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Scorpion venom protein polypeptide colon-targeted preparation and preparation method thereof

A technology of scorpion venom protein and colon targeting, which is applied in the direction of peptide/protein components, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., to achieve simple production process, improve drug compliance, and drug administration safety convenient effect

Inactive Publication Date: 2014-02-05
FUJIAN HEALTH COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, no colon-targeting preparation containing scorpion venom has been seen in the domestic market, and there are no research reports on this drug preparation. Therefore, the development of this type of drug not only has important clinical significance, but also has broad market prospects.

Method used

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  • Scorpion venom protein polypeptide colon-targeted preparation and preparation method thereof
  • Scorpion venom protein polypeptide colon-targeted preparation and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0044] Weigh 10g of scorpion venom, and accurately weigh low-ester pectin according to the mass ratio of scorpion venom to low-ester pectin at 1:20. Sprinkle low-ester pectin into purified water in stages, dissolve it into a solution with a concentration of 8% by mass and volume, add scorpion venom powder, stir to dissolve, and remove foam by ultrasonic vibration. Use a No. 6 injection needle to drop the mixed solution into the slowly stirred volume of 50 mL, and the mass volume percentage concentration of 10% CaCl 2 In the solution, solidify for 30 minutes, filter, and dry at 60°C, then soak the pellets in a 1.0% PEI (polyethyleneimine) solution by mass volume percentage, cross-link for 6 hours, filter, wash with purified water and dry at 60°C dry. The colon targeting preparation is obtained.

Embodiment 2

[0046] Weigh 5g of scorpion venom, and accurately weigh low-ester pectin according to the mass ratio of scorpion venom to low-ester pectin of 1:10. Sprinkle low-ester pectin into purified water in stages, dissolve it into a solution with a concentration of 6% by mass and volume, add scorpion venom powder, stir to dissolve, and remove foam by ultrasonic vibration. Use a 6-gauge injection needle to drop the mixed solution into the slowly stirred volume of 50 mL and the mass volume percentage concentration of 8% CaCl 2 In the solution, solidify for 30 minutes, filter, and dry at 60°C, then soak the pellets in a 0.8% PEI solution by mass volume percentage, cross-link for 6 hours, filter, wash with purified water and dry at 60°C. The colon targeting preparation is obtained.

Embodiment 3

[0048] Weigh 5g of scorpion venom and 10g of flufenamic acid (passed through an 80-mesh sieve), and accurately weigh low-ester pectin according to the mass ratio of medicine to low-ester pectin of 1:15. Sprinkle low-ester pectin into purified water in batches, dissolve it into a solution with a concentration of 8% by mass and volume, add scorpion venom and flufenamic acid, stir and suspend it evenly, and remove foam by ultrasonic vibration. Use a No. 6 injection needle to drop the mixed solution into the slowly stirred volume of 50 mL, and the mass volume percentage concentration of 10% CaCl 2 In the solution, solidify for 30 minutes, filter, and dry at 60°C, then soak the pellets in a 1.0% PEI solution with mass volume percentage, cross-link for 6 hours, filter, wash with purified water, and dry at 60°C. The colon targeting preparation is obtained.

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Abstract

The invention discloses a scorpion venom protein polypeptide colon-targeted preparation and a preparation method thereof. Low-ester pectin is used as a main carrier and dripped into solution containing calcium ions, pellets are formed after reaction and solidification of the low-ester pectin, the pellets are soaked in polymine solution with a certain concentration and further cross-linked after being dried to form outer layers with higher hydrophobicity, and the scorpion venom protein polypeptide colon-targeted preparation is obtained after drying. After the preparation is taken orally, medicines are concentrated to release at the position of a colon, the concentration of the medicines at the position of the colon can be remarkably improved, the medicines directly contact with a focus of the colon and can be effectively prevented from being damaged by gastric acid or enzyme and the like in a stomach and a small intestine, and therapeutic effects of the medicines are made full use of.

Description

technical field [0001] The invention belongs to the field of pharmaceutical preparations, and relates to a colon-targeting preparation of a scorpion venom polypeptide and a preparation method thereof. More specifically, the present invention relates to a pellet that can target the colon to release scorpion venom and stay in the colon for a long time and its preparation method. Background technique [0002] Scorpion venom is mainly composed of protein and non-protein. The main active ingredient of scorpion venom is protein, and the active protein is divided into toxic protein (scorpion toxin) and enzyme according to different functions. Scorpion toxin (Scor-pionvenom) is a kind of toxic protein composed of 20-80 amino acids containing C, H, O, N, S and other elements. The toxin has high specificity, high sulfur content, and a molecular weight of Between 6000 and 9000, but there are also about 3000 or more than 10000. The molecular structure of scorpion toxin contains 3 to 4...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/10A61K38/17A61K47/48A61K47/36A61K47/38A61P1/00A61P1/04A61P7/02A61P25/04A61P29/02A61P35/00A61K31/196A61K31/606A61K31/665
Inventor 黄仁杰林国威叶榕肖健赖成敏
Owner FUJIAN HEALTH COLLEGE