Taxane medicines albumin nanoparticle preparation for injection and preparation method thereof
A technology of albumin nanoparticles and taxanes, which is applied in the field of taxane drug albumin nanoparticle preparations for injection and its preparation. Sex and other issues, to achieve good clinical application prospects, improve anti-tumor effect, reduce the effect of dosage
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[0040] Example 1. Prescription process of paclitaxel albumin nanoparticles
[0041] Table 1 The proportion of each component in the formulation of paclitaxel-containing albumin nanoparticles (mg)
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[0044] Dissolve paclitaxel in prescriptions 1-30 in Table 1 in an appropriate amount of ethanol or other suitable solvents, dissolve the phospholipids in an appropriate amount of dichloromethane, and mix the two as the oil phase; dissolve albumin and antioxidants in an appropriate amount to remove Ionized water (water phase), drop the oil phase into the magnetically stirred water phase at room temperature or in an ice water bath, and continue to stir for a certain period of time, and then rotate and evaporate the ethanol and dichloromethane in a 40°C water bath to obtain paclitaxel-containing Albumin nanoparticles.
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[0045] Example 2. Prescription process of docetaxel albumin nanoparticles
[0046] Table 2 The proportion of each component in the formulation of docetaxel-containing albumin nanoparticles (mg)
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[0049] Dissolve docetaxel in prescriptions 1-30 in Table 2 in an appropriate amount of ethanol or other suitable solvents, dissolve the phospholipids in an appropriate amount of dichloromethane, and mix the two as the oil phase; dissolve albumin and antioxidants in an appropriate amount In deionized water (water phase), drop the oil phase into the magnetically stirred water phase at room temperature or in an ice water bath, and continue to stir for a certain period of time, and then rotate and evaporate in a water bath at 40°C to remove ethanol and dichloromethane. Albumin nanoparticles of paclitaxel.
Example Embodiment
[0050] Example 3 Pharmacodynamic test of paclitaxel-coated albumin nanoparticles on subcutaneous non-small cell lung cancer
[0051] Tumor cells: human non-small cell lung cancer A549 cells (purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences), cultured in DMEM medium containing 10% fetal bovine serum.
[0052] Animals: Balb / c nude mice, 4 weeks old, male. The test group and the negative control group had 6 nude mice in each group.
[0053] Tumor cell inoculation: use the right axillary subcutaneous inoculation model, take the vigorously growing A549 cells under aseptic conditions, digest the cells and resuspend them to about 2.0E7 / ml cell suspension, and inoculate 0.1 subcutaneously in the right axillary of each mouse ml cancer cell suspension.
[0054] The tumor volume and tumor inhibition rate were calculated with the same method as before.
[0055] Each group was administered according to the dosing schedule in Table 3 for two weeks.
[0056] Table 3 The dosing...
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