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Photosynthetic bacterium strain and use thereof

A technology of photosynthetic bacteria and strains, applied in the field of microorganisms, can solve the problems of low tolerance and inability to effectively promote the growth of vegetables, and achieve the effects of improving yield and quality, preventing soil acidification, and improving yield and quality

Active Publication Date: 2012-11-28
广东凯利生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the defects of low tolerance of existing photosynthetic bacteria and the inability to effectively promote the growth of vegetables, and provide a photosynthetic bacteria strain with acid resistance, alkali resistance, salt resistance, and antibiotic resistance, which can significantly improve the yield and quality of vegetables

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] colloidal rhododendron ( Rubrivivax gelatinosus ) Isolation, purification and identification of sbg11

[0015] Enrichment: Weigh 10 g of the soil sample into a triangular flask containing 50 mL of sterile water, fully oscillate and break up to make a suspension, and put it into the triangular flask at a ratio of 1:5 with the enriched nutrient solution, and cover A layer of sterile liquid paraffin (about 2 cm in height) forms an anaerobic environment, seals it, and places it in a light incubator at 30±2°C for 15-20 days. Yellow, green, red, purple, etc. grow on the bottle wall first. A variety of strains of different colors, and then covered by the dominant strain and stuck to the wall of the bottle. Take 100 μL of the bacterial suspension in the first-generation culture bottle and put it into 250 mL enriched nutrient solution for second-generation culture, and the culture method is the same as that of the first generation. After culturing for about 10 days, draw 10 μL...

Embodiment 2

[0032] Photosynthetic bacteria applied to lettuce

[0033] The jelly-shaped rhododendron ( Rubrivivax gelatinosus ) sbg11 single colony in 25mL of fresh LB liquid medium, 37°C, 180rpm shaking culture for 24h, then transfer the cultured liquid seed culture solution to the fermentation medium according to 3% inoculum size, 37°C, 180rpm shaking culture 28h, spare. The pot experiment was done with 4 treatments and 5 repetitions. I1: chemical fertilizer as base fertilizer, bacterial fertilizer as top dressing; I2: chemical fertilizer as base fertilizer, equal amount of blank nutrient solution without inoculation as top dressing; I3: only equal amount of fertilizer as base fertilizer, no top dressing; CK: water only without fertilization. The above four processes are consistent with other daily management. The results showed that the average value of lettuce dry weight was the highest in I1 treatment, which was 12.21% higher than I2, indicating that the application of bacteria ag...

Embodiment 3

[0036] Photosynthetic bacteria applied to pepper

[0037] The colloidal rhodoprolonga ( Rubrivivax gelatinosus ) sbg11 single colony in 25 mL of fresh LB liquid medium (same as Example 2), 37 ° C, 180 rpm shaking culture for 24 hours, and then transfer the cultured liquid seed culture solution to the fermentation medium according to the inoculum size of 3% , 37°C, shake culture at 180 rpm for 28h, set aside. In the field experiment, 4 treatments were performed, and 12 plots were replicated in 3 repetitions. Treatment 1: photosynthetic fertilizer 5mL·m -2 ;Treatment 2: photosynthetic fertilizer 10mL m -2 ; Treatment 3: sterilized photosynthetic fertilizer 10mL m -2 . The bacterial fertilizer is diluted 20 times with water and sprayed on the leaves of vegetables. The same amount of water was sprayed in the control area, and the other daily management of the above four communities was the same. The experimental results showed that treatment 1 flowered earlier than the cont...

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Abstract

The invention relates to a photosynthetic bacterium strain and a use thereof. The photosynthetic bacterium strain is named as (Rubrivivax gelatinosus) sbg11, is preserved in the China general microbiological culture collection (CGMCC) On May 7, 2012, and has a preservation number of CGMCC No.6085, wherein a 16SrDNA sequence is shown in the formula of SEQ ID NO: 1. The photosynthetic bacterium strain can obviously improve the output and the quality of vegetables and can be widely used for various vegetables. The output and the quality of vegetables such as lettuces, tomatoes and hot peppers treated by the photosynthetic bacterium strain are improved obviously.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a photosynthetic bacterial strain and application thereof. Background technique [0002] Photosynthetic bacteria are a class of microorganisms with functions such as carbon fixation, nitrogen fixation, dehydrogenation, and sulfide oxidation. In addition, photosynthetic bacteria themselves are living proteins, which are rich in amino acids, vitamins, growth-promoting factors, antiviral active factors, and coenzyme Q. And a variety of physiologically active substances, which have the functions of anti-lipid peroxidation, improving water quality, cultivating bait organisms, preventing and controlling diseases, improving survival rate, nitrogen fixation, and hydrogen production. The production of new energy and health care products are being applied more and more, becoming an indispensable part of modern biotechnology. [0003] However, the existing photosynthetic bacteria are generall...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F11/08A01G1/00C12R1/01
Inventor 黄秋生彭桂香杨芳刘光华谭志远田俊岭
Owner 广东凯利生物科技有限公司
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