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Contrast agent and preparation method thereof

A technology of contrast agent and mixture, which is applied in the field of medical imaging and can solve the problems of reduced efficiency and sensitivity

Active Publication Date: 2012-12-12
SHENZHEN INST OF ADVANCED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When the diameter of nanoparticles is larger than 20 nm, they are easily phagocytized by the reticuloendothelial system (RES), resulting in a decrease in their efficiency and sensitivity

Method used

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  • Contrast agent and preparation method thereof
  • Contrast agent and preparation method thereof

Examples

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preparation example Construction

[0037] see figure 1 , a method for preparing a contrast agent, comprising the steps of:

[0038] Step S110: mix the chloroauric acid solution with a concentration of 1-100mmol / L and the bovine serum albumin solution with a concentration of 1-500mg / mL, then add a sodium hydroxide solution with a concentration of 0.1-10mol / L, and dissolve the chloroauric acid solution , the mixed solution of bovine serum albumin solution and sodium hydroxide solution was shaken and reacted for 3 to 100 hours at 0 to 100°C to obtain a solution containing gold nanoclusters, wherein the volume of chloroauric acid, bovine serum albumin and sodium hydroxide solution The ratio is 0.1~5:0.1~25:0.1~2.

[0039] Mixed chloroauric acid (HAuCl 4 ) solution and bovine serum albumin (BSA) solution, and then add sodium hydroxide solution to make chloroauric acid (HAuCl 4 ) solution, bovine serum albumin (BSA) solution and sodium hydroxide solution mixture is alkaline.

[0040] Using bovine serum albumin (B...

Embodiment 1

[0056] 1. Preparation of gold nanoclusters: Add 5mL of chloroauric acid solution (10mmol / L) to 5mL of BSA solution (50mg / mL, mix well, then add 0.5mL of 1mol / L NaOH solution, and place the mixture at 37°C Gold nanoclusters formed after incubation in a shaker (200 rpm) for 12 hours.

[0057] 2. Preparation of gold nanoclusters coupled with diethylenetriaminepentaacetic acid: Add 2mL of 2M PBS buffer (pH=9) to 40ml of a solution containing gold nanoclusters, then add 280mg of DTPACA powder, and use 5M NaOH solution Titrate, adjust the pH value to 6.0, and shake gently at room temperature for 2 hours to generate gold nanoclusters coupled with diethylenetriaminepentaacetic acid.

[0058] 3. Preparation of contrast agent: weigh 300mg gadolinium chloride (GdCl 3 .6H 2 O) Dissolve in 1mL of 1M HCl, and add to the above solution containing gold nanoclusters coupled with diethylenetriaminepentaacetic acid, titrate with 5M NaOH solution, adjust the pH value to 6.0, shake gently at roo...

Embodiment 2

[0067] Preparation of contrast agent

[0068] 1. Preparation of gold nanoclusters: Add 10 mL of chloroauric acid solution (5 mmol / L) into 10 mL of BSA solution (25 mg / mL), mix well, then add 1 mL of 0.5 mol / L NaOH solution, and place the mixture at 50 Gold nanoclusters were formed after incubation in a shaker at 300 rpm for 24 hours.

[0069] 2. Preparation of gold nanoclusters coupled with diethylenetriaminepentaacetic acid: add 2mL of 4M PBS (pH=9) to 50ml of gold nanocluster solution, then add 400mg of DTPACA powder, and titrate with 3M NaOH solution to adjust the pH value to 9.0, the reaction was gently shaken at room temperature for 1 hour to generate gold nanoclusters coupled with diethylenetriaminepentaacetic acid.

[0070] 3. Preparation of contrast agent: weigh 500mg gadolinium chloride (GdCl 3 .6H 2O) Dissolve in 0.5 mL of 1M HCl, and add to the above solution containing gold nanoclusters coupled with diethylenetriaminepentaacetic acid, titrate with 3M NaOH soluti...

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Abstract

The invention provides a contrast agent which comprises water and nanoparticles suspending in water and with a gold nano-cluster as a core and a diethylenetriamine pentaacetic acid-gadolinium chelating agent as a shell. The gold nano-cluster has the functions of near infrared fluorescence (NIR) and computed tomography (CT) imaging; gadolinium is a rare earth element responsive to nuclear magnetic resonance, and the diethylenetriamine pentaacetic acid-gadolinium chelating agent has a magnetic resonance imaging (MIR) function; the contrast agent provided by the invention fuses the gold nano-cluster and the gadolinium chelating agent together, is capable of NIR / CT / MRI three-mode radiography and can fuse near infrared fluorescence, computed tomography imaging and magnetic resonance imaging together. Furthermore, the nanoparticles have a diameter of mere 0.9 to 1.1 nm, so the contrast agent is hardly phagocytosed by a reticuloendothelial system (RES) of a living body and has high permeability and improved radiographic sensitivity. The invention also provides a preparation method for the contrast agent.

Description

technical field [0001] The invention relates to the field of medical imaging, in particular to a contrast agent and a preparation method thereof. Background technique [0002] Molecular imaging is the main research direction of tumor diagnosis today, and each imaging technology has its own independent imaging principle, structure and detection probe, and has different detection responsiveness to lesion tissue in vivo. It is difficult to obtain sufficient lesion site information with single-modality imaging techniques, and the detection results of each imaging technique are independent of each other, making it difficult to compare and analyze the data, making it difficult for researchers and clinicians to make test reports for patients. Therefore, integrating the advantages of various imaging technologies, developing multi-modal molecular imaging methods (acoustic, optical, thermal, magnetic, nuclear, etc.), and making up for the defects of single-modal imaging technology hav...

Claims

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Application Information

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IPC IPC(8): A61K49/06A61K49/18
Inventor 蔡林涛胡德红盛宗海张鹏飞高笃阳龚萍
Owner SHENZHEN INST OF ADVANCED TECH
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