Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for detecting phosphorus component in soil by using BD-liquid 31PNMR (pulse nuclear magnetic resonance)

A phosphorus component and soil technology, which is applied in the preparation of test samples and analysis by nuclear magnetic resonance, etc., can solve the problems of low soil phosphorus extraction rate, large chemical shift deviation, and low spectral resolution, and achieve an increase Effects of Accuracy, Increased Accuracy, Increased Resolution

Inactive Publication Date: 2012-12-12
SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
View PDF0 Cites 12 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the determination of phosphorus components in soil, there are still problems such as low soil phosphorus extraction rate, low spectral resolution, and large chemical shift deviations, resulting in poor spectral accuracy.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting phosphorus component in soil by using BD-liquid 31PNMR (pulse nuclear magnetic resonance)
  • Method for detecting phosphorus component in soil by using BD-liquid 31PNMR (pulse nuclear magnetic resonance)
  • Method for detecting phosphorus component in soil by using BD-liquid 31PNMR (pulse nuclear magnetic resonance)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Take 2.5g of air-dried soil samples passed through a 2mm sieve under different land use patterns (cornfield, paddy field, woodland and abandoned land), add 50ml NaOH-EDTA (preparation method: weigh 10g NaOH and dissolve in 600mL deionized water, Then add 18.612g Na 2 EDTA, the final volume was adjusted to 1000mL) after the mixture was shaken, it was centrifuged at 10000*g for 30 minutes. The supernatant after the centrifugation of the extract was taken 5-10ml each time, so that the total volume reached about 40ml, and then freeze-dried immediately. Before freeze-drying, add 5% sodium bicarbonate-dithionite buffer solution (preparation method: weigh 1.8482gNaHCO 3 with 3.8309gNa 2 S 2 o 4 Dilute to 200mL with deionized water and shake well, then quickly freeze it with a rotary freezer at a temperature below -40°C for about 20 minutes, so that the extract can be evenly frozen on the bottom of the freeze-drying bottle. Quickly transfer the frozen freeze-dried vial to ...

Embodiment 2

[0033] Take 2.5g of air-dried soil samples passed through a 2mm sieve from grassland soil (grazing, weeding) under different management methods, and add 50ml of NaOH-EDTA (preparation method: weigh 10g of NaOH and dissolve it in 600mL of deionized water, then add 18.612g of NaOH-EDTA 2 EDTA, the final volume was adjusted to 1000mL) after the mixture was shaken, it was centrifuged at 10000*g for 40 minutes. The supernatant after the centrifugation of the extract was taken 5-10ml each time, so that the total volume reached about 40ml, and then freeze-dried immediately. Before freeze-drying, add 4% sodium bicarbonate-dithionite buffer solution (preparation method: weigh 1.8482g NaHCO 3 with 3.8309g Na 2 S 2 o 4 Dilute to 200mL with deionized water) and shake well, then quickly freeze it with a rotary freezer at -45°C for about 30 minutes, so that the extract is evenly frozen on the bottom of the freeze-drying bottle. Quickly transfer the frozen freeze-dried vial to a freeze-d...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the leaching and the detection of a phosphorus component in the soil and particularly relates to a method for detecting a phosphorus component in soil by using BD-liquid 31PNMR (pulse nuclear magnetic resonance). The method comprises the following steps of: dissolving the soil to be detected by using NaOH-EDTA solution, centrifugally separating the dissolved solution to obtain soil phosphorus leach liquor, adding a sodium bicarbonate-hyposulphite buffer solution in the leach liquor, and carrying out freeze-drying treatment; and re-dissolving the powder obtained after the freezing treatment, and finally, detecting by using the 31PNMR to obtain the phosphorus component in the soil to be detected in quantitative and qualitative forms. The phosphorus in the soil is leached and detected by using a physical and chemical combined method, so that the resolution and the veracity of the 31PNMR are increased; and the method is suitable for identification and analysis of the phosphorus component in the soil.

Description

technical field [0001] The invention relates to the leaching and determination of soil phosphorus components, specifically a method using BD-liquid 31 P NMR method for the determination of soil phosphorus components. Background technique [0002] Phosphorus (P), as the central substance of plant energy transformation (ATP), cell structure (phospholipid), metabolism and signal transduction, is a nutrient element necessary for plant growth and development. Understand the characteristics of phosphorus components in soil and control them Utilization plays an important role in maintaining the balance of the ecosystem. Phosphorus in soil can be divided into two components: organic phosphorus and inorganic phosphorus. Because the determination of organic phosphorus compounds in soil is relatively difficult, the research on its determination method is later than that of inorganic phosphorus, and its development is also relatively slow. The soil organic phosphorus classification sy...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N24/08G01N1/28
Inventor 张艾明陈振华陈利军梁文举
Owner SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com