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Preparation method of L-alpha glyceryl phosphorylethanolamine

A technology of glycerol phosphatidylethanolamine and phospholipids, applied in the field of preparation of glycerol phosphatidylethanolamine, which can solve the problems of environmental pollution, low separation efficiency, and low optical purity

Inactive Publication Date: 2012-10-17
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The invention provides a preparation method of L-α-GPE, which solves the problems of low conversion rate, low optical purity and environmental pollution in the process of producing L-α-GPE, and also solves the separation and purification problems Problems such as low efficiency, low recovery rate and complicated operation

Method used

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  • Preparation method of L-alpha glyceryl phosphorylethanolamine
  • Preparation method of L-alpha glyceryl phosphorylethanolamine
  • Preparation method of L-alpha glyceryl phosphorylethanolamine

Examples

Experimental program
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Embodiment 1

[0026] The raw material used is food soybean powder phospholipid (wherein the PC content is about 24%).

[0027] Lipase hydrolysis reaction: add 50g powdered phospholipids, 1000mL deionized water into a three-hole flask, heat in a water bath at 35°C, mechanically stir at 300r / min, homogenize for 30min, add CaCl 2 2 g, homogenized, added 5 mL of Rhizopus sinica lipase, reacted for 360 min, centrifuged the enzyme reaction liquid, and filtered it with suction to obtain a pure enzymatic hydrolysis reaction liquid.

Embodiment 2

[0029] The enzymolysis reaction solution used is the product obtained in Example 1.

[0030] Resin column chromatography to remove ions in the enzyme reaction solution: the enzymolysis reaction solution passes through a 001X7 strong cation exchange resin column (exchange capacity 1800mmol / L) to remove Ca 2+ , through the D311 macroporous acrylic series weak base anion exchange resin column (2200mmol / L) to remove Cl - , 60 ° C, vacuum concentration, ion exchange resin regeneration.

Embodiment 3

[0032] The enzymolysis reaction solution used is the product obtained after removing ions in Example 2.

[0033] Purification of L-α-GPE by silica gel column chromatography: Weigh 2.97g of L-α-GPE, dissolve in 50mL ethanol, weigh 148.5g of silica gel, dissolve in ethanol, stir and homogenize, overnight, pack into column, compact, load sample, Elute with a certain proportion of ethanol aqueous solution (1:9, v:v), flow rate 2mL / min, collect every 30mL fraction, detect each fraction by HPLC-ELSD, combine similar fractions, and separate L-α- GPE, L-α-GPC and other by-products are separated, the silica gel is recovered, and the silica gel is regenerated in an oven at 120°C for 180 minutes;

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Abstract

The invention discloses a preparation method of L-alpha glyceryl phosphorylethanolamine (L-alpha-GPE), belonging to the technical field of development and application of lipid. The method comprises the following steps: using soya phospholipides powder as a raw material, in water phase, using phosphatidylethanolamine (PE) to produce L-alpha-GPE; respectively removing cations and anions by using the column chromatography of cation and anion exchange resin, purifying the column chromatography of silica gel, decoloring the active carbon, and conducting vacuum concentration to obtain the colorless and transparent L-alpha-GPE. By conducting HPLC-ELSD analysis, the chemical purity is 99.6%, the recovery rate is 66.5%, and by analysis through a polarimeter, the optical purity ee is 99%, and [alpha]<20>D=-3.26 degrees (H2Ocontent is 15.7%).

Description

technical field [0001] A preparation method of glycerol phosphatidylethanolamine (L-alpha glycerylphosphorylethanolamine, L-α-GPE) relates to the fields of food chemistry, fine chemistry, organic chemistry and medicinal chemistry. Background technique [0002] Glycerol phosphatidylethanolamine (L-alpha glycerylphosphorylethanolamine, L-α-GPE) is a naturally occurring water-soluble phospholipid metabolite in the body, and its atomic structure is shown figure 1 shown. It mainly exists in the liver of pigs and rabbits, and the brain of cattle, and is the precursor for the biosynthesis of phospholipids such as cephalin, lecithin and phosphatidylserine in organisms. L-α-GPE has important medical application value. It can treat various neurological and psychological function defects of the human body, such as slow mental activity, memory decline with age, emotional instability and emotional fragility, etc. aspects of the disease. [0003] At present, the methods for preparing L...

Claims

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Application Information

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IPC IPC(8): C12P13/00C07F9/10
Inventor 刘元法张康逸周丽孟宗王兴国宋志华
Owner JIANGNAN UNIV
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