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Preparation of food-grade natural isorhodanate by application of supercritical CO2 extraction technology

A technology of isothiocyanate, extraction technology, applied in application, food preparation, food science, etc., can solve problems such as solvent residues

Inactive Publication Date: 2013-01-16
ZHEJIANG UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with the traditional solvent extraction method, the yield of isothiocyanate in the present invention is increased by 51.8%, and the solvent residue problem caused by solvent extraction is solved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0007] Embodiment 1: Preparation of food-grade natural isothiocyanate

[0008] Weigh 500g of broccoli seeds, grind them into powder, add petroleum ether to soak, overnight, and degrease. Recovery of petroleum ether. The seed powder was added into 0.01 mol / L phosphate buffer solution with 3 times volume and pH value of 7.2 for enzymatic hydrolysis, and kept in a water bath at 42°C for 63 minutes. Then the enzymatic solution was dried in a vacuum freeze-drying oven. Put the dry seed powder into a 1L extraction cylinder, set the extraction temperature to 45°C, the extraction time to 90min, the extraction pressure to 20Mpa, CO 2 The flow rate is 0.5L / min, the temperature of separator I is 30°C, and the temperature of separator II is 25°C. After the extraction is completed, the extract is taken and packaged for storage.

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Abstract

A technology for preparing isorhodanate from Broccoli seed by the application of a supercritical CO2 extraction technology belongs to the technical field of biochemistry. The main preparation process comprises the following steps of: milling Broccoli seed, degreasing, carrying out enzymatic hydrolysis, carrying out freeze drying, carrying out supercritical CO2 extraction, and separating to obtain an isorhodanate extract which is rich in benzyl isorhodanate and sulforaphane. The product is safe and nontoxic, has no side-effect, and can be used as a product ingredient of food, health food and medicines. As the supercritical CO2 extraction technology provided by the invention replaces a traditional solvent extraction technology, the yield of isorhodanate in the obtained extract is 0.513% and is higher than that of a solvent extraction method by 51.8%. In addition, the product safety problem caused by extraction solvent residual is solved. The isorhodanate provided by the invention can be applied in production ingredients of various common food, health food and medicinal products, and has good effects of raising antioxidation and antisenescence of food and preventing and treating tumor.

Description

technical field [0001] Application of supercritical CO 2 The preparation of food-grade natural isothiocyanate by extraction technology belongs to the technical field of biochemistry. Background technique [0002] There are two main sources of isothiocyanate: artificial synthesis and natural product extraction. Foreign isothiocyanate artificially synthesized high-purity products are expensive. Natural isothiocyanates widely exist in cruciferous vegetables, such as kale, Brussels sprouts, broccoli, garden celery, kale, and cabbage, etc., and are the degradation of glucosinolates under the action of plant endogenous myrosinase product. Epidemiological studies have found that isothiocyanates are effective cancer inhibitors, such as liver cancer, esophageal cancer, lung cancer and intestinal cancer. At present, there are few natural isothiocyanate products in China, and the yield is low. In addition, the residual problem of extraction solvent has not been well resolved, whic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/212A23L1/29C07C331/16A23L19/00A23L33/00
CPCY02P20/54
Inventor 袁海娜
Owner ZHEJIANG UNIVERSITY OF SCIENCE AND TECHNOLOGY
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