Method for extracting natural algicide from Acorus calamus L and algae inhibition method

The technology of an algae inhibitor and water calamus, which is applied in the field of algae inhibitory active ingredients, can solve the problems of high cost, difficult separation and the like, and achieve the effects of low cost, strong environmental affinity, and simple and easy extraction method.

Inactive Publication Date: 2012-12-12
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the disadvantages of difficult separation and high cost in the actual use of existing plant algae inhibitors, the present invention provides a method for extracting algae inhibitory active ingredients from calamus calamus and an algae inhibitory method, which have a wide range of sources and good algae inhibitory effect ,low cost

Method used

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  • Method for extracting natural algicide from Acorus calamus L and algae inhibition method
  • Method for extracting natural algicide from Acorus calamus L and algae inhibition method

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Embodiment 1: The massive rhizomes of Calamus calamus are collected, dried and pulverized. Take by weighing 50 g of calamus massive rhizome after crushing, extract 2-3 times at 50 C, each 12 hours. After filtration, the petroleum ether extracts were combined and concentrated by rotary evaporation at 35°C to obtain algae-inhibiting active component A. The active component A was subjected to acute toxicity experiments on aseptically cultured single-cell Microcystis aeruginosa, population Microcystis aeruginosa, A. aeruginosa and Anabaena blooms. Several concentration gradients were set, and blank and three The groups were parallel, observed once every 24 hours, and observed continuously for three days. The results were expressed as 96-hour inhibition rates, and the results were as follows:

[0031] Algae-inhibiting active ingredient A, when the mass volume concentration reaches 50mg / L (therefore 1 liter of water contains 50mgA), its inhibition rate on single-cell Microcy...

Embodiment 2

[0032] Embodiment 2: The massive rhizomes of Calamus calamus are collected, dried and pulverized. Weigh 50 g of crushed calamus massive rhizomes, extract 2-3 times at 50 degrees, each time for 12 hours. After filtration, the ethyl acetate extracts were combined and concentrated by rotary evaporation at 35°C to obtain algae-inhibiting active component B.

[0033] The active component B was subjected to acute toxicity experiments on aseptically cultured single-cell Microcystis aeruginosa, population Microcystis aeruginosa, A. aeruginosa and Anabaena blooms. Several concentration gradients were set, and blank and three The groups were parallel, observed once every 24 hours, and observed continuously for three days. The results were expressed as 96-hour inhibition rates, and the results were as follows:

[0034] Algae-inhibiting active ingredient B, when the concentration reaches 50mg / L, its inhibition rate on single-cell Microcystis aeruginosa reaches 91%; when the concentration...

Embodiment 3

[0035] Embodiment 3: The massive rhizomes of Calamus calamus are collected, dried and pulverized. Weigh 50 g of crushed calamus massive rhizomes, extract 2-3 times at 50 degrees, each time for 12 hours. After filtration, the anhydrous ethanol extracts were combined and concentrated by rotary evaporation at 35°C to obtain algae-inhibiting active component C.

[0036] The active component C was subjected to acute toxicity experiments on aseptically cultured single-cell Microcystis aeruginosa, colony Microcystis aeruginosa, A. aeruginosa and Anabaena blooms. Several concentration gradients were set, and blank and three The groups were parallel, observed once every 24 hours, and observed continuously for three days. The results were expressed as 96-hour inhibition rates, and the results were as follows:

[0037] Algae-inhibiting active ingredient C, when the concentration reaches 50mg / L, its inhibition rate on single-cell Microcystis aeruginosa reaches 95%; when the concentration...

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Abstract

The invention discloses a method for extracting active components for algae inhibition from Acorus calamus L tubers. According to the invention, an organic solvent is directly used to extract the active components so as to obtain the algicide, or the organic solvent is used for sequential extraction to obtain the algicide. The direct extraction method of the organic solvent comprises the following concrete steps of: extracting washed, dried and crushed Acorus calamus L tubers for 2-5 times respectively by using petroleum ether, ethyl acetate and anhydrous ethanol, filtering, and condensing to obtain the active components A, B and C for algae inhibition. The sequential extraction method of the organic solvent comprises the following concrete steps of: extracting by the use of anhydrous ethanol to obtain an extract C, dissolving the extract C in 80% of a methanol aqueous solution, carrying out isopyknic extraction for 2-3 times by the use of petroleum ether, condensing the petroleum ether layer to obtain a first active component for algae inhibition, diluting residual methanol aqueous solution to 50% by the addition of water, carrying out isopyknic extraction for 2-3 times by the use of ethyl acetate, and condensing the ethyl acetate layer to obtain a second active component for algae inhibition. The extraction method is simple and easy to operate. The extracted active components, which are used as the algicide, are cheap and nontoxic, require low cost, and are suitable for practical popularization and application. The algicide can be used without the restrictions of region, solar term, temperature and the like.

Description

technical field [0001] The invention belongs to the field of water body pollution control, and particularly designs a method for algae-inhibiting active ingredients extracted from the massive stems of the natural plant Acorus calamus. Background technique [0002] There are two main ideas for water bloom prevention and control technology: control of nutrients such as nitrogen and phosphorus and control of algae. At present, algae control technology includes physical algae control technology, chemical algae control technology and biological algae control technology, and the most commonly used method is chemical method. The existing chemical algicides are mainly copper sulfate. The mechanism is to use the toxicity of copper ions to interfere with the photosynthetic reaction system of algae, so that it cannot carry out photosynthesis normally, and finally achieve the purpose of killing algae. However, copper ions are heavy metals. Ions, not selective, are absorbed or ingested ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/40A01P13/00A01P1/00
Inventor 张胜花赵静孙珮石
Owner YUNNAN UNIV
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