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Formula of Pavlova viridid medium and three-stage culture method

A green Pavlova and culture medium technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of complex equipment and high cost, and achieve the effect of easy control, low cost and fast growth

Inactive Publication Date: 2015-01-07
LINYI UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Some scholars suggest filling with air and CO 2 Mixed gas, the ratio is V air: VCO 2 =15:1 (volume ratio), but the equipment is complicated and the cost is too high, so it is better to directly use the air pump to inflate the air

Method used

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  • Formula of Pavlova viridid medium and three-stage culture method
  • Formula of Pavlova viridid medium and three-stage culture method
  • Formula of Pavlova viridid medium and three-stage culture method

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Embodiment Construction

[0020] Culture medium preparation method

[0021] 1) Ferric citrate is difficult to dissolve, you can add a small amount of tap water and heat it to 80-90°C on the stove, and keep stirring until it completely melts;

[0022] 2) 920 plant growth stimulant (ie gibberellin) is a white crystalline powder, which is difficult to dissolve in water. It can be fully dissolved with alcohol first (generally, 50 ml of alcohol or 60-degree liquor per gram is dissolved for more than 1 hour), and then Dilute with water according to the required concentration;

[0023] 3) During the process of adding nutrient salts, the water needs to be continuously stirred, and various nutrient salts are added in the order provided in the formula to avoid chemical reactions;

[0024] 4) All kinds of nutrient salts, especially f / 2 trace element solution and f / 2 vitamin solution, need to be made into mother liquor in advance;

[0025] 5) After adding all the nutrient salts, inflate and stir for 15 minutes b...

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Abstract

The invention discloses a formula of Pavlova viridid medium and a three-stage culture method, and relates to the field of marine microalgae culture. MAV culture medium formula traditionally used to culture Pavlova viridid has slow growth rate and low yield. According to my experiments, 108mg / L of urea substitutes nitrate, and is added with 500mg / L of NaHCO3 and supplemented with 5 IU / L of 920 growth hormone (gibberellin), f / 2 of trace elements and f / 2 of vitamins. The medium has more balanced and comprehensive nutrition, greatly accelerates the growth rate of Pavlova viridid, and increases the yield by 86%. As for culture of the Pavlova viridid, a glass crock is substituted by a white plastic bucket in a secondary culture, and an open cement pool is substituted by a plastic film suspension sling in a tertiary culture. The method has advantages of easy operation, little pollution, easy control, flexibility, no cutting, better security and reliability, good transparency, low cost, fast growth and high yield.

Description

technical field [0001] The invention belongs to the field of marine microalgae cultivation, and in particular relates to a green Pavlova culture medium composition and a tertiary cultivation method. Background technique [0002] Pavlova viridid ​​is a kind of marine microalgae, belonging to Chrysophyta, Purinella, Pavlophyceae, Pavlovaceae, and Pavlova genus. It has no cell wall, small individual, fast reproduction and rich nutrition. It is suitable for Ingestion and digestion of aquatic animal larvae, especially rich in docosahexaenoic acid EPA, which is necessary for the growth and development of aquatic economic animal larvae, is a high-quality single-celled algae bait, widely used in shrimp, crab, shellfish larvae and seawater Artificial rearing of larvae. Because EPA and DHA are also essential fatty acids for the human body, they can prevent and treat many diseases, and their value has been paid more and more attention by scientific researchers. [0003] The cultivati...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12R1/89
Inventor 王培磊
Owner LINYI UNIVERSITY