Unlock instant, AI-driven research and patent intelligence for your innovation.

Endophytic fungus with preventive and treatment effects on soft rot of dendrobiums

A technology for endophytic fungi and soft rot, applied in the field of bioengineering, can solve the problems of restricting the development of dendrobium industry, labor and time-consuming, and lack of standard operating techniques

Active Publication Date: 2013-07-10
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When the disease is serious, soft rot can cause large-scale failure of cultivated crops, which seriously restricts the development of Dendrobium industry
At present, there are mainly two ways to prevent and treat the soft rot of Dendrobium plants: one is through cultivation management, such as controlling the humidity of the seedbed, ventilation, disinfection of water sources, and sunlight exposure treatment of the cultivation substrate, etc., which can play a role in the prevention and control of the disease. However, this kind of method is labor-intensive and time-consuming, the operation technology is not standardized, the controllability is poor, and the efficiency of disease control is not high; However, it is easy to cause drug resistance of pathogenic bacteria, and the extensive use of pesticides has a great effect on environmental pollution and ecological balance damage

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Biological Control of Dhs96 on Soft Rot of Dendrobium nobile

[0014] The endophytic fungus Dhs96 was cultured by PDA liquid fermentation and mixed with wheat bran solid substrate to make a solid inoculant. The soft rot pathogen of Dendrobium ultimum (Pythium ultimum) is activated and cultivated to collect spores and prepare 5×10 5 / ml of spore suspension, mixed with wheat bran solid matrix to make solid inoculant. Using bark as the cultivation substrate, when planting Dendrobium nobile seedlings, Dhs96 solid inoculant was inoculated to the roots of Dendrobium nobile seedlings. The potted seedlings are placed in a solar greenhouse, and the temperature is maintained at 20±2°C. Spray water after planting to facilitate the growth of endophytic fungi. Spray water every three days thereafter. The solid inoculant of pathogenic bacteria was sprayed around the Dendrobium nobile seedlings at 3 and 30 days after the inoculation of the fungus Dhs96. The negative control is the De...

Embodiment 2

[0016] Biological Control of Dhs96 on Soft Rot of Dendrobium officinale Seedlings

[0017] The endophytic fungus Dhs96 was cultured by PDA liquid fermentation and mixed with wheat bran solid substrate to make a solid inoculant. The soft rot pathogen of Dendrobium ultimum (Pythium ultimum) is activated and cultivated to collect spores and prepare 1×10 5 / ml of spore suspension, mixed with wheat bran solid matrix to make solid inoculant. Using bark as a cultivation substrate, when planting Dendrobium candidum seedlings, Dhs96 solid inoculant was inoculated to the roots of Dendrobium candidum seedlings. The potted seedlings are placed in a solar greenhouse, and the temperature is maintained at 20±2°C. Spray water after planting to facilitate the growth of endophytic fungi. Spray water every three days thereafter. The solid inoculant of pathogenic bacteria was sprayed around the Dendrobium officinale seedlings at 3 and 30 days after inoculation with the fungus Dhs96. The negative...

Embodiment 3

[0019] Biological Control of Dhs96 on Soft Rot of Dendrobium Drumstick Seedlings

[0020] The endophytic fungus Dhs96 was cultured by PDA liquid fermentation and mixed with wheat bran solid substrate to make a solid inoculant. The soft rot pathogen of Dendrobium ultimum (Pythium ultimum) is activated and cultivated to collect spores and prepare 5×10 4 / ml of spore suspension, mixed with wheat bran solid matrix to make solid inoculant. Using bark as the cultivation substrate, when planting D. Dendrobium seedlings, Dhs96 solid inoculant was inoculated to the roots of D. Dendrobium seedlings. The potted seedlings are placed in a solar greenhouse, and the temperature is maintained at 20±2°C. Spray water after planting to facilitate the growth of endophytic fungi. Spray water every three days thereafter. The solid inoculant of pathogenic bacteria was sprayed around the seedlings of Dendrobium candidum at 3 and 30 days after the inoculation of the fungus Dhs96. The negative control...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses an endophytic fungus with preventive and treatment effects on soft rot of dendrobiums. The fungus can be subjected to field planting at the root of dendrobiums, and is used for seedling raising or cultivation of dendrobiums. The endophytic fungus with preventive and treatment effects on soft rot of dendrobiums can effectively prevent the soft rod of dendrobiums from happening.

Description

Technical field [0001] The invention relates to the technical field of bioengineering, in particular to two endophytic fungal strains with biological control effects on soft rot of Dendrobium plants. Using these two fungi in the nursery or cultivation of Dendrobium plants can effectively prevent the occurrence of soft rot in Dendrobium plants. Background technique [0002] There are many traditional and valuable Chinese medicinal materials in orchid plants, such as Dendrobium officinale and Dendrobium nobile. According to the 2005 edition of the Chinese Pharmacopoeia, Dendrobium nobile LindL., D. candidum Wall (ex Lind1.) or D. fimbriatum Hook, var. oculatum Hook. and their similarities The fresh or dried stems of this species can be used as the traditional Chinese medicine Dendrobium, which has the effects of nourishing the stomach, nourishing fluid, nourishing yin and clearing away heat, improving the throat and eyesight, and regulating the immune function of the human body. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00A01G13/00C12R1/845
Inventor 郭顺星李向东陈晓梅王卉
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI