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Method for obtaining preparation solution for promoting rapid growth of chlorella from fallen leaves of Chinese white poplar

A technology of Populus tomentosa and Chlorella, applied in the direction of using microorganisms, adding compounds to stimulate growth, single-cell algae, etc., can solve the problems of limited utilization methods and low added value, and achieve simple process, low cost, Effects from a wide range of sources

Inactive Publication Date: 2012-12-26
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The fallen leaves of Populus tomentosa are generally collected for landfill or compost at present, with limited utilization and low added value

Method used

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  • Method for obtaining preparation solution for promoting rapid growth of chlorella from fallen leaves of Chinese white poplar

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Collect 1kg of leaves of Populus tomentosa, clean them up and grind them to 50 mesh with a pulverizer and store them in a sealed brown dry glass container. Weigh 50g of poplar tomentosa leaf powder and put it in a 500mL glass jar, soak it in 250mL distilled water under the stirring of a magnetic stirrer at room temperature for 48h. The obtained extract is filtered to remove particulate matter by vacuum filtration, and then filtered to remove impurities through a 0.22 μm filter membrane to obtain a preparation solution of the leaves of Populus tomentosa. Compared with the blank control, when the dosage is 5g / L, the growth promotion rate of Chlorella can reach 13.82 times on the 7th day; when the dosage is 10g / L, the growth promotion rate of Chlorella on the 7th day can be increased. The growth rate of Chlorella can reach 20.61 times; when the dosage is 20g / L, the growth promotion rate of Chlorella on the 7th day can reach 24.45 times.

Embodiment 2

[0023] Collect 1kg of leaves of Populus tomentosa, clean them up and grind them to 50 mesh with a pulverizer and store them in a sealed brown dry glass container. Weigh 50g of poplar tomentosa leaf powder and put it in a 500mL glass jar, soak it in 250mL of 40°C distilled water for 0.5h, and then cook it in an electric furnace for 4h. The obtained extract is filtered to remove particulate matter by vacuum filtration, and then filtered to remove impurities through a 0.22 μm filter membrane to obtain a preparation solution of the leaves of Populus tomentosa. Compared with the blank control, when the dosage is 5g / L, the growth promotion rate of Chlorella on the 7th day can reach 10.16 times; when the dosage is 10g / L, the growth promotion rate of Chlorella on the 7th day can reach 10.16 times. The growth rate of Chlorella can reach 21.14 times; when the dosage is 20g / L, the growth promotion rate of Chlorella on the 7th day can reach 27.66 times.

Embodiment 3

[0025] Collect 1kg of leaves of Populus tomentosa, clean them up and grind them to 50 mesh with a pulverizer and store them in a sealed brown dry glass container. Weigh 12.5g of Poplar tomentosa fallen leaves powder into a 500mL glass jar, and add 250mL of distilled water. Then add sulfuric acid to adjust the pH value to 2-3, place it at a high temperature of 100 °C for 1 h, and then neutralize it with ammonia water to a pH value of 7.0. The obtained extract is filtered to remove particulate matter by vacuum filtration, and then filtered to remove impurities through a 0.22 μm filter membrane to obtain a preparation solution of the leaves of Populus tomentosa. Compared with the blank control, when the dosage is 5g / L, the growth promotion rate of Chlorella can reach 1.49 times on the 7th day; when the dosage is 10g / L, the growth promotion rate of Chlorella on the 7th day can reach 1.49 times. The growth rate of Chlorella can reach 3.54 times; when the dosage is 20g / L, the growt...

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Abstract

The invention discloses a method for obtaining a preparation solution for promoting rapid growth of chlorella from fallen leaves of Chinese white poplar, belonging to the fields of biomass resources and biological energy sources. In order to solve the problem of rapid growth of a novel biomass energy source algae chlorella, and search a substance capable of promoting growth, the invention discloses a method for obtaining the preparation solution for promoting rapid growth of the chlorella from the fallen leaves of the Chinese white poplar. The method comprises the following steps: collecting the fallen leaves of the Chinese white poplar, cleaning and crushing the fallen leaves into 40-100 meshes; weighing fallen leaves powder in a glass jar; digesting by distilled water at normal temperature, boiling to extract by the distilled water, and processing the pH value to 2-3 by sulfuric acid acidification (1h, 12h for 2d), or processing the pH value to 4-5 by sulfuric acid acidification (1h, 12h for 2d) to obtain an extract, and filtering and removing grains of the extract to obtain the preparation solution of the fallen leaves of the Chinese white poplar. Each preparation solution can obviously promote chlorella growth, and the preparation solution obtained from the distilled water in a boiling manner has the best algal promoting effect. The algal promoting effect is obvious; the pollution of fallen leaves waste on the environment is removed; a high-value material is fully utilized; and the possibility for industrially and rapidly cultivating the chlorella is provided.

Description

technical field [0001] The invention belongs to the field of biomass resource utilization and bioenergy, in particular to a method for obtaining a preparation solution for promoting the rapid growth of Chlorella from leaves of Populus tomentosa. Background technique [0002] With the increasing shortage of petrochemical resources, energy issues have begun to attract attention, and the development of biomass resources to obtain bioenergy has also become a research hotspot. The use of plant or animal sources to produce bioenergy may lead to problems in the world's food supply, and finding a more ideal source of bioenergy has become a bottleneck for technological development. Chlorella is an important type of microalgae with high oil production and high protein. It has high oil production rate and is rich in protein, lipid, polysaccharide, edible fiber and trace elements. It can directly treat industrial waste gas to achieve emission reduction effect and has competitive growth ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/38C12N1/22C12N1/12
Inventor 洪喻上官小亚秦墨梅
Owner BEIJING FORESTRY UNIVERSITY
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