Method for extracting starch and cucurbitacins from siraitia grosvenorii roots

A technique of Luo Han Guo and cucurbitacin, which is applied in the fields of simultaneously extracting amylopectin and cucurbitacin, and extracting starch and cucurbitacin, can solve the problems such as the root of Luo Han Guo not being really well utilized, and achieves increasing the scope of use, improving the utilization rate, The effect of saving starch resources

Inactive Publication Date: 2012-09-26
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0027] In summary, the current utilization of Luo Han Guo mainly uses the fruit to extract mogroside V, and mogroside V is used as a sweetener, while the root of Luo Han Guo mainly stays in the analysis of chemical components, and the root of Luo Han Guo has not really been well obtained. use of

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Wash the fresh Luo Han Guo tubers with water, take 200 kg of tubers and add 200 L of water to grind them, leave them at room temperature for 1 hour, add 300 L of petroleum ether for extraction, take the upper layer solution to obtain a solution containing petroleum ether, and reduce the solution containing petroleum ether at 70 ° C. Concentrate under pressure to 80L, put the concentrated solution on NKA-9 macroporous adsorption resin column, use ethanol concentration of 20-60%, gradient elution, collect 200L of eluent I with 60% ethanol; concentrate eluent I to 25L, Add 25L of acetone solution, crystallize and recrystallize overnight at room temperature to obtain 3.56kg of pure cucurbitacin; stir and refine the lower layer material without petroleum ether, and then pass through a 10-mesh sieve to obtain starch milk, and rinse the Luo Han Guo pulp with 200L of water. Centrifuge at a low speed of 3000-4000 rpm to obtain a precipitate, then dry and dehydrate at a low speed ...

Embodiment 2

[0051] Wash the fresh Luo Han Guo tubers with water, take 400 kg of tubers and add 500 L of water to grind them, leave them at room temperature for 1 hour, add 600 L of petroleum ether for extraction, take the upper layer solution to obtain a solution containing petroleum ether, and reduce the solution containing petroleum ether at 70 ° C. Concentrate under pressure to 100L, put the concentrated solution on NKA-9 macroporous adsorption resin column, use ethanol concentration of 20-60%, gradient elution, collect 250L of eluent I with 60% ethanol; concentrate eluent I to 25L, Add 25L of acetone solution, crystallize and recrystallize overnight at room temperature, and obtain 7.34kg of pure cucurbitacin; stir and refine the lower layer material without petroleum ether, and then pass through a 10-mesh sieve to obtain starch milk, and rinse the monk fruit pulp with 600L of water. Centrifuge at a low speed of 3000-4000 rpm to obtain a precipitate, then dry and dehydrate at a low spee...

Embodiment 3

[0053] Wash fresh Luo Han Guo tubers with water, take 500 kg of tubers and add 900 L of water to grind them, leave them at room temperature for 1 hour, add 1000 L of petroleum ether for extraction, take the upper solution to obtain a solution containing petroleum ether, and reduce the solution containing petroleum ether at 70 ° C. Concentrate under pressure to 120L, put the concentrated solution on NKA-9 macroporous adsorption resin column, use ethanol concentration of 20-60%, gradient elution, collect 250L of eluent I with 60% ethanol; concentrate eluent I to 30L, Add 30L of acetone solution, crystallize and recrystallize overnight at room temperature to obtain pure cucurbitacin; stir and refine the lower layer material without petroleum ether, and then pass through a 10-mesh sieve to obtain starch milk, rinse Luo Han Guo pulp with 900L of water, and pass through 3000 - 4000 rev / min low-speed centrifugation to obtain a precipitate, and carry out low-speed drying and dehydratio...

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Abstract

A pure cucurbitacin product is obtained through conducting grinding, petroleum ether leaching and filtering on siraitia grosvenorii roots, adsorbing by resin after reduced pressure concentration on filtrate, eluting by different concentrations of eluents, decoloring the eluents, crystallizing and recrystallizing, and starch is obtained by jordaning filter residue, filtering to obtain starch milk, precipitating the starch milk through centrifugal separation, and drying or dehydrating 95% of ethanol. The cucurbitacin substances and the starch substances can be obtained at the same time by the method, so that the utilization rate of the siraitia grosvenorii roots is improved greatly, the starch resource is saved, the operation is simple, the residual of cucurbitacins in the starch in siraitia grosvenorii roots is reduced greatly, and the use scope of starch is expanded.

Description

technical field [0001] The invention belongs to the field of natural product processing, and relates to a method for extracting starch and cucurbitacin, and more specifically relates to a method for simultaneously extracting amylopectin and cucurbitacin from the roots of Luo Han Guo. Background technique [0002] The traditional Chinese medicine Luo Han Guo is the ripe fruit of Cucurbitaceae Luo Han Guo. It is a precious specialty and traditional export medicinal material in my country. It has been reported in the literature that it contains three mushroom saponins and flavonoid glycosides. Its sweet ingredients are ideal natural sweeteners. Luo Han Guo has a large root, bitter taste, and slightly cold nature. It has the effects of clearing away heat and dampness, dredging collaterals and relieving pain. It is a commonly used medicinal material in Guangxi. Pharmacological experiments show that the extract of Luo Han Guo root has anti-inflammatory, analgesic, antispasmodic, e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B30/00C07J9/00
Inventor 饶力群
Owner HUNAN AGRICULTURAL UNIV
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