Method for measuring contents of four tobacco-specific nitrosamines (TSNAs) in cigarette main stream smoke
A cigarette mainstream smoke and measurement method technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problem of unsatisfactory signal-to-noise ratio, unsatisfactory sensitivity, inability to fully meet the detection needs of flue-cured tobacco cigarettes, reagent consumption and heavy workload, etc. question
Active Publication Date: 2013-11-06
SHANGHAI TOBACCO GRP CO LTD +1
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Problems solved by technology
However, the GC-TEA method for detecting TSNAs in mainstream cigarette smoke was limited to the level of instruments at that time, and the required pretreatment method was complicated, and the reagent consumption and workload were large. Secondly, the signal-to-noise ratio and sensitivity of this method were not ideal, and the lower limit of quantification was generally in the 2.5ng / cig, which cannot fully meet the detection needs of flue-cured cigarettes; GC-MS is generally only suitable for cigarette smoke samples with high TSNAs content; LCMS / MS has high sensitivity and is currently a main method for detecting TSNAs at home and abroad method, but overall its detection data still have some deviation compared with GC-TEA method
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Embodiment 1
[0048] Reagents such as dichloromethane and methanol used in the examples are chromatographically pure reagents.
[0049] Configuration of standard working solution and internal standard solution:
[0050] Single standard stock solution: use dichloromethane as a solvent to prepare NNN, NNK, NAT, and NAB standard stock solutions respectively. The calculated concentration of NNN, NNK, NAT, or NAB in each single standard stock solution is 100 μg / ml, and store at 0 Under the condition of ℃~4℃, it can be stored for 6 months.
[0051] Mix internal standard stock solution (i.e. internal standard solution): use dichloromethane as solvent, prepare internal standard stock solution containing D4NNN, D4NNK, D4NAT, D4-NAB, the calculated concentration of each internal standard is 400ng / ml, Stored at 0℃~4℃, it can be stored for 6 months.
[0052] Standard working solution with internal standard: adopt the above-mentioned single standard stock solution and mixed internal standard stock sol...
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Abstract
The invention relates to a method for measuring the contents of four tobacco-specific nitrosamines (TSNAs) in cigarette main stream smoke. The method measures the contents of four tobacco-specific nitrosamines in cigarette main stream smoke by triple quadrupole gas chromatography (GC) / mass spectrometry (MS), and measures the contents of the following four tobacco-specific nitrosamines in cigarette main stream smoke by internal standard method: NNN (N-nitrosonornicotine), NNK (4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone), NAT (N-nitrosoanatabine) and NAB (N-nitrosoanabasine). The method overcomes the defects of the conventional methods for detecting the standards related to four tobacco-specific nitrosamines (TSNAs) in cigarette main stream smoke, such as complicated operation and long time duration. Besides, the method has the advantages of high sensitivity and accuracy and good repeatability; and is applied to qualitative or quantitative measurement of four tobacco-specific nitrosamines (TSNAs) in cigarette main stream smoke for technical personnel in the tobacco industry.
Description
technical field [0001] The invention relates to a method for measuring four kinds of TSNAs in mainstream smoke of cigarettes, in particular to a method for measuring the contents of four kinds of tobacco-specific nitrogen-nitrosamine TSNAs in mainstream smoke of cigarettes by adopting triple tandem gas chromatography chromatography. Background technique [0002] Tobacco-specific nitrosamines (TSNAs), one of the main cancer-inducing active ingredients in cigarette smoke, have been found to include N-nitrosonornicotine (NNN), 4-(methylnitrosamine)-1 -(3-pyridyl)-1-butanone (NNK), N-nitrosoanatabine (NAT), N-nitrosoanabatin (NAB) and other four. Among them, NNK was first discovered in cigarette smoke by Hecht et al. in 1977. Hoffmann et al. conducted a subcutaneous injection study on Syrian golden hamsters in 1981 and believed that NNK has a strong carcinogenic effect on mammals, and its carcinogenicity is the strongest among the four tobacco-specific N-nitrosamines that have ...
Claims
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IPC IPC(8): G01N30/02
Inventor 顾文博吴达陆怡峰周宛虹林华清余翀天
Owner SHANGHAI TOBACCO GRP CO LTD