Mupirocin purification method

A mupirocin and purification method technology, which is applied in the field of mupirocin purification, can solve the problems of large amount of organic solvent usage, many extraction steps, and low purity, and achieve energy saving, production cost reduction, and excellent product quality Effect

Inactive Publication Date: 2013-01-09
BEIJING RENFENG TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The yield of the patented product is low, and the product purity is not high at the same time
[0007] The above method has many extraction steps, large amount of organic solvent used, low product yield, low purity, etc., which is difficult to meet the needs of industrialization of mupirocin

Method used

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  • Mupirocin purification method

Examples

Experimental program
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Effect test

Embodiment 1

[0036] Measure 100L of fermentation broth, add 1M hydrochloric acid, adjust the pH value to 4.0, stir for 20 minutes, and centrifuge at 3000r / min to obtain mycelium, which is set aside. Add 2 times the volume of methanol to the obtained mycelium after the acidification treatment, stir evenly, soak for 4 hours, and keep the filtrate for future use after suction filtration.

[0037] Select the nanofiltration membrane with a cut-off molecular weight of 500-600 for the first nanofiltration concentration, set the inlet and outlet temperatures at room temperature, and filter the organic solvent to obtain the filtrate with a feed rate of 1.0L / h and an operating pressure of 0.5MPa. Concentrate by nanofiltration, set aside.

[0038] Add 5% polystyrene divinylbenzenesulfonate DOW[XFS.43278.002] sodium strong acid resin to the nanofiltration concentrate, stir at room temperature for 6 hours, rinse the resin with deionized water, and then use a sodium hydroxide solution with a pH of 10.0 ...

Embodiment 2

[0043] Measure 100L of fermentation broth, add 1M hydrochloric acid, adjust the pH value to 4.5, stir for 20min, and centrifuge at 3000r / min to obtain mycelium, which is set aside. Add 2 times the volume of methanol to the obtained mycelium after the acidification treatment, stir evenly, soak for 4 hours, and keep the filtrate for future use after suction filtration.

[0044] Select the nanofiltration membrane with a cut-off molecular weight of 500-600 for the first nanofiltration concentration, set the inlet and outlet temperatures at room temperature, and filter the organic solvent to obtain the filtrate with a feed rate of 1.2L / h and an operating pressure of 0.6MPa. Concentrate by nanofiltration, set aside.

[0045] Add 5% polystyrene divinylbenzenesulfonate DOW[XFS.43278.002] sodium strong acid resin to the nanofiltration concentrate, stir at room temperature for 8 hours, rinse the resin with deionized water, and then use a sodium hydroxide solution with a pH of 10.5 Deso...

Embodiment 3

[0050] Measure 100L of fermentation broth, add 1M acetic acid, adjust the pH value to 4.2, stir for 20min, and centrifuge at 3000r / min to obtain mycelium, which is set aside. Add 2 times the volume of methanol to the obtained mycelium after the acidification treatment, stir evenly, soak for 4 hours, and keep the filtrate for future use after suction filtration.

[0051] Select the nanofiltration membrane with a cut-off molecular weight of 500-600 for the first nanofiltration concentration, set the inlet and outlet temperatures at room temperature, and filter the organic solvent to obtain the filtrate with a feed rate of 1.0L / h and an operating pressure of 0.4MPa. Concentrate by nanofiltration, set aside.

[0052] Add 5% polystyrene divinylbenzenesulfonate DOW[XFS.43278.002] sodium strong acid resin to the nanofiltration concentrate, stir at room temperature for 7 hours, rinse the resin with deionized water, and then use a sodium hydroxide solution with a pH of 10.0 Desorb, co...

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Abstract

The invention discloses a mupirocin purification method and belongs to biotechnology. A high-purity mupirocin product is obtained by using structural characteristics of mupirocin, extracting by composite solvents and regulating the pH (potential of hydrogen) value to remove micromolecules in fermentation liquor and by means of nanofiltration, ion exchange, column chromatography and recrystallization, and the product purity is above 98% by testing so that the product can satisfy the demands of the pharmaceutical industry. The mupirocin purification method has the advantages of advanced technology, simple process, high yield, low cost, high mupirocin purity and the like, and is beneficial to improvement of production technology and industrialization level of the mupirocin in China.

Description

1. Technical field [0001] The invention belongs to the field of biotechnology, in particular to a method for purifying mupirocin. 2. Background technology [0002] Mupirocin, also known as pseudomonosporic acid A (pseudomonic acid A), bactroban, BRL4910A, is a naturally occurring broad-spectrum antibiotic, produced by immersion and fermentation of Pseudomonas fluorescens, and has a special structure. No other antibiotics were associated. Such substances produced by Pseudomonas fluorescens include pseudomycin acids A, B, C, and D. Pseudomonas A is its main active part, accounting for 90-95%. Mupirocin is a competitive inhibitor of isoleucyl t-RNA synthetase in bacteria, which inhibits protein synthesis. The isoleucine transfer ribonucleic acid cannot be formed, so the isoleucine cannot be transported to the ribosome and transferred to the growing protein chain, resulting in the stop of protein synthesis. It can achieve and maintain a high drug concentration on the surface...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D407/06
Inventor 王鹏丛倩千
Owner BEIJING RENFENG TECH
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