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Extraction method of salvia miltiorrhiza polysaccharide from tanshinol residues

A technology of salvia alcohol and residue, applied in the field of extracting salvia miltiorrhiza polysaccharide from salvia alcohol extraction residue, can solve the problem of low extraction rate of salvia miltiorrhiza polysaccharide, and achieve the effects of saving protein removal process, saving resources and improving efficiency.

Inactive Publication Date: 2014-06-18
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] For the extraction of Danshen polysaccharides, patent No. CN101225422 patent A proposes a method of ultrasonically extracting Danshen polysaccharides after cellulase and papain enzymatic hydrolysis, but the extraction rate of Danshen polysaccharides is only 5%-11%.

Method used

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  • Extraction method of salvia miltiorrhiza polysaccharide from tanshinol residues
  • Extraction method of salvia miltiorrhiza polysaccharide from tanshinol residues
  • Extraction method of salvia miltiorrhiza polysaccharide from tanshinol residues

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Embodiment 1

[0022] 1. Materials and reagents

[0023] Alcohol extraction residue of Danshen; cellulase, pectinase, papain, absolute ethanol, concentrated sulfuric acid (excellent grade), glucose, phenol, 3-5-dinitrosalicylic acid, potassium sodium tartrate, unless otherwise specified Others are analytically pure.

[0024] 2. Experimental equipment

[0025] UV-visible spectrophotometer (ShimazuUV-1750), PHS-320 intelligent acidity meter, HH.S11-Ni6 electric heating constant temperature water bath, KQ-300GDV constant temperature numerical control ultrasonic cleaner, sartorius series electronic balance, GL-2050M high-speed refrigerated centrifugal Machine, DZ-2BC vacuum drying oven, RE-52AA / RE-52A rotary evaporator, etc.

[0026] 3 Experimental methods

[0027] 3.1 Technical route of polysaccharide extraction

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Abstract

The invention provides an extraction method of salvia miltiorrhiza polysaccharide from tanshinol residues. The salvia miltiorrhiza polysaccharide is prepared by performing extracting, suction filtering, concentrating, dialyzing, alcohol precipitating, suction filtering, vacuum drying, content detecting and other process flows to salvia miltiorrhiza residues which are subjected to alcohol extraction in an ultrasonic-assisted complex enzymes method, wherein the polysaccharide yield is 11.46 percent, and the polysaccharide content is over 80 percent. Residues after industrial production of salvia miltiorrhiza alcohol extract are used as materials in the method, sustainable utilization of resources can be realized, the production cost of salvia miltiorrhiza and the treatment cost of industrial waste can be saved, the extraction steps of the salvia miltiorrhiza polysaccharide can be reduced, resources are saved, and efficiency can be improved. According to the extraction method, the salvia miltiorrhiza polysaccharide is extracted by adopting the ultrasonic-assisted complex enzymes method, the adopted papain has good protein removal effect, and extra protein removal process can be saved. The product prepared by the method has high purity, wherein the polysaccharide content is over 80 percent.

Description

technical field [0001] The invention belongs to the field of comprehensive utilization of traditional Chinese medicine resources, and relates to secondary resource development of salvia miltiorrhiza waste, in particular to a method for extracting salvia miltiorrhiza polysaccharide from alcohol extraction residue of salvia miltiorrhiza. Background technique [0002] Salviamiltiorrhiza Bunge is a perennial herb of the genus Salvia Linn. in the family Lamiaceae. It is slightly cold in nature, bitter in taste, non-toxic, and has the effects of promoting blood circulation, promoting menstrual flow, dispelling blood stasis and relieving pain, clearing the heart and calming the nerves. Pharmacological studies have shown that Salvia miltiorrhiza not only has a very significant effect on the cardiovascular system, but also has anti-oxidation, anti-coagulation, anti-thrombotic, anti-tumor, anti-inflammatory, anti-bacterial, liver-protecting and other immunoregulatory effects. The mai...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00
Inventor 张利姜媛媛王龙喻麟王萌王涛刘超
Owner SICHUAN AGRI UNIV