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Continuous gradient composite scaffold and preparation method thereof

A composite scaffold and gradient technology, applied in the field of biomedical materials and their preparation, can solve problems such as interface defects, and achieve the effects of good mechanical properties, improved biocompatibility, and good biocompatibility

Active Publication Date: 2014-07-02
NINGBO INST OF MATERIALS TECH & ENG CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The invention provides a continuous gradient composite scaffold, which solves the problem of interface defects of traditional multilayer scaffolds, enables the scaffold to have high strength and better biocompatibility, and better meets the multi-level requirements of the natural structure of osteochondral

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example 1

[0033] γ-Fe 2 o 3 Preparation of / nHAP nanoparticles: using N 2 The method of bubble blowing removes the oxygen dissolved in 30ml deionized water, and then 1.85mmol FeCl 2 4H 2 O and 3.7 mmol FeCl 3 ·6H 2 O was added to the above oxygen-free deionized water until it was completely dissolved to form an aqueous iron salt solution, and the iron salt solution was added to 10ml of NH 4 In OH, 90°C water bath and continuous magnetic stirring 300r / min, after 1h, the generated part is γ-Fe 2 o 3 stock solution of nanoparticles. In part for γ-Fe 2 o 3 In the original solution of nanoparticles, 33.7mmol Ca(NO 3 ) 2 4H 2 O (adjust pH=11) and 20mmol (NH 4 ) 2 HPO 4 (adjust pH = 11), 90 ° C water bath and continuous magnetic stirring 300r / min, after 2 hours, then aging at room temperature for 72 hours to obtain a magnetic composite nanoparticle solution of nano-hydroxyapatite / ferric oxide.

[0034] Place the magnet at the bottom of the beaker containing the magnetic composi...

preparation example 2

[0036] Fe 3 o 4 Preparation of / nHAP nanoparticles: using N 2 The method of bubble blowing removes the oxygen dissolved in 30ml deionized water, and then 1.85mmol FeCl 2 4H 2 O and 3.7 mmol FeCl 3 ·6H 2 O was added to the above oxygen-free deionized water until it was completely dissolved to form an aqueous iron salt solution, and the iron salt solution was added to 10ml of NH 4 In OH, stir magnetically at 300r / min at room temperature 25°C, and Fe will be generated after 1h 3 o 4 Nanoparticle stock solution. in Fe 3 o 4 In the original solution of nanoparticles, 33.7mmol Ca(NO 3 ) 2 4H 2 O (adjust pH=11) and 20mmol (NH 4 ) 2 HPO 4 (adjust pH=11), stir magnetically at 25°C for 300r / min, after 2h, and then age at room temperature for 24h to obtain nano-hydroxyapatite / ferric oxide magnetic composite nanoparticles (Fe 3 o 4 / nHAP) solution.

[0037] Place the magnet at the bottom of the beaker containing the nano-hydroxyapatite / ferric oxide magnetic composite nan...

Embodiment 1

[0039] 1) Dissolve 5.0g PVA (polymerization degree 1750±50, Sinopharm Chemical Reagent Shanghai Co., Ltd.) in 40.0g deionized water, heat to dissolve to obtain A1 solution, and then dissolve 0.5g CH in 25.0g deionized water Obtain A2 solution, A1 solution and A2 solution are mixed, obtain the polymer solution of 70.5g;

[0040] 2) Disperse 5.5 g of pure m-nHAP prepared in Preparation Example 1 in 134.7 g of deionized water, and obtain 140.2 g of magnetic composite nanoparticle suspension by ultrasonic dispersion;

[0041] 3) Mix the polymer solution in step 1) and the magnetic composite nanoparticle suspension in step 2) uniformly to obtain a mixed solution, inject the mixed solution into a mold, and induce it by a magnetic field with a magnetic field strength of 8000 Gauss before gelation The m-nHAP in the mixture was distributed in a continuous gradient for 120 hours, and then the mixture was gelled by cyclic freezing and thawing. In the method of cyclic freezing and thawing...

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PUM

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Abstract

The invention discloses a continuous gradient composite scaffold, and relates to the field of biomedical materials. The composite scaffold consists of hydrogel and magnetic composite nano particles; the weight percentages of the magnetic composite nano particles at the top and the bottom of the continuous gradient composite scaffold are respectively 0 percent and 10 to 70 percent; the weight percentage of the magnetic composite nano particles is gradually increased from the top of the continuous gradient composite scaffold to the bottom to form continuous gradient distribution; the hydrogel is hydrogel of polyvinyl alcohol and natural polysaccharide; and the magnetic composite nano particles are magnetic composite nano particles of an oxide of nano hydroxyapatite / iron. The continuous gradient composite scaffold improves cell compatibility by adding natural polysaccharide, has high strength and high biocompatibility, and can well meet the multilevel requirement of an osteochondral natural structure. The invention also discloses a preparation method for the continuous gradient composite scaffold. The preparation method is easy to implement and control, and has a broad application prospect.

Description

technical field [0001] The invention belongs to the field of biomedical materials and their preparation, and in particular relates to a continuous gradient composite support for osteochondral defect repair and a preparation method thereof. Background technique [0002] Natural osteochondral is a multi-layered structure of cartilage layer, calcified layer and subchondral bone layer. The repair of osteochondral defects by osteochondral tissue engineering has gone through three stages: single-layer scaffold, double-layer scaffold and three-layer scaffold. The design of a single-layer scaffold only considers the upper cartilage layer and ignores the lower bone layer, which is difficult to meet the structural requirements of natural osteochondral. Therefore, it is inevitable to construct a double-layer scaffold to repair osteochondral defects. The double-layer stent considers the bionic simulation of the upper cartilage structure and the lower bone structure, and has achieved cer...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/46A61L27/26
Inventor 侯瑞霞付俊程亚军张国花
Owner NINGBO INST OF MATERIALS TECH & ENG CHINESE ACADEMY OF SCI
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