saponin salt, in particular to a preparation method of sodium aescinate. Background technique [0002] Sala seed is widely distributed in my country, and it is one of the few pure natural herbal medicines known to have stable curative effect and less side effects, and has a good application prospect. Sodium aescinate prepared from Sala seed has good pharmacological activities: (1) has strong anti-inflammation and anti-exudation effects; (2) reduces and eliminates the formation of edema or hematoma; (3) resists Oxygen free radicals have neuroprotective effects. With the continuous deepening of clinical research, the scope of application of sodium aescinate is also expanding, not only for cerebral edema, swelling caused by trauma or surgery, venous reflux disorders, but also for pulmonary heart disease, Hunt's syndrome, tumors, anal It also has a good curative effect on various diseases such as angioedem...

Claims

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Application Information

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IPC IPC(8): C07J63/00C07H15/256C07H1/08
Inventor 刘骞
Owner WUXI KAIFU PHARMA
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