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Electrochemical immunosensor, preparation method and use of electrochemical immunosensor

An immunosensor, electrochemical technology, applied in the direction of material electrochemical variables, material inspection products, biological testing, etc., to achieve the effects of fast detection speed, wide detection range, and simple operation

Active Publication Date: 2013-07-17
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods have some limitations in sensitivity, selectivity, stability and operability

Method used

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  • Electrochemical immunosensor, preparation method and use of electrochemical immunosensor

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Experimental program
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Effect test

Embodiment 1

[0047] (1) Synthesis of sulfonated graphene

[0048] 37.5 mg of graphite oxide was dispersed in 37.5 g of water. Then a brown graphene oxide suspension was obtained after ultrasonication for 1 h.

[0049]Pre-reduction: first use 5wt% sodium carbonate solution to adjust the pH of the above-mentioned graphene oxide suspension to 9, mix 300mg NaBH 4 Dissolve in 7.5g water, and then add NaBH 4 The solution was added to the graphene oxide suspension. The mixture was stirred continuously at 80 °C for 1 h. The reduction process was carried out under nitrogen atmosphere. The suspension turned from coffee color to black. Aggregation was observed after the reduction reaction, and after centrifugation and washing with water for 2 to 3 times, the partially reduced graphene oxide was dispersed in 37.5 g of water by ultrasound to obtain a suspension;

[0050] Sulfonation: 23mg p-aminobenzenesulfonic acid and 9mg sodium nitrite react in 5g water and 0.25g 1mol / L HCl, forming diazonium ...

Embodiment 2

[0070] (1) Synthesis of sulfonated graphene

[0071] 37.5 mg of graphite oxide was dispersed in 37.5 g of water. Then a brown graphene oxide suspension was obtained after ultrasonication for 1 h.

[0072] Pre-reduction: first adjust the pH of the above-mentioned graphene oxide suspension to 10 with 5wt% sodium carbonate, mix 300mg NaBH 4 Dissolve in 7.5g water, and then add NaBH 4 The solution was added to the above graphene oxide suspension. The mixture was stirred continuously at 80 °C for 1 h. The reduction process was carried out under nitrogen atmosphere. The suspension turned from coffee color to black. Aggregation was observed after the reduction reaction, and after centrifugation and washing with water for 2 to 3 times, the partially reduced graphene oxide was dispersed in 37.5 g of water by ultrasound to obtain a suspension;

[0073] Sulfonation: 23mg of p-aminobenzenesulfonic acid and 9mg of sodium nitrite react in 5g of water and 0.25g of 1mol / L HCl to form di...

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Abstract

The invention relates to an electrochemical immunosensor, and a preparation method and use of the electrochemical immunosensor. The preparation method comprises the following steps: firstly, introducing a metal nanoparticles-mesoporous material compound (Pd hybrid SBA-15) into preparation of the electrochemical immunosensor of human chorionic gonadotropin (hCG); dispensing the compound of sulfated grapheme and thionine on the surface of a glassy carbon electrode to form a composite membrane with high surface energy and good redox-activity; taking thionine as an electronic mediator, taking glutaraldehyde as a cross-linking agent, covalently bonding Pd hybrid SBA-15 mesoporous material with amino, and preparing the hCG electrochemical immunosensor based on Pd hybrid SBA-15, drawing a work curve under the optimum condition and achieving measurement of hCG in a serum sample. The electrochemical immunosensor disclosed by the invention has the advantages of high sensitivity, good reproducibility and simple and fast operation in detection of hCG, and provides a new approach for detection of other tumor markers.

Description

technical field [0001] The present invention relates to an electrochemical immunosensor, its preparation method and its application, more specifically, the present invention relates to an electrochemical immunosensor based on Pd hybrid SBA-15 (mesoporous silica molecular sieve), its preparation The method, and the application of the electrochemical immunosensor prepared by the method in the determination of human chorionic gonadotropin. Background technique [0002] Human chorionic gonadotropin (hCG) is a sialoglycoprotein secreted mainly by trophoblast cells of placental tissue. Under normal circumstances, concentrations in healthy, non-pregnant individuals are low and may not be detectable. Ectopic pregnancy, many malignant non-trophoblast tumors and non-gonadal tumors can produce ectopic hCG, so the detection of hCG has become a common method to predict early pregnancy and miscarriage, and to help diagnose ectopic pregnancy and related diseases. Currently, hCG detection...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26G01N33/76
Inventor 吴丹魏琴张勇马洪敏杜斌蔡燕燕桑欣欣
Owner UNIV OF JINAN