Method for extracting starch and cucurbitacins from siraitia grosvenorii roots
A technique of Luo Han Guo and cucurbitacin, which is applied in the fields of simultaneously extracting amylopectin and cucurbitacin, and extracting starch and cucurbitacin, can solve the problems such as the root of Luo Han Guo not being really well utilized, and achieves increasing the scope of use, improving the utilization rate, The effect of saving starch resources
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Embodiment 1
[0049] Wash the fresh Luo Han Guo tubers with water, take 200 kg of tubers and add 200 L of water to grind them, leave them at room temperature for 1 hour, add 300 L of petroleum ether for extraction, take the upper layer solution to obtain a solution containing petroleum ether, and reduce the solution containing petroleum ether at 70 ° C. Concentrate under pressure to 80L, put the concentrated solution on NKA-9 macroporous adsorption resin column, use ethanol concentration of 20-60%, gradient elution, collect 200L of eluent I with 60% ethanol; concentrate eluent I to 25L, Add 25L of acetone solution, crystallize and recrystallize overnight at room temperature to obtain 3.56kg of pure cucurbitacin; stir and refine the lower layer material without petroleum ether, and then pass through a 10-mesh sieve to obtain starch milk, and rinse the Luo Han Guo pulp with 200L of water. Centrifuge at a low speed of 3000-4000 rpm to obtain a precipitate, then dry and dehydrate at a low speed ...
Embodiment 2
[0051] Wash the fresh Luo Han Guo tubers with water, take 400 kg of tubers and add 500 L of water to grind them, leave them at room temperature for 1 hour, add 600 L of petroleum ether for extraction, take the upper layer solution to obtain a solution containing petroleum ether, and reduce the solution containing petroleum ether at 70 ° C. Concentrate under pressure to 100L, put the concentrated solution on NKA-9 macroporous adsorption resin column, use ethanol concentration of 20-60%, gradient elution, collect 250L of eluent I with 60% ethanol; concentrate eluent I to 25L, Add 25L of acetone solution, crystallize and recrystallize overnight at room temperature, and obtain 7.34kg of pure cucurbitacin; stir and refine the lower layer material without petroleum ether, and then pass through a 10-mesh sieve to obtain starch milk, and rinse the monk fruit pulp with 600L of water. Centrifuge at a low speed of 3000-4000 rpm to obtain a precipitate, then dry and dehydrate at a low spee...
Embodiment 3
[0053] Wash fresh Luo Han Guo tubers with water, take 500 kg of tubers and add 900 L of water to grind them, leave them at room temperature for 1 hour, add 1000 L of petroleum ether for extraction, take the upper solution to obtain a solution containing petroleum ether, and reduce the solution containing petroleum ether at 70 ° C. Concentrate under pressure to 120L, put the concentrated solution on NKA-9 macroporous adsorption resin column, use ethanol concentration of 20-60%, gradient elution, collect 250L of eluent I with 60% ethanol; concentrate eluent I to 30L, Add 30L of acetone solution, crystallize and recrystallize overnight at room temperature to obtain pure cucurbitacin; stir and refine the lower layer material without petroleum ether, and then pass through a 10-mesh sieve to obtain starch milk, rinse Luo Han Guo pulp with 900L of water, and pass through 3000 - 4000 rev / min low-speed centrifugation to obtain a precipitate, and carry out low-speed drying and dehydratio...
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