Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for separation and purification of homoarbutin and hyperoside from P. calliantha H Andr

A high arbutin, separation and purification technology, applied in the field of separation and purification of high arbutin and hypericin, can solve the problems of increased cost, long time consumption, large loss of target compounds, etc. Repeatability and high product yield

Inactive Publication Date: 2012-10-17
NORTHEAST FORESTRY UNIVERSITY +1
View PDF5 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] To sum up, in the process of separating and purifying high arbutin and hyperoside by using plant extracts, the main influencing factors are that the separation process generally takes a long time and the loss of target compounds is large, which increases the cost

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for separation and purification of homoarbutin and hyperoside from P. calliantha H Andr
  • Method for separation and purification of homoarbutin and hyperoside from P. calliantha H Andr

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Weigh 500g of crushed wintergreen whole plant, add 10 times the volume of 85% ethanol, and carry out ultrasonic-assisted extraction. The extraction time is 40min, the temperature is 40-50°C, and the power is 36KHz. The above process was performed twice, and the three extracts were combined and concentrated under reduced pressure to a density of 1.15. Prepare 89.3 g of the solid matter obtained by extraction and concentration with water to prepare a suspension drug solution with a feed concentration of 15 mg / ml. The sample volume is 0.6 times the column volume, and the AB-8 macroporous adsorption is carried out under the condition of an elution flow rate of 7 ml / min. Resin, after adsorption, wash impurities with 2 times column volume of water, and then use 6 times column volume in turn, and carry out gradient elution and desorption with solvent systems with ethanol concentrations of 10%, 30%, 50% and 70% respectively, and collect each gradient Combine the eluents with et...

Embodiment 2

[0027]Weigh 300g of crushed wintergreen whole plant, add 15 times the volume of 75% ethanol, and carry out ultrasonic-assisted extraction. The extraction time is 50min, the temperature is 40-50°C, and the power is 32KHz. The above process was performed twice, and the three extracts were combined and concentrated under reduced pressure to a density of 1.20. Prepare 57.1 g of the solid matter obtained by extraction and concentration with water to prepare a suspension drug solution with a feed concentration of 20 mg / ml. The sample volume is 0.8 times the column volume, and the NKA-9 macroporous adsorption is carried out under the condition of an elution flow rate of 8 ml / min. Resin, after adsorption, wash impurities with 2 times column volume of water, then use 5 times column volume, solvent system with ethanol concentration of 10%, 30%, 50% and 70% respectively for gradient elution and desorption, collect each gradient Combine the eluents with ethanol concentrations of 30-50% an...

Embodiment 3

[0031] Weigh 1000g of crushed wintergreen whole plant, add 12 times the volume of 70% ethanol, and carry out hot reflux extraction, extraction time is 50min, temperature is 75°C, after extraction, the extract is filtered out, and the above process is repeated twice, The three extracts were combined and concentrated under reduced pressure to a density of 1.20. The solid matter 182.4g obtained by extracting and concentrating is formulated with water to prepare a suspension liquid with a feed concentration of 25mg / ml, and the loading amount is 1 times the column volume, and the D101 type macroporous adsorption resin is passed under the condition of an elution flow rate of 10ml / min. After adsorption, wash the impurities with 2 times the column volume of water, and then use 6 times the column volume and solvent systems with ethanol concentrations of 10%, 30%, 50% and 70% for gradient elution and desorption, and collect the washes of each gradient respectively. Remove the liquid, co...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for extraction, separation and purification of active ingredients from Chinese herbal medicines, specifically to a method for extraction, separation and purification of the active ingredients homoarbutin and hyperoside from P. calliantha H Andr. Aiming to provide a simple, safe, economical and effective method for separation and purification of homoarbutin and hyperoside from P. calliantha H Andr, the invention adopts the following technical solution of: crushing a dry P. calliantha H Andr whole plant, conducting extraction with ethanol to obtain an extract, then passing the extract through macroporous adsorption resin, Sephadex LH-20 gel and an ODS-C18 reverse phase medium pressure silica gel column for chromatographic separation, and combining low temperature crystallization and recrystallization technologies to obtain refined homoarbutin and hyperoside with purity of over 95%. The method provided in the invention has the advantages of rich and easily available raw materials, simple and practicable process, high yield, high purity, safety and environment friendly operation, thus being suitable for industrial application.

Description

technical field [0001] The invention relates to a method for separating and purifying functional components from plants, in particular to separating and purifying homoarbutin and hyperin from whole wintergreen plants. Background technique [0002] Wintergreen (P. calliantha H Andr) is a perennial evergreen herb of the Wintergreen family. Also known as Luxiancao, Lushoucao, Poxuedan, etc., it is mainly distributed in the northeast, southwest, and southeast of my country. It is a commonly used Chinese herbal medicine among the people. Wintergreen has the functions of invigorating deficiency, nourishing kidney, promoting blood circulation, nourishing qi and blood, hemostasis, strengthening muscles and bones, diuresis, and antibacterial; it is used to treat weak tuberculosis, cough, night sweats, nocturnal emission, weak waist and knee leucorrhea, tonify kidney and strengthen yang, regulate menstruation and activate blood circulation Treat traumatic bleeding, bites by insects, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H15/203C07H1/08
Inventor 付玉杰祖元刚姚晓慧
Owner NORTHEAST FORESTRY UNIVERSITY