Black fungus culture medium and preparation method thereof, and method for ensuring original strain inoculation survival percent of black fungus

A technology of black fungus and secondary culture medium, which is applied in the fields of botanical equipment and methods, fertilizer mixture, horticulture, etc., can solve the problems of high cost, black fungus strains are not resistant to high temperature, and it is difficult to achieve the survival rate, etc. Nutritional requirements, increased bran content, nutritionally balanced effects

Inactive Publication Date: 2012-09-26
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The cost of this medium is still high, and the original species (secondary species) with higher corn kernel content is inoculated on the third-level strains, which is easily infected by miscellaneous bacteria in the process of cultivation and sales
[0005] Since it is difficult for black fungus to germinate in low-temperature seasons, most guidance materials now advocate top-temperature inoculation, that is, to inoculate quickly while the bag temperature is still hot, so as to improve the germination rate of the fungus. In the production process of this inoculation p

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0028] Example 1. The black fungus secondary culture medium of this example is composed of the following parts by weight: 30 parts of corncobs with a diameter of 0.3 cm, 30 parts of corn kernels, 30 parts of poplar wood chips, and 10 parts of bran , 1 part of lime.

[0029] The preparation method of the black fungus secondary culture medium of this embodiment, the steps are as follows:

[0030] (1) Corn kernel treatment: After the corn has been removed, use a large pot and low heat to cook slowly, cook until there are no white hearts and basically no cracks, remove the water control, dry the surface water, and set aside;

[0031] (2) Processing of other raw materials: Stir the pre-wet corn cobs, wood chips, and bran evenly to make the moisture content 60%, and use them for later use;

[0032] (3) Mixing: Step 1) corn kernels and step 2) other raw materials are mixed evenly to obtain a black fungus secondary medium.

[0033] In this embodiment, the method for ensuring the survival rate ...

Example Embodiment

[0041] Example 2. The black fungus secondary culture medium of this example is composed of the following parts by weight: 25 parts of corn cobs with a diameter of 0.5 cm, 25 parts of corn kernels, 35 parts of tung wood chips, 12 parts of bran , 0.5 parts of lime.

[0042] The preparation method of the black fungus secondary culture medium of this embodiment, the steps are as follows:

[0043] (1) Corn kernel treatment: After the corn has been removed, use a large pot and low heat to cook slowly, cook until there are no white hearts and basically no cracks, remove the water control, dry the surface water, and set aside;

[0044] (2) Processing of other raw materials: Stir the pre-wet corn cobs, wood chips, and bran evenly to make the moisture content 62%, and set aside;

[0045] (3) Mixing: Step 1) corn kernels and step 2) other raw materials are mixed evenly to obtain a black fungus secondary medium.

[0046] In this embodiment, the method for ensuring the survival rate of black fungus...

Example Embodiment

[0054] Example 3. The black fungus secondary culture medium of this example is composed of the following parts by weight: 35 parts of corncobs with a diameter of 0.3 cm, 20 parts of corn kernels, 25 parts of poplar wood chips, 15 parts of bran , 1.5 parts of lime.

[0055] The preparation method of the black fungus secondary culture medium of this embodiment, the steps are as follows:

[0056] (1) Corn kernel treatment: After the corn has been removed, use a large pot and low heat to cook slowly, cook until there are no white hearts and basically no cracks, remove the water control, dry the surface water, and set aside;

[0057] (2) Processing of other raw materials: Stir the pre-wet corncobs, wood chips, and bran evenly to make the moisture content 65%, and use them for later use;

[0058] (3) Mixing: Step 1) corn kernels and step 2) other raw materials are mixed evenly to obtain a black fungus secondary medium.

[0059] In this embodiment, the method for ensuring the survival rate of...

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PUM

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Abstract

The invention discloses a black fungus secondary culture medium which is composed of corn cob particles, corn grains, wood dust, bran and lime. The black fungus secondary culture medium has the advantages of reasonable formula and balanced nutrition, and can well satisfy the nutritional requirements for black fungus. The corn cob is added, so that the cost of the culture medium disclosed by the invention is lower than that of other wood dust/corn grain culture media; and the bran content is increased, so that the black fungus hyphae can grow more vigorously. The invention also discloses a method for ensuring original strain survival percent of black fungus, which optimizes inoculation and culture techniques. After the inoculation, the strain survival percent is up to 100%, the inoculation survival percent of the original strain is ensured, the hypha growth rate is high, and the hyphae are white, thick, strong and vigorous and have strong growth potential.

Description

technical field [0001] The invention relates to a black fungus cultivation technology, a secondary black fungus culture medium, and a method for ensuring the survival rate of black fungus original seed inoculation. Background technique [0002] Black fungus is rich in protein and multivitamins. It has the effects of cleaning the stomach, reducing blood clots, and relieving coronary atherosclerosis. It has the reputation of "the meat of the vegetarian". With the continuous improvement of people's living standards, the demand for black fungus is also increasing. At present, a small part of black fungus on the market is collected wild, and most of them are artificially cultivated. There are two methods of artificial cultivation: segment wood cultivation and plastic bag material cultivation. Segment wood cultivation of black fungus seriously destroys forest data, therefore, the focus of current research is bagged cultivation. [0003] Generally, the production process of blac...

Claims

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Application Information

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IPC IPC(8): C05G3/00A01G1/04
Inventor 侯军林晓民李冬琳孙亚莉张虹
Owner HENAN UNIV OF SCI & TECH
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