Biological enzyme demulsification method for extracting soybean oil and fat by aqueous enzymatic method

Abstract

The invention discloses a biological enzyme demulsification method for extracting soybean oil and fat by an aqueous enzymatic method and belongs to vegetable oil and fat extraction technologies. The method includes the following steps of (1) smashing puffed soybeans, mixing the smashed soybeans with water to obtain a mixed liquor, adding alkaline protease in the mixed liquor to perform an enzymolysis reaction, and after the enzymolysis, performing a centrifugal separation to obtain free oil, an emulsion, a hydrolysate and residues; (2) adding water and phospholipase in the emulsion obtained from the step (1) to stir to perform the enzymolysis reaction to obtain a reaction solution; and (3) adding alcohol in the reaction solution obtained from the step (2) to perform an ultrasonic processing, and then performing the centrifugal separation to obtain the soybean oil and fat. The biological enzyme demulsification method for extracting the soybean oil and fat by the aqueous enzymatic method has the advantages that the required processing equipment is simple, the demulsification time is short, the method is environment-friendly, and the demulsification rate is high.

Description

technical field [0001] The invention belongs to vegetable oil extraction and processing technology, and mainly relates to a biological enzyme demulsification method for extracting soybean oil by aqueous enzymatic method. Background technique [0002] In the process of extracting soybean oil by aqueous enzymatic method, the yield of free oil is low due to the emulsification of protein and oil, so we must find an appropriate demulsification method to recover oil from the emulsion, thereby increasing the yield of free oil, and the demulsification Good or bad directly affects the economic value of the entire process. [0003] Demulsification methods are divided into chemical demulsification, physical demulsification and biological demulsification. The chemical demulsification method is to add a chemical demulsifier; centrifugation, changing the temperature, applying an electrostatic field, and using a coalescing agent are all physical methods; the biological demulsification met...

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Problems solved by technology

In addition, foreign scholars mostly use organic solvents to directly extract the oil in the emulsion in order to investigate the effect of releasing the oil from the oil plant cells after enzymatic hydrolysis, but no further research on the technical level has been reported, and, now Some demulsification technologies have problems such as low demulsification rate, environmental pollution, high energy consumption, and cumbersome method steps.

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