Purification method for two active monomer compounds in saxifrage and application of product of the same

A technology of active monomers and saxifrage, applied in chemical instruments and methods, organic active ingredients, medical preparations containing active ingredients, etc., can solve the problems of undiscovered active ingredients

Inactive Publication Date: 2012-11-07
GUIZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are few pharmacological studies on the anti-prostate cancer of saxifrage extract, and no active ingredients have been found

Method used

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  • Purification method for two active monomer compounds in saxifrage and application of product of the same
  • Purification method for two active monomer compounds in saxifrage and application of product of the same
  • Purification method for two active monomer compounds in saxifrage and application of product of the same

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Embodiment 1 of the present invention: the preparation of saxifrage extract:

[0064] Take the dried saxifrage whole herb, crush it into a coarse powder, add 8 times the volume of 80% ethanol to reflux extraction twice, each time for 2 hours, filter, combine the filtrate, recover ethanol under reduced pressure, and concentrate the remaining liquid to a relative density of 1.25 thick paste, that is, saxifrage extract Ⅰ.

Embodiment 2

[0065] Embodiment 2 of the present invention: preparation of saxifrage extract:

[0066] Take the dried saxifrage whole herb, crush it into a coarse powder, add 8 times the volume of 80% ethanol to reflux extraction twice, each time for 2 hours, filter, combine the filtrate, recover ethanol under reduced pressure, and concentrate the remaining liquid to a relative density of The thick paste of 1.25 was extracted twice with 4 volumes of petroleum ether and ethyl acetate respectively, and the ethyl acetate extracts were combined and evaporated to dryness under reduced pressure to obtain the saxifrage extract II.

Embodiment 3

[0067] Embodiment 3 of the present invention: Extraction and purification of two active monomer compounds in saxifrage:

[0068] (1) Take the dried saxifrage whole herb, crush it into a coarse powder, add 8 times the volume of 80% ethanol to reflux and extract twice, each time for 2 hours, filter, combine the filtrates, recover ethanol under reduced pressure, and concentrate the remaining liquid to Relative density is the thick ointment of 1.25, obtains Saxifraga ethanol extract;

[0069] (2) The ethanol extract of saxifrage was extracted twice with 4 times the volume of petroleum ether and ethyl acetate in sequence, and the ethyl acetate extract was combined, and evaporated to dryness under reduced pressure to obtain the ethyl acetate extract;

[0070](3) The ethyl acetate extract was segmented using 200-300 mesh column chromatography silica gel, and gradient eluted with petroleum ether: ethyl acetate = 30:1-1:1 and chloroform: methanol = 30:1-0:1; The highly polar part of e...

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Abstract

The invention discloses a purification method for two active monomer compounds in saxifrage and application of product of the same. The method is as below: extracting saxifrage with ethanol; extracting the extract successively with petroleum ether and ethyl acetate; and separating the ethyl acetate extract by silica gel column chromatography, medium pressure column chromatography and gel column chromatography, so as to separate out the two monomer compounds (quercetin 3-O-beta-L-rhamnoside, quercetin 5-O-beta-D glucoside) from saxifrage. Biological activity determination results indicate that the saxifrage extract and the two monomer compounds have good inhibition effect on growth and proliferation of prostate cancer cells, can be used in preparation of medicine for treating prostate cancer, and have good application prospects in drugs for treating prostate cancer.

Description

technical field [0001] The invention relates to a method for extracting and purifying two active monomer compounds in saxifrage and the application of the product, belonging to the technical field of traditional Chinese medicine. Background technique [0002] Prostate cancer is a malignant tumor that occurs in male prostate tissue and is the result of abnormal and disordered growth of prostate acinar cells. Prostate cancer incidence varies markedly by geographic and ethnic origin. In developed countries and regions such as Europe and the United States, it is the most common malignant tumor in men, and its mortality rate ranks second among various cancers; in Asia, its incidence rate is lower than that in Western countries, but it has shown a rapid upward trend in recent years. [0003] In addition to endocrine therapy (androgen resistance therapy) and bisphosphonate drug therapy, chemotherapy, external radiation therapy, external radionuclide internal radiation therapy and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08A61K31/7048A61P35/00
Inventor 周欣陈华国龚小见赵超先春黄志金
Owner GUIZHOU NORMAL UNIVERSITY
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